Sanford-Burnham Medical Research Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037United Stateshttp://www.sanfordburnham.org/technology/sr/Pages/LaJolla_ProteinProductionandAnalysis.aspx
Andrey Bobkov, Ph.D.
Last Updated: 08/20/2012
Variety of protein production services including cloning, expression optimization, development of protein purification protocols, and protein preparation. E. coli, insect (SF9, baculovirus) and mammalian (293) systems are available for protein expression. Our analytical services are focused on biophysical characterization of structural and functional properties of proteins in solution, under native, non-denaturing conditions. We can perform quality control of protein samples (folding, stability, aggregation, etc.); characterize protein conformation and shape in solution; and measure protein binding to proteins, peptides, small molecules and other ligands (including determination of equilibrium (Kd) and kinetic rate (kon, koff) constants, stoichiometry, binding enthalpy and entropy).
- Protein Production
*Protein expression trials
*Optimization of protein purification
*Cell pellets/conditioned media production
- Protein Analysis
*Analytical ultracentrifugation (AUC)
*Atomic force microscopy
*Differential scanning calorimetry (DSC)
*Fast kinetics, stopped flow
*FPLC, analytical runs
*Isothermal titration calorimetry (ITC)
Services are offerred outside of Sanford-Burnham Medical Research Institute
Consulting is offerred outside of Sanford-Burnham Medical Research Institute