Sanford-Burnham Medical Research Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
United States of Americahttp://www.sanfordburnham.org/technology/sr/Pages/LaJolla_GenomicsMicroarrayQPCR.aspxcite this facility
Kang Liu/ Ph.D.
Last Updated: 08/20/2012
The facility provides microarray-based global analysis of gene expression in human, mice and rats using an Illumina platform. Starting with investigator RNA samples, the facility confirms RNA quality, and then performs microarray analysis as well as initial data analysis. Array data are returned to the investigator, and if desired, also passed on to the Informatics and Data Management facility for more detailed analysis. Both mRNA and miRNA profiling are available. Quantitative real-time PCR (Q-PCR) service is predominantly SYBR green-based analysis, but hybridization probe (e.g. TaqMan) approaches are supported. Starting with RNA, the facility will utilize primers from investigators, or design custom primers and perform Q-PCR. The facility provides control primers and assists with experimental design, as well as providing basic relative quantitation analysis. Q-PCR analysis in the facility can also provide gene, RNA, or viral copy number data.
Services
RNA sample quality analysis
Microarray analysis: from total RNA to data for global mRNA or miRNA expression
Initial microarray data analysis, with further informatic analysis available through the Bioinformatics facility
Q-PCR service: from total RNA to data analysis – one step from RNA, or first synthesizing cDNA (2 step)
SYBR Green or Hybridization Probes (predominantly SYBR Green)
Special services: RNA purification and Q-PCR Primer design
Services are offerred outside of Sanford-Burnham Medical Research Institute
Consulting is offerred outside of Sanford-Burnham Medical Research Institute
