Sanford-Burnham Medical Research Institute
10901 North Torrey Pines Road
La Jolla, CA 92037United Stateshttp://sanfordburnham.org/technology/sr/Pages/LaJolla_Proteomics.aspx
Alex Rosa Campos
Last Updated: 02/06/2014
SBMRI Proteomics Facility provides state-of-the-art mass spectrometry (MS)-based proteomic services for analysis of peptides, synthetic compounds, carbohydrates and proteins by facilitating protein identification, the qualitative and quantitative characterization of post-translational modifications, plus quantitative and differential proteomic profiling of low-complex biological samples. Facility staff regularly identifies, validates, develops, and implements new methodologies for identification, quantification and characterization of biologically important proteins. Such approaches can support a mechanistic understanding of cancer and other life threatening diseases, as well as identify new therapeutic targets and diagnostic biomarkers.
The Proteomics Facility also supports users with grant write-up, proteomics software applications, data analysis, and experimental design of proteomic studies. The facility is continuously expanding its capabilities in large-scale differential proteomics analysis and in phosphoproteomics (and other PTMs) for discovery of potential biomarkers and mapping protein profiles.
ïƒ˜ Molecular Weight (MW) Determination MW of peptides and protein and protein/Peptide cleavage mapping
ïƒ˜ Protein Identification from 1D or 2D gels, IP enriched material, simple â€“complex sample
ïƒ˜ Characterization of post-translational modifications like phosphorylation, ubiquitination, methylation etc
ïƒ˜ Comparative and Quantitative proteomics analysis of simple to complex biological samples using Mass-Tagged or Label-Free approach
ïƒ˜ Analysis tools for CID and ETD include Sage-N SORCERER Enterprise platform, Integrated Proteomics Pipeline (IP2 from Yates lab) utilizing cloud computing and MaxQuant on a local server using MASCOT
ïƒ˜ Hands on Training on MALDI-TOF/TOF
Services are offerred outside of Sanford-Burnham Medical Research Institute
Consulting is offerred outside of Sanford-Burnham Medical Research Institute