New York University School of Medicine
550 First Ave.
New York, NY 10016United States of Americahttps://med.nyu.edu/research/research-resources/scientific-cores-shared-resources/rodent-genetic-engineering-laboratory
Dr. Sang Yong Kim
Last Updated: 01/02/2019
The Rodent Genetic Engineering Laboratory (RGEL) at NYU Langone Medical Center is a resource for researchers who want to generate mutant mice by gene targeting in ES cells, including those taking advantage of the CRISPR/Cas9 system. Among the services we offer to the NYULMC research and external research community are the production of transgenic mice by pronuclear injection of plasmid DNA or BAC DNA and the production of ES cell chimeras by blastocyst injection, production of fully ES-cell derived mice (ES-mice) by tetraploid complementation, as well as the production of genetically modified mice by CRISPR/Cas9 â€“mediated genome engineering. In additional, the core offers sperm and embryo cryopreservation services to our users to save time, money and mouse space by freezing their valuable strains. RGEL also provides IVF services using fresh or cryopreserved sperm samples. Moreover, RGEL imports fresh and/or frozen embryos for use in rederivationsâ€”this effort saves investigators time and additional costs by eliminating the need for quarantining their imported animals. Gene targeting services are also offered through RGEL. In addition to targeting any ES cell line of choice (electroporation, selection and cryopreservation of ES cell clones), RGEL can thaw and expand previously targeted clones for injection.
RGEL lab scientists customize numerous advanced technologies to meet individual project needs, including the following:
â€¢ CRISPR/Cas9 Genome Editing
â€¢ Transgenic Mouse
â€¢ Gene Targeting in Mouse ES Cells
â€¢ A proprietary rapid strain-creation method known as the EZ-Mouse Model.
â€¢ Tetraploid complementation systems
â€¢ IPS Cell Tetraploid Microinjection
â€¢ ES Cell Blastocyst Microinjection
â€¢ GEMMs-ESC Speedy Models, regulated gene expression by high-throughput RNA interference assay.
â€¢ Embryo and Sperm Cryopreservation
â€¢ In Vitro Fertilization (IVF)
â€¢ TARGATTâ„¢ Site Specific Knock-in Mice
â€¢ ESC/iPS Cell Karyotyping
â€¢ Coordination of Imports and Exports.
Services are offerred outside of New York University School of Medicine
Consulting is offerred outside of New York University School of Medicine
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