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1230 York Avenue
New York, NY 10065United States of Americahttps://www.rockefeller.edu/fcrc/
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Last Updated: 09/11/2023
State-of-the-art Flow Cytometry Resource Center. Cell sorting is performed for extended hours by staff. Analysis and cell sorting are offered as self service with 24/7/365 access to the fully trained users.
Services are offerred outside of Rockefeller University
Consulting is not offerred outside of Rockefeller University
BD FACSAria IIu High Speed Cell Sorter
Aria has four lasers, 405, 488, 561 and 633 nm, and can detect light in sixteen photomultiplier tubes. Its primary function is to examine complex populations of cells and yield pure populations of cells. The Aria can sort lymphocytes at a rate of approximately 15,000 events per second. For larger cells, the rate is much slower in order to optimize the passage of cells through the instrument. Cells from single populations can be sorted into 96-well plates. Alternatively, the Aria can sort into two 15-ml tubes or four 5-ml tubes. The Aria can purify samples that are simply positive and negative for a single fluorophore or as complex as nine-color samples with intricate gating schemes. The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 405 nm, 488 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 ?m, 85 ?m and 100 ?m nozzles. If you need a yellow-green (561 nm) laser, check the BD FACSAria III instead. [Product Link]
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BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
Citation IDs: Center for AIDS Research award number 5P30AI050410
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Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
Citation IDs: RRID:SCR_017788
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Agilent NovoCyte Penteon
Conventional Flow Cytometer with 5 lasers/30 channels and NovoSampler Q. It provides an expanded set of capabilities that accommodate today’s high-end and increasingly sophisticated multi-color flow cytometry assays. Researcher has flexibility to choose from up to 30 fluorescence channels utilizing up to 5 lasers with up to 30 independent detectors. The NovoSampler Q can be integrated into different laboratory automation platforms and efficiently processes both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. The intuitive and industry leading NovoExpress software has been further advanced, providing an exceptional user experience in data acquisition, analysis and reporting. https://www.agilent.com/en/product/research-flow-cytometry/flow-cytometers/flow-cytometer-systems/novocyte-penteon-flow-cytometer-1270342
Sony MA900 Cell Sorter
4 lasers/12 channels. The MA900 from Sony is developed with powerful, modern technologies including a patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability. Choose from four excitation lasers — 488 nm, 405 nm, 561 nm, and 638 nm — on two beam spots, the MA900 supports choice and flexibility, enabling the detection of fluorescence signals up to 12 fluorescence parameters and two scatter parameters. With the highest level of automation available on any cell sorter, the MA900 uses sensors, software, and engineering know-how across the workflow from startup to aseptic cleaning, QC, and sort setup. Intelligent automation dramatically simplifies operation and streamlines troubleshooting. The MA900 offers 70 μm and 100 μm nozzle sizes that meet the needs of most sorting applications. Once events are gated on plots, target cells can be sorted for further analysis. A choice of eight default and five custom sort modes is available to achieve the desired purity and yield of the sorted population. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 24-, 48-, 96-, and 384-well plates. The MA900 is installed inside an custom class A2 level II biosafety cabinet, which incorporates a built-in aerosol management system that operates independently to actively evacuate aerosols from the sort collection chamber. The custom biosafety cabinet is designed and tested to meet international standards including the National Sanitation Foundation Standard 49 (NSF49). For greater details please go to https://www.sonybiotechnology.com/us/instruments/ma900-multi-application-cell-sorter/
ThermoFisher Attune™ NxT Flow Cytometer
4 lasers/14 channels. Attune NxT was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows to run samples up to 10X faster and achieve greater resolution with little fear of sample loss due to clogging, for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality. The addition of the CytKick™ Max Autosampler enables significantly faster high-throughput screening on a multiparametric platform in plate formats, including 96-well, 384-well, and deep-wells with the high volume intake. The Attune NxT flow cytometer is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, microbiological assays, and more. https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/attune-nxt-flow-cytometer.html
Publications associated with this facility (Click To View):
1.) Stewart KS, Gonzales KA, Yuan S, Tierney MT, Bonny AR, Yang Y, Infarinato NR, Cowley CJ, Levorse JM, Pasolli HA, Ghosh S, Rothlin CV, Fuchs E (2023 May 22). Stem cells tightly regulate dead cell clearance to maintain tissue fitness bioRxiv, (), 2023.05.22.541773. . PMCID: 10245816.
2.) Ogishi M, Yang R, Rodriguez R, Golec DP, Martin E, Philippot Q, Bohlen J, Pelham SJ, Arias AA, Khan T, Ata M, Al Ali F, Rozenberg F, Kong XF, Chrabieh M, Laine C, Lei WT, Han JE, Seeleuthner Y, Kaul Z, Jouanguy E, Béziat V, Youssefian L, Vahidnezhad H, Rao VK, Neven B, Fieschi C, Mansouri D, Shahrooei M, Pekcan S, Alkan G, Emiroğlu M, Tokgöz H, Uitto J, Hauck F, Bustamante J, Abel L, Keles S, Parvaneh N, Marr N, Schwartzberg PL, Latour S, Casanova JL, Boisson-Dupuis S (2023 Jan 2). Inherited human ITK deficiency impairs IFN-γ immunity and underlies tuberculosis. The Journal of experimental medicine, 220(1), . . PMID: 36326697.
3.) Ogishi M, Yang R, Rodriguez R, Golec DP, Martin E, Philippot Q, Bohlen J, Pelham SJ, Arias AA, Khan T, Ata M, Al Ali F, Rozenberg F, Kong XF, Chrabieh M, Laine C, Lei WT, Han JE, Seeleuthner Y, Kaul Z, Jouanguy E, Béziat V, Youssefian L, Vahidnezhad H, Rao VK, Neven B, Fieschi C, Mansouri D, Shahrooei M, Pekcan S, Alkan G, Emiroğlu M, Tokgöz H, Uitto J, Hauck F, Bustamante J, Abel L, Keles S, Parvaneh N, Marr N, Schwartzberg PL, Latour S, Casanova JL, Boisson-Dupuis S (2022 Nov 3). Inherited human ITK deficiency impairs IFN-γ immunity and underlies tuberculosis The Journal of Experimental Medicine, 220(1), e20220484. . PMCID: 9641312.
4.) Biegler MT, Fedrigo O, Collier P, Mountcastle J, Haase B, Tilgner HU, Jarvis ED (2022 Mar 14). Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9. Scientific reports, 12(1), 4369. . PMID: 35288582.
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