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Sanford-Burnham Medical Research Institute
10901 N. Torrey Pines
La Jolla, CA 92037
United States of Americahttp://www.sanfordburnham.org/technology/sr/Pages/LaJolla_Histopathology.aspxLast Updated: 08/20/2012
The Histopathology Core Facility provides standard and customized research specific histology services including tissue processing, fixed and frozen sectioning. The Facility also offers routine and special histological stains as well as immunohistochemistry and immunofluorescence. The Facility maintains and provides training for several pieces of common use equipment, such as, paraffin microtome, cryostat, and a cytospin. Training is also provided for basic histological techniques such as, perfusion and tissue collection. Finished slides are returned to the researcher for evaluation, or they may be scanned in our Aperio Scanscopes that allows digitizing of slides for archiving, and electronic distribution/remote analysis of histology slides, and offer, powerful image analysis tools. Scanning of fluorescent slides is also available.
Conventional histology
- Routine and custom sectioning
- Paraffin and frozen samples
- Standard and special stain (H&E, PAS, silver impregnation)
Immunohistochemistry
- Development of custom protocols (overlay assays, competition assays)
- Titration and staining of commercial and custom antibodies
- Double labeling of antibodies
- Immunofluorescence
Training
Services are offerred outside of Sanford-Burnham Medical Research Institute
Consulting is offerred outside of Sanford-Burnham Medical Research Institute
- Leica CM 3050 cryostat
- Leica RM 2125 paraffin microtome
- Dako autostainer plus
- Shandon Cytospin 3
- Sakura Tissue Tek vacuum infiltration tissue processor
- Leica EG 1160 paraffin embedding station
- MMI Cell Cut Laser Microdissection system
- Aperio Scanscopes FL and XT systems
No publications have been associated with this facility
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