The CoreMarketplace: Harvard Center for Biological Imaging (HCBI)

The Harvard Center for Biological Imaging was opened May 17, 2010 and is one of the most innovative biological imaging centers in the world. In order to ensure investigators always have access to the latest and best technologies, the HCBI was designed to be an “Evergreen” microscopy facility. Imaging systems at the HCBI are leased rather than purchased and replaced every 2-3 years. This ensures that the facility remains relevant and is never in possession of outdated equipment. Currently, the HCBI contains 20 microscopes and 12 high-end processing stations including confocal, super-resolution, multi-photon, lightsheet, live cell imaging, high-content screening, slide scanning, spatial transcriptomics, laser dissection and macroscopy. The facility also features wet lab space, eukaryotic and prokaryotic incubation, and access to nearby animal care facilities for a wide range of species.

Facility Policies

Services are offerred outside of Harvard University

Consulting is offerred outside of Harvard University

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Services offered:

Analysis Workstations

Cell Imaging

Confocal Microscope With FCS/FLIM Capabilities

Confocal Microscopy

Confocal/ Super Resolution Microscope

Consultations

Data Analysis

Data Processing And Interpretation

Differential Interference Contrast Microscopy

Fluorescence Lifetime

Fluorescence Lifetime Imaging

Fluorescence Microscope With Optical Sectioning Capability

Forster Resonance Energy Transfer (FRET)

Freeze Substitution

High Content Screening

Laser Capture Microdissection

Laser Scanning

Light-sheet Microscopy

Live Cell Imaging

Microscopy

Multiphoton Microscopy

Multiphoton/ Harmonic Generation Microscope

Multiplex Fluorescence

Multiplexed Immunofluorescence

Optical Imaging

Phase Contrast Microscopy

Photomanipulation

Scanning 20X Brightfield

Scanning 20X Flourescent

Scanning 20X Fluorescent

Scanning 40X Brightfield

Scanning 40X Fluorescent

Single-molecule Imaging

Slide Scanning

Spinning Disk Confocal Imaging

STED Nanoscopy

Stereomicroscopy

Super-resolution Microscopy

TIRF Microscopy

Training

Whole Slide Imaging

Facility Equipment

Bruker TruLive

Lightsheet microscope for aqueous samples only. 12.5x, 25x, 37.5x, and 50x magnifications available

Logos X-Clarity

Equipment for electrophoretic tissue clearing

Nikon Eclipse Ti2 with CSU-W1 Spinning Disk

Inverted microscope with Yokogawa spinning disk and dual Prime BSI cameras

Yokogawa CV8000

High content screening microscope for multi-well plates

Zeiss Axio Zoom.V16

Stereomicroscope with brightfield, polarization, and fluorescence imaging capabilities

Zeiss Axioscan 7

Digital Slide Scanner that holds up to 100 slides at a time

Zeiss ELYRA 7

Super-resolution microscope with TIRF, HILO, SIM, and SMLM (PALM/STORM) capabilities

Zeiss Lightsheet 7

Lightsheet microscope with 2.5x, 5x, 10x, and 20x capabilities

Zeiss LSM 700

Laser Scanning Confocal microscope with laser lines 405, 488, 561, 638

Zeiss LSM 880 with FLIM

Laser scanning confocal microscope with Picoquant FLIM capabilities

Zeiss LSM 900

Laser scanning confocal microscope

Zeiss LSM 900 Materials

Upright Laser Scanning Confocal microscope with DIC, Phase contrast, Polarization, Reflected light and Fluorescence Imaging

Zeiss LSM 980 NLO

Upright scanning confocal laser with tunable IR laser and 4 NDDs for two-photon microscopy (2P_

Zeiss LSM 980 with Airyscan

Laser scanning confocal microscope with airyscan dector and NIR detector

Zeiss PALM Laser Microdissection Microscope

Microscope for laser microdissection of thin tissue sections

https://hcbi.fas.harvard.edu/equipment

(To see more facility information, click the in the upper right)

Publications

Publications associated with this facility (Click To View):

1.) Ludin A, Stirtz GL, Tal A, Nirmal AJ, Besson N, Jones SM, Pfaff KL, Manos M, Liu S, Barrera I, Gong Q, Rodrigues CP, Sahu A, Jerison E, Alessi JV, Ricciuti B, Richardson DS, Weiss JD, Moreau HM, Stanhope ME, Afeyan AB, Sefton J, McCall WD, Formato E, Yang S, Zhou Y, van Konijnenburg DP, Cole HL, Cordova M, Deng L, Rajadhyaksha M, Quake SR, Awad MM, Chen F, Sorger PK, Hodi FS, Rodig SJ, Murphy GF, Zon LI (2024 Sep 19). Craters on the melanoma surface facilitate tumor-immune interactions and demonstrate pathologic response to checkpoint blockade in humans bioRxiv, (), 2024.09.18.613595. . PMCID: 11429731.

2.) Han X, Lu X, Li PH, Wang S, Schalek R, Meirovitch Y, Lin Z, Adhinarta J, Murray KD, MacNiven LM, Berger DR, Wu Y, Fang T, Meral ES, Asraf S, Ploegh H, Pfister H, Wei D, Jain V, Trimmer JS, Lichtman JW (2024 Aug 5). Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex Nature Communications, 15(), 6648. . PMCID: 11300613.

3.) Noonan HR, Thornock AM, Barbano J, Xifaras ME, Baron CS, Yang S, Koczirka K, McConnell AM, Zon LI (2024 Jun 14). A chronic signaling TGFb zebrafish reporter identifies immune response in melanoma eLife, 13(), e83527. . PMCID: 11178360.

4.) Davis JC, Ryaboshapkina M, Kenty JH, Eser PÖ, Menon S, Tyrberg B, Melton DA (2024 Apr 11). IAPP Marks Mono-hormonal Stem-cell Derived β Cells that Maintain Stable Insulin Production in vitro and in vivo bioRxiv, (), 2024.04.10.587726. . PMCID: 11030367.

5.) Pearce S, Lin C, Pérez-Mercader J (2024 Feb 19). Adaptive and Dissipative Hierarchical Population Crowding of Synthetic Protocells through Click-PISA under Gradient Energy Inputs Nano Letters, 24(8), 2457-2464. . PMCID: 10906081.

6.) Lowet AS, Zheng Q, Meng M, Matias S, Drugowitsch J, Uchida N (2024 Jan 3). An opponent striatal circuit for distributional reinforcement learning bioRxiv, (), 2024.01.02.573966. . PMCID: 10802299.

7.) Zhang A, Zwang TJ, Lieber CM (2023 Nov 29). Biochemically functionalized probes for cell-type–specific targeting and recording in the brain Science Advances, 9(48), eadk1050. . PMCID: 10686571.

8.) Han X, Li PH, Wang S, Sanchez M, Aggarwal S, Blakely T, Schalek R, Meirovitch Y, Lin Z, Berger D, Wu Y, Aly F, Bay S, Delatour B, LaFaye P, Pfister H, Wei D, Jain V, Ploegh H, Lichtman J (2023 Nov 1). A large-scale volumetric correlated light and electron microscopy study localizes Alzheimer's disease-related molecules in the hippocampus. bioRxiv : the preprint server for biology, (), . . PMID: 37961104.

9.) Zhang A, Zwang TJ, Lieber CM (2023 Oct 3). Biochemically-functionalized probes for cell type-specific targeting and recording in the brain. bioRxiv : the preprint server for biology, (), . . PMID: 37873102.

10.) He LS, Qi Y, Allard CA, Valencia-Montoya WA, Krueger SP, Weir K, Seminara A, Bellono NW (2023 Sep 26). Molecular tuning of sea anemone stinging bioRxiv, (), 2023.06.15.545144. . PMCID: 10418081.

11.) Lafuente-Gómez N, de Lázaro I, Dhanjani M, García-Soriano D, Sobral MC, Salas G, Mooney DJ, Somoza Á (2023 Sep 24). Multifunctional magnetic nanoparticles elicit anti-tumor immunity in a mouse melanoma model Materials Today Bio, 23(), 100817. . PMCID: 10562177.

12.) Han X, Lu X, Li PH, Wang S, Schalek R, Meirovitch Y, Lin Z, Adhinarta J, Berger D, Wu Y, Fang T, Meral ES, Asraf S, Ploegh H, Pfister H, Wei D, Jain V, Trimmer JS, Lichtman JW (2023 Jul 6). Multiplexed volumetric CLEM enabled by antibody derivatives provides new insights into the cytology of the mouse cerebellar cortex Research Square, (), rs.3.rs-3121892. . PMCID: 10350204.

13.) Tian H, Davis HC, Wong-Campos JD, Park P, Fan LZ, Gmeiner B, Begum S, Werley CA, Borja GB, Upadhyay H, Shah H, Jacques J, Qi Y, Parot V, Deisseroth K, Cohen AE (2023 Jul). Video-based pooled screening yields improved far-red genetically encoded voltage indicators. Nature methods, 20(7), 1082-1094. . PMID: 36624211.

14.) Spitzberg JD, Ferguson S, Yang KS, Peterson HM, Carlson JC, Weissleder R (2023 Mar 4). Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact Nature Communications, 14(), 1239. . PMCID: 9985597.

15.) Tian H, Davis HC, Wong-Campos JD, Park P, Fan LZ, Gmeiner B, Begum S, Werley CA, Borja GB, Upadhyay H, Shah H, Jacques J, Qi Y, Parot V, Deisseroth K, Cohen AE (2023 Jan 9). Video-based pooled screen yields improved far-red genetically encoded voltage indicators Nature methods, 20(7), 1082-1094. . PMCID: 10329731.

16.) Guerra San Juan I, Nash LA, Smith KS, Leyton-Jaimes MF, Qian M, Klim JR, Limone F, Dorr AB, Couto A, Pintacuda G, Joseph BJ, Whisenant DE, Noble C, Melnik V, Potter D, Holmes A, Burberry A, Verhage M, Eggan K (2022 May 18). Loss of mouse Stmn2 function causes motor neuropathy. Neuron, 110(10), 1671-1688.e6. . PMID: 35294901.

17.) Ghosh S, Leng W, Wilsch-Bräuninger M, Barrera-Velázquez M, Léopold P, Eaton S (2022 Apr 25). A local insulin reservoir in Drosophila alpha cell homologs ensures developmental progression under nutrient shortage. Current biology : CB, 32(8), 1788-1797.e5. doi: 10.1016/j.cub.2022.02.068. PMID: 35316653.

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Associations

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Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=981
https://coremarketplace.org/RRID:SCR_018673

Institution

Institution ROR ID: https://ror.org/03vek6s52

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, microscopy, cell imaging, confocal microscopy, consultation, data analysis, data processing, data interpretation, laser capture microdissection, microscopy, multiphoton microscopy, optical imaging, slide scanning, whole slide imaging, ABRF

Resource Type:

service resource, core facility, access service resource

Citation:

Harvard Center for Biological Imaging Core Facility (RRID:SCR_018673)

Harvard Center for Biological Imaging (HCBI) (Microscopy (Electron, Fluorescence, Optical))

Quicklinks:

Primary Contact:

Facility RRID

Facility Details

Facility Policies

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Services offered:

Facility Equipment

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Publications

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Associations

ABRF

NERLSCD

BINA

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Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Facility Profile QR Code (links to this profile)

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Harvard Center for Biological Imaging (HCBI)

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