Facility Details

The Rodent Genetic Engineering Laboratory (RGEL) at NYU Langone Medical applies advanced molecular genetic techniques in rodent models. Experienced at generating novel mutant mouse strains by using gene targeting in embryonic stem cells (ESCs) and by developing novel methods of modifying rodent genomes and assaying genetically modified cells in vivo. Customizes technologies to meet individual project needs, including specialized expertise in use clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) systems and other approaches for direct genome editing in vivo.Services include CRISPR/Cas9,proprietary rapid strain-creation method known as EZ-Mouse Model, tetraploid complementation systems, GEMMs-ESC Speedy Models, regulated gene expression by high-throughput RNA interference assay in mice, safe-harbor knock-in gene editing using Rosa26 and other loci, recombinase-mediated cassette exchange, Inducible Tet-On and Tet-Off Gene Expression Systems, homologous recombination, induced pluripotent stem cells, assisted reproductive technologies, and vitrification systems,production of transgenic mice by pronuclear injection of plasmid DNA or bacterial artificial chromosome DNA and the production of ESC chimeras by blastocyst injection. Production of fully ESC-derived mice by tetraploid complementation, including following, isolating, and manipulating novel ESC lines from existing mouse strains.Offers sperm and embryo cryopreservation and storage technologies for strain preservation and recovery. Highly efficient in vitro fertilization technologies using fresh or cryopreserved sperm are available, facilitating import of novel mouse strains without quarantine and recovery of poorly breeding strains.Offers traditional embryo rederivation technologies for animal import into barrier vivaria through quarantine.