The CoreMarketplace: BIDMC-Immunostaining and Microscopy Core

The BIDMC Immunostaining and Microscopy Core offers consultation and service for tissue immunostaining with paraffin or frozen sections, and for training to use confocal and widefield microscopes. All service work and training is fee-for-service.

Facility Policies

Services are offerred outside of Beth Israel Deaconess Medical Center

Consulting is offerred outside of Beth Israel Deaconess Medical Center

(To see more facility information, click the in the upper right)

Services offered:

Biological Photography/Photomicrography

Confocal Microscopy

Fluorescence TUNEL Staining-Paraffin Sections

Fluorescence-multi-label Using Paraffin Sections

Fluorescence-single Label Using Paraffin Sections

Immunoperoxidase (IPOX) Using Frozen Sections

IPOX-Paraffin Sections

Labeling Of Frozen Sections Using A Single Fluorescence Label

Labeling Of Frozen Sections Using Multiple Fluorescence Labels;

Near Super-resolution Confocal Microscopy

Pre-antibody Testing/optimization With New Antibodies.

Pre-experiment Consultation And Consultation Prior To Antibody Purchase

Widefield Microscopy

Facility Equipment

DAKO Autostainer Plus

Zeiss Axioimager M1

Zeiss LSM 880 Live Cell Confocal System with Fast Airyscan

Zeiss LSM880 Upright Platform Confocal System

No additional equipment has been listed

(To see more facility information, click the in the upper right)

Publications

Publications associated with this facility (Click To View):

1.) Chen AM, Azar SS, Harris A, Brecha NC, Pérez de Sevilla Müller L (2021). PTEN Expression Regulates Gap Junction Connectivity in the Retina. Frontiers in neuroanatomy, 15(), 629244. . PMID: 34093139.

2.) Pérez de Sevilla Müller L, Solomon A, Sheets K, Hapukino H, Rodriguez AR, Brecha NC (2019 Jan 1). Multiple cell types form the VIP amacrine cell population. The Journal of comparative neurology, 527(1), 133-158. doi: 10.1002/cne.24234. PMID: 28472856.

3.) Solomon AM, Westbrook T, Field GD, McGee AW (2018). Nogo receptor 1 is expressed by nearly all retinal ganglion cells. PloS one, 13(5), e0196565. . PMID: 29768445.

4.) Pérez de Sevilla Müller L, Azar SS, de Los Santos J, Brecha NC (2017). Prox1 Is a Marker for AII Amacrine Cells in the Mouse Retina. Frontiers in neuroanatomy, 11(), 39. . PMID: 28529477.

5.) Vujovic N, Gooley JJ, Jhou TC, Saper CB (2015 Dec 15). Projections from the subparaventricular zone define four channels of output from the circadian timing system. The Journal of comparative neurology, 523(18), 2714-37. . PMID: 26010698.

6.) Pérez de Sevilla Müller L, Sargoy A, Fernández-Sánchez L, Rodriguez A, Liu J, Cuenca N, Brecha N (2015 Jul 1). Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina. The Journal of comparative neurology, 523(10), 1443-60. . PMID: 25631988.

7.) Liu X, Hein J, Richardson SC, Basse PH, Toptan T, Moore PS, Gjoerup OV, Chang Y (2011 May 13). Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus. The Journal of biological chemistry, 286(19), 17079-90. . PMID: 21454559.

(To see more facility information, click the in the upper right)

Associations

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Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=3091
https://coremarketplace.org/RRID:SCR_012312

Institution

Institution ROR ID: https://ror.org/04drvxt59

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

immunostaining services, image processing services, confocal microscopy, light microscopy, fluorescence microscopy, brightfieqld microscopy, immunoperoxidase staining, immunofluorescence staining

Resource Type:

access service resource, core facility, service resource

Citation:

Beth Israel Deaconess Medical Center Confocal Imaging Core Facility (RRID:SCR_012312)

BIDMC-Immunostaining and Microscopy Core (Microscopy (Electron, Fluorescence, Optical))

Quicklinks:

Facility LIMS Page

Primary Contact:

Facility RRID

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Facility Profile QR Code (links to this profile)

(To see more facility information, click the in the upper right)

Contact Hagen, Susan J

Contact The CoreMarketplace

BIDMC-Immunostaining and Microscopy Core

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