The SDSU Flow Cytometry and Live Imaging Core is a centralized, state-of-the-art shared resource facility housed in five dedicated laboratory rooms (878 sq. feet) within the North Life Sciences building. The Core is overseen by a Faculty Director and a Technical Director. All services are provided on a fee-for-service, hourly recharge basis and are available for BSL-1 and BSL-2 research activities. Major instrumentation includes a BD Biosciences FACSAria™ Fusion in a biosafety cabinet and FACSMelody™ cell sorting systems; two BD® LSR II flow cytometric analyzers; an Amnis® ImageStreamX Mark II imaging flow cytometer; a PerkinElmer In Vivo Imaging System (IVIS); and a MESO QuickPlex® Q 60MM electrochemiluminescence detection platform. The Core provides comprehensive instrumentation, expert technical support, and consultation in experimental design and data analysis that supports high-parameter cellular phenotyping (up to 18 colors), high-speed, multi-population sorting (up to four populations simultaneously) for enrichment or high-purity recovery, 96-well plate single-cell sorting for clonal expansion or downstream single-cell sequencing applications, multi-parameter quantitative imaging (up to six imaging channels), whole-organism fluorescence and bioluminescence imaging, and multiplex biomarker quantification.

The UCSD Cellular and Molecular Medicine Electron Microscopy core provides services in sample preparation and imaging by Transmission Electron Microscopy and Correlative Light and Electron Microscopy.

Core that provides and produces transgenic mice. Services and methodologies include pronuclear microinjection and blastocyst microinjection for creation of new mouse cell lines as well as archival services like embryo and sperm freezing.

Core which provides Diabetes Research Center investigators access to high-throughput sequencing, data acquisition and analysis. Its main services include Sample QC and Processing, Illumina Platform Library Preparation and Sequencing, and Expression microarrays (RNA, microRNA).

Our Facility houses a 600 MHz Bruker Avance III NMR spectrometer fitted with a 1.7mm triple resonance cryoprobe, for analysis of mass limited samples, and a 24 position sample changer for automated data acquisition. Users may collect their own data after suitable training, or have Facility staff do it for them.

The IGM Genomics Center at the University of California, San Diego, is a core research facility overseen by the IGM. Located in Leichtag 172, the IGM Genomics Center provides a variety of services, including sequencing library preparation and sequencing on Illumina\'s MiSeq and NovaSeq 6000 platforms. We support single cell sequencing on the 10X Genomics Chromium Controller. We also process Illumina Infinium Beadchips, which includes a variety of whole-genome genotyping (WGGT) arrays as well as the Infinium MethylationEPIC BeadChip.

Core facility that provides services to support stem cell research for Salk Institute researchers and their direct collaborators. The CTEC provides well-characterized human embryonic stem (hES) cell lines and human induced pluripotent stem (iPS) cell lines, space and equipment for research, as well as training and consultation.

Core offers single-cell and spatial omics services. Provides consultation, expert guidance in experimental design, and technologies tailored for innovative research in genomics. Facility is equipped with 10x Genomics Chromium X and Xenium systems, Vizgen MERSCOPE, Bruker/NanoString GeoMx Digital Spatial Profiler, and nCounter MAX.

Core facility established to assist the Salk community with integrating genomics data into their research. The primary focus of the core is to provide analysis support for next-generation sequencing applications.

Core facility that provides consultation on the use of viral vector technologies as well as custom design and production services for multiple vector types. The GT3 facilitates the use of these research tools by Salk researchers and others across diverse fields of study such as systems neuroscience, stem cell biology, metabolism, ageing, cancer biology and gene therapy. The GT3 core is a designated Cancer Center Council (C3) core facility. Cancer Center members from participating C3 institutes have preferential rates.

We provide a broad range of in vivo research services, primarily in mice but also rats, appropriate to neuroscience and other fields of biological research. We perform behavioral testing, compound dosing, tissue sampling, stereotaxic surgery and other surgeries on request and tissue recovery.

Core facilty that provides access and services for analyzing targeted and complex mixtures of protein and small molecules, like metabolites. By utilizing these tools, research on disease states can be augmented by proteomic and metabolomic study.

Core facility that provides media, plates, and commonly used buffers for molecular biology applications and culturing mammalian cells, C. elegans, yeast, and bacteria. The Media Preparation Core Facility is equipped with 3 automatic plate pourers supported by 2 programmable media kettles capable of producing up to 9 liters of agar or nutrient media per run under gentle and reproducible conditions. Plates are available in 6 cm or 10 cm formats.

Core facility that provides the resources to investigate sensory, motor and complex behavioral testing in rat and mouse models. The facility offers standard neurobehavioral tests, customized test batteries, training for independent use of the equipment, data interpretation and anlaysis.

Core facility equipped with commercial imaging and data analysis technologies. The facility will provide technical assistance to the Salk Institute faculty through access to onfocal microscopy (both fixed and live cell), TIRF microscopy, two-photon microscopy, electron microscopy and super-resolution microscopy as well as in vivo imaging modalities.

Core facility dedicated to providing high-throughput sequencing services, consultation and expert assistance in strategic planning and method development. The core houses state-of-the art equipment, including Illumina HiSeq 2500 and NextSeq 500 high-throughput sequencing systems, Agilent 2200 TapeStation, Covaris M220 Focused-ultrasonicator and a Bio-Rad QX200 Droplet Digital PCR system.

Core facility dedicated to advancing research projects requiring cell sorting and/or analysis of cell populations by flow cytometry. The core offers training on and access to shared use instrumentation for analytical flow cytometry, services for fluorescence activated cell sorting (FACS) as well as technical consultation and support on experimental design, execution, and data analysis.

The facility provides access to high-speed cell sorting, analytical flow and imaging flow cytometry, and validated antibody panels for immune profiling on the SBP campus in La Jolla. Trained SBP investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use. Trained external users have access during business hours.

Core staff provide technical expertise, hardware and software training, operate the facility‘s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.

Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.

Core provides Cryo-EM services focused mostly on Single Particle Analysis. Housed microscopes include a Titan Krios with Gatan K3 detector.

We support internal and external clients with Cryo-EM services focused mostly on Single Particle Analysis. Our microscopes include a Titan Krios with Gatan K3 detector.

The SBP Viral Vector Core Facility provides state-of-the-art viral vector-based gene/shRNA delivery technology. Its portfolio ranges from lentivirus, retrovirus, adenovirus, adeno-associated virus, zika virus, coronavirus, sindbis virus and vesicular stomatitis virus products as well as customized "The Works" viral vector construction and swapping service packages. There are over 50 ready-to-transduce reporter viruses available for live cell imaging and 2D/3D cell-based assays. For custom virus production, we can achieve high-titer, top quality viral particles suitable for in vitro and in vivo studies. The automated mini-scale preparations of vector plasmids and viruses in 96-well format can immediately allow downstream viral array screening. The core also extends technical expertise in non-viral nanoparticles which includes large extracellular vesicles and exosome purification, analysis and engineering.

The SBP Genomics Core provides end-to-end genomics solutions, from experimental design through sequencing, for academic and industry researchers. Our team offers full-service library preparation, high-accuracy sequencing on the Element Biosciences AVITI24, and access to the latest single-cell, spatial and epigenetic technologies.

Our services include:
- Next generation sequencing: RNA-seq, whole genome, whole exome and targeted sequencing
- Epigenetic profiling: DNA methylation, ATAC-seq, CUT&RUN, CUT&Tag and TIP-ChIP
- Single-cell multiomics: 10x Genomics Chromium X and Illumina PIP-seq
- Spatial transcriptomics and epigenomics: Curio Trekker, Illumina StrataMap, 10x Visium HD and GeoMx DSP
- In situ cell screening: Element Biosciences Cytos multiomic CRISPR and drug screening
- Sample QC, STR cell line authentication and instrument training

The SBP Functional Genomics Core Facility provides the infrastructure for cell-based gain-of-function (ORF, CRISPRa and CRISPRon) and loss-of-functioning (siRN, miRNA, shRNA, CRSIPRko, CRISPRi and CRISPRoff), libraries screening services, starting with assay development and carrying all the way through verification of identified targets. The large scale screens is accomplished via ready-to-use genome-wide or pathway-specific library platforms with choice of arrayed or pooled libraries. The FG core facility is equipped to perform custom CRISPR gene activation, knock-down, knock-out and editing services. We also adopt CRISPR nucleotide base editing technology and offer custom built CRISPR domain libraries. This approach can reveal the specific domain of a target protein interacting with another molecule, such as protein, IncRNA or drug in a native cell cellular environment.

The Sanford Burnham Prebys Proteomics Shared Resource provides state-of-the-art mass spectrometry-based proteomics services including identification, quantification and characterization of proteins and their post-translational modifications. We provide proteomics services to Institute scientists as well as outside non-profit and for-profits organization on a fee-for-service basis.

Academic drug discovery facility with advanced

The Sanford Burnham Prebys Cancer Metabolism Core provides a unique service for the analysis of metabolism, offering both metabolite quantification and stable-isotope tracing methodology, centered around gas chromatography-mass spectrometry (GC-MS). We offer GC-MS analysis of polar metabolites (TCA cycle, glycolysis metabolites and amino acids), short chain fatty acids, fatty acids and cholesterol, sugars and sugar-phosphates. As well as cells, we can also analyze tissues, tumors, serum or culture medium as necessary. We also have available a YSI 2950 analyzer for measurement of glucose, lactate and glutamine in medium samples. We collaborate closely with Core users and can provide in-depth advice on the direction of metabolic experiments, as well as the interpretation of results.

Core facility dedicated to providing a static or dynamic (in vitro/in vivo) imaging services by offering histology and microscopy services and providing support and training to interested researchers. The facility offers a range of light microscopes, related imaging systems and image analysis resources for assisted or independent use (upon training). It also can provide advice on proper sample fixation, processing, embedding of tissues with attention to desired orientation, decalcification of bone tissue, cutting of paraffin blocks, cryosectioning, routine and special stains as well as training in histological techniques.

Flow cytometry core which offers a wide range of routine and custom flow cytometry, mass cytometry, cell sorting services, data analysis, strategic planning for novel assays, user training, and full scale research collaborations.