Facility Details

The Genomics Core provides resources to BRI and external investigators for genomics analysis, from sample processing through data generation, as well as basic bioinformatics analysis, provided by our Bioinformatics Core Team. Currently available technologies focus on Illumina RNA and DNA sequencing. RNA-seq can be performed from a variety of sample types, including purified total RNA, cell populations (including low input samples of 50-1000 cells), or single cells (either plate-sorted rare populations or high-throughput 10x single-cell analyses). DNA-seq pipelines can also be performed, including bulk CUT&Tag/CUT&RUN, and ATAC-seq and single cell ATACseq and multiome capture. Custom prepared libraries can also be submitted for sequencing.

The Core laboratory houses three Illumina sequencers, two NextSeq2000 instruments and a Miseq, plus a 10x Chromium X and a 10x CytAssist. The Chromium X is able to run GEM-X assays on fresh cells and Flex assays on fixed cells. The CytAssist works to perform spatial profiling using Visium HD technology.

Additional equipment includes a ThermoFisher Kingfisher Flex, for extracting nucleic acid from a variety of sample types using magnetic bead technology with a throughput of up to 96 samples. Two Qiagen QIAcube Connect instruments automate extraction of nucleic acid using spin column technology. Ancillary equipment for sample processing includes a Formulatrix Mantis Liquid Handler, an Agilent TapeStation 4200 (to measure RNA/DNA integrity and size), and a Tecan NanoQuant (for high throughput sample quantitation).