Agilent Seahorse XFe96 Analyzer

Seahorse XFe96 Analyzer measures OCR and ECAR of live cells in 96 well plate format. [Product Link]

RRID:SCR_019545

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BD FACSAria IIu High Speed Cell Sorter

Aria has four lasers, 405, 488, 561 and 633 nm, and can detect light in sixteen photomultiplier tubes. Its primary function is to examine complex populations of cells and yield pure populations of cells. The Aria can sort lymphocytes at a rate of approximately 15,000 events per second. For larger cells, the rate is much slower in order to optimize the passage of cells through the instrument. Cells from single populations can be sorted into 96-well plates. Alternatively, the Aria can sort into two 15-ml tubes or four 5-ml tubes. The Aria can purify samples that are simply positive and negative for a single fluorophore or as complex as nine-color samples with intricate gating schemes. The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 405 nm, 488 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 ?m, 85 ?m and 100 ?m nozzles. If you need a yellow-green (561 nm) laser, check the BD FACSAria III instead. [Product Link]

RRID:SCR_019598

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Beckman Coulter CytoFLEX Flow Cytometer

Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]

RRID:SCR_019627

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Beckman Coulter Gallios Flow Cytometer

Flow Cytometer provides efficient acquisition of up to 12 parameters to achieve accurate size measurement and high resolution and processing. [Product Link]

RRID:SCR_019639

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Bio-Plex® 200 Suspension Array System

The Bio-Plex® 200 system is a suspension array reader that provides a reliable multiplex assay solution permiting analysis of up to 100 biomolecules in a single sample. [Product Link]

RRID:SCR_018026

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Bio-Rad ZE5/Yeti Cell Analyzer Flow Cytometer

Cytometer with configurations to meet broad range of experimental complexities and throughput needs. Accessible for novice flow cytometry users yet flexible enough for most experienced flow cytometry professionals. [Product Link]

RRID:SCR_019717

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Cytek Aurora Spectral Analyzer

Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]

RRID:SCR_019826

Citation IDs: RRID:SCR_017788

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Miltenyi Bioscience MACSQuant Analyzer 10

MACSQuant Analyzer 10 brings to life the versatility and power required for modern flow cytometry applications. Whether mining for rare cells, analyzing the efficiency of your cell manufacturing process or investigating signaling pathways, you are equipped for the task at hand. With a range of automated features, this flow cytometer lays the foundation for true automation present in all instruments belonging to the MACSQuant Family of flow cytometers. [Product Link]

RRID:SCR_020268

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Sony Biotechnology SH800S Cell Sorter

The benchtop SH800S cell sorter is a cell sorter that sorts a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective and easier to perform. System software supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. The SH800S is for non-clinical research use only and is a Class 1 laser product. The SH800S is flexible and can support a variety of applications for individual and core labs. For applications in which cross-contamination is a concern, optional e-beam sterilized consumables can be used to replace the sample line and sorting chip. An optional class A2 Level II biosafety cabinet provides protection for personnel and products. The biosafety cabinet was custom designed for the SH800S and tested by The Baker Company to meet international standards. The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection. [Product Link]

RRID:SCR_018066

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