The overall objective of the Molecular Genetics Core Facility is to provide high quality, low cost genomic technology services and expertise in a timely, affordable manner. Services offered include:
Nanopore PromethION sequencing
Electronic optical mapping on Nabsys
Optical Genome mapping on Bionano Saphyr
NGS (Illumina-based)
Sanger Sequencing
Single cell analysis/RNAseq
Transcriptomic spatial profiling
Gene expression analysis
Droplet Digital PCR & qPCR
Microsatellite/fragment analysis
SNP genotyping
Chromosomal microarray analysis
Sample prep & QC
Project consultation
Bioinformatics support
contact us at MGCF@childrens.harvard.edu
Facility Policies
Services are offerred outside of Boston Children's Hospital
Consulting is offerred outside of Boston Children's Hospital
The CFX384 Touch is a real-time PCR instrument in a 384-well format that performs high-throughput data acquisition. The CFX384 Touch System utilizes temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer.The CFX384 has up to four-target detection, thermal cycler, stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR. [Product Link]
The CFX96 Touch System is a real-time PCR detection system that combines optical technology with temperature control. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, thermal cycler performance, unrivaled stand-alone functionality, and easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. [Product Link]
Droplet generator that provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. [Product Link]
Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems
Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]
