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Rockefeller University
1230 York Avenue
Box 203
New York, NY 10065
United Stateshttp://www.rockefeller.edu/htsrc/indexFraser Glickman
Last Updated: 03/05/2021
The High-Throughput Screening Resource Center (HTSRC) supports scientists in the screening and identification of compounds and genetic modulators of in vitro assays which utilize optical and other bio-analytical technologies. The center has a collection of 169,000 compounds, automated liquid transfer devices, compound databases, and supports a broad diversity of assay development techniques, typically found in early drug discovery programs. The High- Throughput Screening Resource Center also oversees the management of, training for and scheduling for optical spectroscopic and calorimetric equipment for use in studies of the structure, function and interactions of biological and organic molecules.
The center is staffed full-time under an open-access model, whereby, once trained, investigators can use the facility 24/7. The staff includes a director with over 20 years of screening and drug discovery experience in the pharmaceutical industry, who is readily available for scientific and technical consultations, and three research associates, who support the automation, data processing, liquid handling and instrument QC and maintenance. The Center has the capacity to support approximately 15 full high throughput screening (HTS) projects per year, in addition to various pre-HTS and post-HTS activities.
The center is directed by J. Fraser Glickman who has a Ph.D. in biochemistry from UC Santa Barbara and over two decades of experience in drug discovery and HTS including a decade working in the pharmaceutical industry. Dr. Glickman has been directing the HTSRC since 2008.
The center occupies 1500 sq. ft. of lab space located on the Rockefeller campus on the Upper East Side of Manhattan in proximity to Weill-Cornell Medical College, Memorial-Sloan Kettering Cancer Center and the NYU Langone Medical Center. The lab space is equipped with assay development workbenches and data analysis workstations, analytical instruments, electronic automated pipetters and houses a full cell culture facility. Cell dispensing can be done under full laminar flow biosafety cabinets, and the center is certified for work at BSL2.
Services are offerred outside of Rockefeller University
Consulting is offerred outside of Rockefeller University
The Center is configured for processing 96, 384 and 1536-well microtiter plates through the use
of automated changeable tip dispensers, non-contact dispensers, and syringe dispensers. For assay technologies, the HTSRC has the capability to support cellular and biochemical assays using
absorbance, fluorescent kinetics, fluorescence anisotropy, time-resolved fluorescence,
time-resolved fluorescence resonance energy transfer, AlphaScreen, bioluminescence, for example luciferase and green fluorescent protein, scintillation proximity and cellular fluorescence imaging. Assay targets can include ion channel receptors, enzymes, protein interactions, signaling pathways and cellular processes.
Plate Readers
Perkin-Elmer Envision fluorescence polarization, time gated fluorescence, prompt fluorescence luminescence, alphascreen 384 wells / 2 minutes
Hammmatsu FDSS 6000 Fluorescence Kinetics, calcium flux assays 384 wells in 2-4 minutes
Flexstation II Dual Monochromator
Fluorescence Kinetics, calcium flux assays
96 wells in 2-4 minutes Perkin-ElmerTriLux (Scintillation and Luminescence) 384 wells in 30-40 minutes scintillation 384 wells in 5 minutes luminescence Molecular Devices ImageXpress Micro Fluorescence Microscopic Imaging
384 wells in 15-40 minutes fed with a Thermo CRS robot arm driven by POLARA scheduling software.
MetaXpress software can analyze and score imaging for morphological and subcellular events, such as translocation, spot formation and process outgrowth. The detection system is based on a fluorescence microscope with automatic laser focusing. Molecular Devices Analyst GT fluorescence polarization, time gated fluorescence, prompt fluorescence, luminescence
384 wells in 2 minutes
Molecular Devices Image Express Velo Laser Scanning Cytometer
This two color (488nm and 534nm) laser-based scanning imager can image 384 wells in 5-10 minutes.
The system is equipped with a Twister II robot arm such that 40 plates can be processed unattended.
The software can score fluorescent intensity per cell , number of cells, surface area of cells,
ellipticity of cells in up two three individual emission bands. Biotek synergy H4 with Biostack
Plate Feeder
A multi-purpose high-speed microplate reader with dual monochromators which can allow the user to dial in the particular wavelengths of interest. Can read alphaLisa, TR-FRET, assays. Equipped with in-line injection port which allows for rapid kinetic analyses.
Analytical Instruments
The following instruments and associated techniques are available: a Fourier transform infrared spectrometer (FTIR), a circular dichroism spectrometer (CD), a surface plasmon resonance (Biacore 3000) detector (SPR), a scanning spectrofluorometer and an isothermal calorimeter (GE AutoITC 200).
Fluidics and Automated Pipetters
Perkin-Elmer Janus Automated Workstation
Disposable tips or nano head syringes are used for compound dispensing in the 50 nL-50 uL
range, 96, 384, or 1536-well formats
Perkin-Elmer Janus Mini Disposable tips or nano head syringes 50 nL-20 uL, 96 or 384-well formats Thermo MultiDrop Combi with 50 plate feeders. This is a Non-contact, peristaltic plate filler capable of dispensing 1uL-200uL of solution or suspension in 96, 384, or 1536 well formats with high precision (CV <5%) . 50 plates can be loaded onto the stacker, and fed with a flask of single cell suspension, in 50 minutes. The peristaltic tubing system used to control the volume dispensed has no effect on cell viability in microtiter plates. The Multi-drop combi is housed in a Biosero BigNeat Laminar flow HEPA- filtered biosafety cabinet such that sterility and safety is maintained. The replaceable tubing cassette is completely autoclavable.
Union Biometrica COPASâ„¢ Select
For sorting large particles into microtiter plates
Biotek EL406 Microtiter Plate Washer with BioStack automated plate feeder Washes a 96/384 well plate in 30 seconds or less, with precise control of flow rates and tip
distances.
SeahorseXFe96 Analyzer
NanoTemper MST monolith 115-a microscale thermophoresis instrument that allows for detection of conformational changes in molecules and is well-suited for measuring equilibrium binding affinities. The technique requires the presence of a fluorophore and can be run with small analyte volumes
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Citation:
Rockefeller University High Throughput Screening Resource Center Core Facility (RRID:SCR_020985)
Facility Details
About This Facility
Services and Equipment
Publications
Associations
Metadata
