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All Facilities >> Geisel School of Medicine at Dartmouth >> Biorepository Core
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Dartmouth Medical School
1 Medical Center Drive
HD 7927, Rubin 7
Lebanon, NH 03756
http://sites.dartmouth.edu/molecepi/Tom Palys
Last Updated: 09/02/2016
The Center for Molecular Epidemiology Biorepository Core of the Geisel School of Medicine at Dartmouth utilizes a combination of manual and advanced robotic liquid handing processes, as well as traditional and state-of-the art automated archival storage strategies (e.g. ambient temperature, 4°C, -20°C, -80°C, and liquid nitrogen) to provide standardized services to the to members of the research community. The Biorepository manages a broad range of adult, child and infant specimen types, such as blood and its components, urine, nails, meconium, stool, placenta, breastmilk, DNA and RNA, as well as various environmental samples. Specimen processing services include blood fractionation, lymphocyte isolation, aliquoting of specimens for archival storage, human and microbiome nucleic acid extraction, quantification and normalization, tissue separation and fixation, specimen retrieval, shipping and referral management services. The Biorepository Core also plays a critical role in the development of new projects by working with investigators to pilot and establish methods for collection, processing, archiving and subsequent referral of study specimens. The Biorepository has processed roughly over 21,000 adult and infant specimens and archived over 100,000 aliquots. The Biorepository also manages the Dartmouth Biospecimen Storage Facility, a secure, off-site facility dedicated to long term storage of specimens, providing 24 hour/7 day a week, real-time video surveillance, continuous and redundant monitoring of freezer temperatures and emergency response to users.
COBRE Biorepository Services
Reagent/Collection Device Preparation Services
Acid washing Collection Containers
Pre-cleaning wipes
Preparation of RNAlater tubes
Specimen Receipt and Management Services
Pre-processing storage
Specimen Check-in Annotation
Specimen Processing/Archiving/Referral Data Management and Auditing
Initial Specimen Processing Services
Blood fractionation (adult, child, infant, cord)
Lymphocyte isolation from blood (adult, cord)
Urine aliquot processing (adult, child, infant)
Urinary Specific Gravity
Urinary Creatinine
Stool Processing (meconium, transitional stool, infant, child, adult)
Placenta and tissue processing (freezing, fixation)
Nail and hair processing
Guthrie Card Processing
Water Aliquot processing
Secondary/Pre-analytical Specimen Processing Services
DNA extraction, quantification and normalization: Genomic (Blood, Buffy Coat, Tissue, FFPE, Saliva, Swabs)
DNA extraction, quantification and normalization: Microbiome (Stool, Skin, Vaginal, Breastmilk, Saliva)
RNA extraction, quantification and normalization: Genomic (Blood, FFPE)
Reformat aliquoting between various storage format (tubes and plates)
Targeted/Map plating
Dilution plating
Post-Processing Services
Specimen Archiving (RT, 4°C, -20°C, -80°C, -150°, LN2)
Specimen Retrieval and Referral
Quality Control
Reference and Quality Control Material Management
Reference and Quality Control Maternal Creation and Characterization
Inclusion in Reference and Quality QC Materials Referrals
Quality Control Reference Material Results Review
Pilot/Study Consultation and Development
Protocol development and Validation
Services are offerred outside of Dartmouth Medical School
Consulting is offerred outside of Dartmouth Medical School
Air Monitor System Corp Chemical Fume Hood
Labco Logic(+) Purifier Class A2 Biosafety Cabinet
Velos eSample Specimen Management Software
Hamilton MicroLab Star Automated Liquid Handler
Hamilton Decapper-Capper
BMG Labtech SpectrostarNano Spectrophotometer
Zebra Stripe S4M Barcode Label Printer (2)
Zebra GX430T Barcode Label Printer
BioMicrolab 1D/2D Barcode Tube/Rack Reader
Motorola DS4208 1D/2D Barcode Scanners (8)
Eppendorf 5414 Centrifuge
Eppendorf 5418 Centrifuge (2)
Eppendorf 5418R Centrifuge
Eppendorf 5804 Centrifuge
Sorvall Legend RT Centrifuge
Compound Light Microscope
Hemacytometer
Lab Heating Blocks (2)
Vortex Genie 2 (3)
Vortex Genie Pulse Vortex
Disruptor Genie Homogenizor
Multi-Microplate Genie Plate Shaker
Precision Shaking Incubating Waterbath
Ohaus Microbalance
Symphony pH Meter
Atago PAL-10S Pocket Refractormeter
Rainin Classic Single Channel Pipette Lite 0.5-2ul
Rainin Classic Single Channel Pipette Lite 100-1000ul
Rainin Classic Single Channel Pipette Lite 2-200ul
Rainin Classic Single Channel Pipette Lite 2-20ul
Rainin E4XLS Electronic Multipipette 20-300ul
Rainin E4XLS Electronic Multipipette 2-20ul
Rainin Pipette Lite XLTS Multipipette 1000-1200ul
Rainin Pipette Lite XLTS Multipipette 20-300ul
Rainin Pipette Lite XLTS Multipipette 2-20ul
Pipette Aid
Color scanner/printer
Thermoscientific Locator 4 Plus Dewars (8)
Chest -150° C Freezers (2)
Hamilton Sample Access Module (SAM) -80° C Freezers (2)
Upright -80° C Freezers (18)
Upright -20° C Freezers (1)
Under Bench -20° C Freezer (2)
Walk-in 4° C Refrigerator
Refrigerator 4° C (6)
REES Scientific Centron Environmental Monitoring System (2)
Honeywell Environmental Monitoring System
Lenel Security System (Controled Access, Intrusion Detection, Video Surveilliance)
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