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Antibody & Bioresource Core Facility (Antibody Development)

Memorial Sloan Kettering Cancer Center

417 E 68th Street - ZRC 1553

New York City, NY 10065

United States of America

https://www.mskcc.org/research/ski/core-facilities/monoclonal-antibody-core-facility

Primary Contact:

Frances Weis-Garcia/ Ph.D.

Last Updated: 04/21/2021

Facility RRID

RRID:SCR_017691

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Facility Grant Number

2 P30 CA008748-53

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Facility Details

The Antibody and Bioresource Core Facility supports researchers at both Memorial Sloan-Kettering Cancer Center and The Rockefeller University by making it easier for them to:


1.) Develop new monoclonal antibodies that meet their experimental needs through any combination of these services:

... Consultation
... Immunization of mice, rats and Armenian hamsters
... Electrofusion (B cell immortalization / generation of 10,000's of hybridoma)
... Isolation of IgG secreting hybridomas, which is 1 - 20% of the hybridomas, for screening to enable a greater number of hybridomas are screened
... ELISA screening
... Cryopreservation and storage of positive cultures
... Subcloning
... Cryopreservation and storage of unscreened fusion products / hybrridomas for future screening


2.) Produce monoclonal antibodies

... Conditioned Media
...... 1 to 10 ug monoclonal antibody / ml

... Bioreactor Supernatants:
...... 0.5 to 4 mg monoclonal antibody / ml
...... Purity ranges from 20 to 80%

... Purified monoclonal antibodies:
...... 1 to 10 mg monoclonal antibody / ml
...... Purity > 95%

... Conjugations

... Fragmentation


3.) Mycoplasma Testing


4.) Obtain cell lines developed at Memorial Sloan Kettering Cancer Center and The Rockefeller University


This facility is designed to be both an informational resource as well as a group that provides services for a fee.

Depending upon internal demand, external requests are welcome.

Facility Policies

Services are offerred outside of Memorial Sloan Kettering Cancer Center

Consulting is offerred outside of Memorial Sloan Kettering Cancer Center