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Cytometry and Antibody Technology (Flow Cytometry)

Facility Details

About This Facility

Services and Equipment

Publications

Awards & Associations

Metadata

University of Chicago

924 E 57th St

Room R022

Chicago, IL 60637

United States of America

https://voices.uchicago.edu/ucflow/

cite this facility

Quicklinks:

https://coremarketplace.org/RRID:SCR_017760

Primary Contact:

David Leclerc

Last Updated: 10/11/2022

Facility RRID

RRID:SCR_017760

Facility Details

CAT provides cutting-edge cytometry and antibody technology supported by expert personnel, high-quality monoclonal antibodies and technical consultation and training of researchers.

Facility Policies

Services are offerred outside of University of Chicago

Consulting is offerred outside of University of Chicago

(To see more facility information, click the in the upper right)

Services offered:

Assays and Measurements

Flow Cytometric Analysis

Light Scattering

Monoclonal Antibody

Outreach

Protocol Development/Clinical Trial Coordination

Shared Instrumentation Oversight & Maintenance

Tissue Culture

Facility Equipment

Agilent Penteon

Flow Cytometer

Agilent Quanteon

Flow Cytometer

Amnis (Luminex) Image Stream MRKII

Imaging flow cytometer

BD BioSciences FACS Aria III

Cell sorter

BD BioSciences FACSAria Fusion

Cell sorter

BD BioSciences LSR Fortessa (2)

Flow cytometer

BD BioSciences LSRII

Flow cytometer

Cytek Aurora (2)

Spectral Flow Cytometer

Luminex Luminex 200

Multiplexing platform

Miltenyi Tyto

Cell Sorter

MSD Quickplex SQ120

Multiplexing platform

Standard BioTools Helios

Mass Cytometry

ThermoFisher Attune NxT

Flow cytometer

ThermoFisher Bigfoot

Cell sorter

No additional equipment has been listed

(To see more facility information, click the in the upper right)

Publications

Publications associated with this facility (Click To View):

1.) Baeten JT, Liu W, Preddy IC, Zhou N, McNerney ME (2022 May). CRISPR screening in human hematopoietic stem and progenitor cells reveals an enrichment for tumor suppressor genes within chromosome 7 commonly deleted regions. Leukemia, 36(5), 1421-1425. doi: 10.1038/s41375-021-01491-z. PMID: 35034954.

2.) Krishnan M, Senagolage MD, Baeten JT, Wolfgeher DJ, Khan S, Kron SJ, McNerney ME (2022 Jan 7). Genomic studies controvert the existence of the CUX1 p75 isoform. Scientific reports, 12(1), 151. doi: 10.1038/s41598-021-03930-4. PMID: 34997000.

3.) Carr T, McGregor S, Dias S, Verykokakis M, Le Beau MM, Xue HH, Sigvardsson M, Bartom ET, Kee BL (2022). Oncogenic and Tumor Suppressor Functions for Lymphoid Enhancer Factor 1 in E2a^-/- T Acute Lymphoblastic Leukemia. Frontiers in immunology, 13(), 845488. doi: 10.3389/fimmu.2022.845488. PMID: 35371057.

4.) Changrob S, Fu Y, Guthmiller JJ, Halfmann PJ, Li L, Stamper CT, Dugan HL, Accola M, Rehrauer W, Zheng NY, Huang M, Wang J, Erickson SA, Utset HA, Graves HM, Amanat F, Sather DN, Krammer F, Kawaoka Y, Wilson PC (2021 Dec 21). Cross-Neutralization of Emerging SARS-CoV-2 Variants of Concern by Antibodies Targeting Distinct Epitopes on Spike. mBio, 12(6), e0297521. doi: 10.1128/mBio.02975-21. PMID: 34781736.

5.) Chao JL, Korzinkin M, Zhavoronkov A, Ozerov IV, Walker MT, Higgins K, Lingen MW, Izumchenko E, Savage PA (2021 Sep 21). Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma. Cell reports. Medicine, 2(9), 100399. doi: 10.1016/j.xcrm.2021.100399. PMID: 34622236.

6.) Wilson P, Changrob S, Fu Y, Guthmiller J, Halfmann P, Li L, Stamper C, Dugan H, Accola M, Rehrauer W, Zheng NY, Huang M, Wang J, Erickson S, Utset H, Graves H, Amanat F, Sather DN, Krammer F, Kawaoka Y (2021 Jul 19). Cross neutralization of emerging SARS-CoV-2 variants of concern by antibodies targeting distinct epitopes on spike. Research square, (), . pii: rs.3.rs-678247. doi: 10.21203/rs.3.rs-678247/v1. PMID: 34312615.

7.) Rao E, Hou Y, Huang X, Wang L, Wang J, Zheng W, Yang H, Yu X, Yang K, Bugno J, Ding X, Vokes E, Fu YX, Weichselbaum RR, Liang HL (2021 Jun 15). All-trans retinoic acid overcomes solid tumor radioresistance by inducing inflammatory macrophages. Science immunology, 6(60), . pii: eaba8426. doi: 10.1126/sciimmunol.aba8426. PMID: 34723044.

8.) Dugan HL, Stamper CT, Li L, Changrob S, Asby NW, Halfmann PJ, Zheng NY, Huang M, Shaw DG, Cobb MS, Erickson SA, Guthmiller JJ, Stovicek O, Wang J, Winkler ES, Madariaga ML, Shanmugarajah K, Jansen MO, Amanat F, Stewart I, Utset HA, Huang J, Nelson CA, Dai YN, Hall PD, Jedrzejczak RP, Joachimiak A, Krammer F, Diamond MS, Fremont DH, Kawaoka Y, Wilson PC (2021 Jun 8). Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets. Immunity, 54(6), 1290-1303.e7. . PMID: 34022127.

9.) Maisel K, Hrusch CL, Medellin JEG, Potin L, Chapel DB, Nurmi H, Camacho DF, Gleyzer R, Alitalo K, Sperling AI, Swartz MA (2020 Jun 9). Pro-lymphangiogenic VEGFR-3 signaling modulates memory T cell responses in allergic airway inflammation. Mucosal immunology, 6(32), eaba0995. doi: 10.1038/s41385-020-0308-4. ID: 32518367.

10.) Williams JB, Li S, Higgs EF, Cabanov A, Wang X, Huang H, Gajewski TF (2020 Jan 30). Tumor heterogeneity and clonal cooperation influence the immune selection of IFN-γ-signaling mutant cancer cells. Nature communications, 11(1), 602. doi: 10.1038/s41467-020-14290-4. ID: 32001684.

11.) Hrusch CL, Stein MM, Gozdz J, Holbreich M, von Mutius E, Vercelli D, Ober C, Sperling AI (2019 Nov). T-cell phenotypes are associated with serum IgE levels in Amish and Hutterite children. The Journal of allergy and clinical immunology, 144(5), 1391-1401.e10. doi: 10.1164/rccm.201907-1343LE. ID: 31401285.

12.) Krishack PA, Louviere TJ, Decker TS, Kuzel TG, Greenberg JA, Camacho DF, Hrusch CL, Sperling AI, Verhoef PA (2019 Mar 21). Protection against Staphylococcus aureus bacteremia-induced mortality depends on ILC2s and eosinophils. JCI insight, 4(6), 304-306.e4. doi: 10.1165/rcmb.2019-0191ST. ID: 30721149.

13.) Schoettler N, Hrusch CL, Blaine KM, Sperling AI, Ober C (2019). Transcriptional programming and T cell receptor repertoires distinguish human lung and lymph node memory T cells. Communications biology, 2(4), 411. doi: 10.1038/s42003-019-0657-2. ID: 31754641.

14.) Bonham CA, Hrusch CL, Blaine KM, Manns ST, Vij R, Oldham JM, Churpek MM, Strek ME, Noth I, Sperling AI (2019). T cell Co-Stimulatory molecules ICOS and CD28 stratify idiopathic pulmonary fibrosis survival. Respiratory medicine: X, 1(8), 1041-1042. pii: 100002. doi: 10.1016/j.yrmex.2019.100002. ID: 32455343.

15.) Greenberg JA, Hrusch CL, Jaffery MR, David MZ, Daum RS, Hall JB, Kress JP, Sperling AI, Verhoef PA (2018 Apr 25). Distinct T-helper cell responses to Staphylococcus aureus bacteremia reflect immunologic comorbidities and correlate with mortality. Critical care (London, England), 22(1), 107. doi: 10.1186/s13054-018-2025-x. ID: 29695270.

16.) Williams JW, Ferreira CM, Blaine KM, Rayon C, Velázquez F, Tong J, Peter ME, Sperling AI (2018). Non-apoptotic Fas (CD95) Signaling on T Cells Regulates the Resolution of Th2-Mediated Inflammation. Frontiers in immunology, 9(12), 2521. doi: 10.3389/fimmu.2018.02521. ID: 30443253.

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Associations

Cancer Center

ABRF

Association of Biomolecular Resource Facilities

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Metadata

Links

All the links listed resolve to this core listing

https://coremarketplace.org/?FacilityID=293
https://coremarketplace.org/RRID:SCR_017760

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

Cytometry, antibody, technology, project, design, data, acquisition, analysis, service, core

Resource Type:

core facility, service resource, access service resource

Citation:

Chicago University Cytometry and Antibody Technology Core Facility (RRID:SCR_017760)

Cytometry and Antibody Technology (Flow Cytometry)

Quicklinks:

Primary Contact:

Facility RRID

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

MWACD

ISAC

CTSA

Cancer Center

ABRF

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

(To see more facility information, click the in the upper right)

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