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All Facilities >> Sanford Burnham Prebys Medical Discovery Institute >> Flow Cytometry Shared Resource (Flow Cytometry)
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10901 N. Torrey Pines Rd
La Jolla, CA 92037
United Stateshttps://sbpdiscovery.org/research/shared-resources/flow-cytometry/cite this facility
Yoav Altman
Last Updated: 05/20/2025
The facility provides access to high-speed cell sorting, analytical flow and imaging flow cytometry on the SBP campus in La Jolla. Trained SBP investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use. Trained external users have access during business hours.
Core staff provide technical expertise, hardware and software training, operate the facility\'s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.
Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.
Services are offerred outside of Sanford Burnham Prebys Medical Discovery Institute
Consulting is offerred outside of Sanford Burnham Prebys Medical Discovery Institute
Data Analysis And Interpretation
High Dimensional Flow Cytometry Analysis
Shared Instrumentation Oversight & Maintenance
BD FACSAria IIu High Speed Cell Sorter
Aria has four lasers, 405, 488, 561 and 633 nm, and can detect light in sixteen photomultiplier tubes. Its primary function is to examine complex populations of cells and yield pure populations of cells. The Aria can sort lymphocytes at a rate of approximately 15,000 events per second. For larger cells, the rate is much slower in order to optimize the passage of cells through the instrument. Cells from single populations can be sorted into 96-well plates. Alternatively, the Aria can sort into two 15-ml tubes or four 5-ml tubes. The Aria can purify samples that are simply positive and negative for a single fluorophore or as complex as nine-color samples with intricate gating schemes. The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 405 nm, 488 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 ?m, 85 ?m and 100 ?m nozzles. If you need a yellow-green (561 nm) laser, check the BD FACSAria III instead. [Product Link]
cite this instrument
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
cite this instrument
- BD FACSAria 12-color high-speed cell sorter with 407 nm, 488 nm, and 633 nm laser lines
- BD FACSAriaIIu 14-color high-speed cell sorter with 405 nm, 488 nm, 561nm, and 640 nm lasers
- BD LSRFortessa 14-color FACS analyzer with HTS plate loader for 96 or 384-well plates and four solid state lasers (405 nm, 488 nm, 561 nm, 640 nm)
- Amnis ImageStream MkII Imaging Flow Cytometer with two cameras and 405 nm, 488 nm, and 647 nm lasers
- BD FACSCanto 6-color FACS analyzer with 488 nm and 633 nm lasers
- BD FACSCalibur 4-color analyzer with 488 nm and 635 nm lasers
- Muse Cell Analyzer
Publications associated with this facility (Click To View):
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https://coremarketplace.org/?FacilityID=446Institution
Institution ROR ID: https://ror.org/03m1g2s55Keywords:
USEDit, ABRF, cell sorting, analytical flow cytometry
Resource Type:
access service resource, core facility, service resource
Citation:
Sanford Burnham Prebys Medical Discovery Institute Flow Cytometry Core Facility (RRID:SCR_014854)
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Transgenics
Pamela L Mellon
https://diabetescenters.org/cores/ucsd-ucla-transgenic-and-knockout-mouse-core
RRID:SCR_015096
Core that provides and produces transgenic mice. Services and methodologies include pronuclear microinjection and blastocyst microinjection for creation of new mouse cell lines as well as archival services like embryo and sperm freezing.
This facility does not provide services outside its institution
This facility does not consult outside its institution
10/09/2024
Epigenomics
RRID:SCR_015097
Core which provides Diabetes Research Center investigators access to high-throughput sequencing, data acquisition and analysis. Its main services include Sample QC and Processing, Illumina Platform Library Preparation and Sequencing, and Expression microarrays (RNA, microRNA).
This facility does not provide services outside its institution
This facility does not consult outside its institution
10/09/2024
Gene Manipulation
RRID:SCR_015099
Core provides services such as assistance in the development and successful completion of well-designed genetic studies, establishment and storage of EBV-transformed lymphoblastoid cell lines (LCLs), as well as PAXgene tubes, and specialized (e.g., the exome chip) single nucleotide polymorphism (SNP) testing, candidate gene sequencing, whole exome sequencing (WES), whole genome sequencing (WGS), and large-scale gene methylation analysis.
This facility does not provide services outside its institution
This facility does not consult outside its institution
10/09/2024
NMR Medicinal Chemistry Support
Nuclear Magnetic Resonance (NMR)
Nuclear Magnetic Resonance
Brendan Duggan
Skaggs School of Pharmacy and Pharmaceutical Sciences
9255 Pharmacy Lane
La Jolla, CA 92093-0657 - United States of America
RRID:SCR_017919
Our Facility houses a 600 MHz Bruker Avance III NMR spectrometer fitted with a 1.7mm triple resonance cryoprobe, for analysis of mass limited samples, and a 24 position sample changer for automated data acquisition. Users may collect their own data after suitable training, or have Facility staff do it for them.
This facility provides services outside its institution
This facility provides consulting outside its institution
07/13/2023
Genomics / Genome Analysis and Technologies
Kristen Jepsen
Leichtag Room 172
9500 Gilman Drive
La Jolla, CA 92093-0761 - United States
RRID:SCR_022740
The IGM Genomics Center at the University of California, San Diego, is a core research facility overseen by the IGM. Located in Leichtag 172, the IGM Genomics Center provides a variety of services, including sequencing library preparation and sequencing on Illumina\'s MiSeq and NovaSeq 6000 platforms. We support single cell sequencing on the 10X Genomics Chromium Controller. We also process Illumina Infinium Beadchips, which includes a variety of whole-genome genotyping (WGGT) arrays as well as the Infinium MethylationEPIC BeadChip.
This facility provides services outside its institution
This facility provides consulting outside its institution
09/13/2022
Consultations
Correlative Light Electron Microscopy
Electron Microscopy
Image Aquisition
Immuno Electron Microscopy
Microscopy
Microtomy/Electron
Sample Preparation
Transmission Electron Microscopy
Microscopy (Electron, Fluorescence, Optical)
Guillaume Castillon
Cellular & Molecular Medicine, GPL room 218A, 9500 Gilman Drive
La Jolla, CA 92093 - United States of America
RRID:SCR_022039
Other CIDs:S10OD023527
The UCSD Cellular and Molecular Medicine Electron Microscopy core provides services in sample preparation and imaging by Transmission Electron Microscopy and Correlative Light and Electron Microscopy.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/24/2022
Computational - Bioinformatics
Computational - Biostatistics
Computational - High Performance Computing
Data Analysis
Laser Capture Microdissection
Mass Spectrometry
Multiphoton Microscopy
oligo synthesis
Peptide Synthesis
Proteomics
Real-time qPCR
RNA analysis
Mass Spectrometry
Anne E. Stanley
Penn State College of Medicine, Section of Research Resources H093
500 University Drive
Hershey, PA 17033-0850 - United States of America
http://research.med.psu.edu/core-facilities/mass-spectrometry
RRID:SCR_017831
The Penn State College of Medicine Mass Spec and Proteomics Facility offers complete sample prep services, methods development, and bioinformatic/biostatistical analyses for discovery and targeted proteomics and quantitative proteomics (Identification & Quantitation w DIA-PASEF , plus targeted MRM/SRM assays) as well as for small molecule/lipid quantitation.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/12/2025
Cell Sorting
Data Analysis
Flow Cytometric Analysis
Laser Capture Microdissection
Bivian Torres
130 Scripps way, C276
Jupiter, FL 33458 - United States of America
https://www.scripps.edu/science-and-medicine/cores-and-services/flow-cytometry-ca/index.html
RRID:SCR_017830
The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps on a fee-for-service basis. Services offered include, cell sorting, analytic cytometry, laser microdissection and hematology.
FCC facility staff will perform all sorting on the FACS ARIA3.
Analytical applications include immuno-phenotyping, cell cycle analysis (DNA content, BrdU), and calcium flux (ratiometric imaging), among others. Additional modalities (FRET, intracellular organelle staining, etc.) can be developed in response to need; please talk to us about your specific application.
The Leica LMD laser capture microdissection microscope has 4X, 6.3X, 10X, 20X, 40X, and 63x objectives, automated motorized scanning and positioning, and remote stylus operated input.
The Hemavet 950 FS is an animal blood cell counter and differential analyzer. The Hemavet allows for a fast and complete five-part white blood cell differential, red blood cell analysis, and platelet analysis.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/11/2024
RRID:SCR_025728
Biorepository collection is comprised of blood samples, tissues and other biological materials donated by patients, coupled with information about their health, environment, lifestyle, medical care and responses to medical treatment. Biorepository is accredited by College of American Pathologists.
This facility does not provide services outside its institution
This facility does not consult outside its institution
09/17/2024
Immunology
Zissis Chroneos
United States of America
https://research.med.psu.edu/core-facilities/pulmonary-immunology-physiology/
RRID:SCR_025624
Core provides development and testing of small animal models to be used for pre-clinical pulmonary research. BSL2 facility that allows the assessment of pulmonary function related to airway hyper-responsiveness, ventilation-induced lung injury, postnatal lung development, airway remodeling, chronic inflammation, allergy, environmental exposures and infection. Services offered include oxygen profiling, infection and treatment; ability to deliver, measure and test pre-clinical potential of therapeutic interventions via aerosol; and housing of small animals.
This facility does not provide services outside its institution
This facility does not consult outside its institution
08/19/2024
10x Genomics
Antibody Sequencing
BioBanking
Cell Line Authentication
Computational - Bioinformatics
Computational - Biostatistics
Computational - High Performance Computing
Consultations
Copy Number Variation (CNV)
Customized Protocols
Data Analysis
Data Processing And Interpretation
Digital PCR
DNA Analysis
DNA Isolation
Droplet Digital PCR
Genome/Transcriptome Assembly And Annotation
Genomics
Genotyping
Library Services
Metagenomics Analysis
Methylation Analysis
Microarray
Microbiome
Molecular Library Services
Nanopore Sequencing
Nucleic Acid Extraction
OpenArray
Pathway Interpretation
PCR Arrays
Pharmacogenomic
Plate Reader
Quantitative DNA Sequence Mapping
Real-time qPCR
RNA analysis
RNA Integrity
RNA Isolation
RNA Modification Analysis
RNAseq
Sample Preparation
Seq-Well
Sequencing - Capillary Sequencing
Sequencing - DNA Sequencing
Sequencing - Next Generation Sequencing (NGS)
Single-Cell Analysis
Single-cell Sequencing
TaqMan Arrays
Tissue Extraction
Whole Exome Sequencing
Whole Genome Amplification (WG
Genomics / Genome Analysis and Technologies
Sirisha Pochareddy
500 University Dr. C2705
Hershey, PA 17033-2390 - United States of America
https://research.med.psu.edu/core-facilities/genome-sciences/
RRID:SCR_021123
Provides efficient and comprehensive genomic analyses.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/02/2024
Image Aquisition
Dr Jennifer Sloppy
500 University Drive
Hershey, 17033 - United States of America
https://research.med.psu.edu/core-facilities/atomic-force-microscopy-core/
RRID:SCR_025075
The Atomic Force Microscopy Core at Penn State College of Medicine was created in 2023, in part, through an NIH S10 award (1S10OD030279-01A1). The core’s mission is to provide the College of Medicine research community with access to state-of-the-art atomic force microscopy equipment and expertise as a means to advance the strategic research goals of the institution.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/26/2024
Analysis Workstations
EEG
Eye Tracking
Functional Brain Imaging
Magnetic Resonance Imaging (MRI)
Nuclear Magnetic Resonance (NMR)
Magnetic Resonance
Jeff Vesek
500 University Drive
30 Long Lane
Hershey, PA 17033 - United States of America
RRID:SCR_021198
MRI Laboratory
The MRI Core Facility contains 8,900 sq. ft. of laboratory space, which includes: biochemical, electronic and surgical suites, and a fully equipped machine shop with (2) 3D printers.
Equipment
The MRI Core facility has two MRI systems and an EEG MRI-compatible system dedicated for research.
3T Siemens PRISMA-Fit scanner equipped with True 2 Channel Tx and 64 Channel Rx. The latest RF coil set including a 64 Channel head coil and software packages with full clinical capability.
7T Biospec 70/20 as small animal imaging system (Bruker Biospin, Ettlingen, Germany). Latest Hardware and Software. Avance NEO with Paravision 360.
Full sensory fMRI stimulation systems:
SenSaVue System for visual and auditory stimulation (Invivo Corp, FL, USA), which includes an LCD visual display, audio system, button response unit and software (E-Prime) for paradigm creation, patient management, protocol planning, precise delivery of brain stimulation, and behavioral analysis.
ETT Olfactometer with full-programmable 6 independent channels for odor and trigeminal stimulation (ETT, LLC, Hershey, PA).
Olfact Smell Test System (Osmic Enterprises, Inc., Cincinnati, OH) is a computerized instrument to test the smell functions of human subjects. The smell functions that can be tested are odor threshold, odor identification, and odor memory.
ETT Gustatometer with full-programmable 7 independent channels for taste stimulation (ETT,LLC, Hershey, PA)
Eye Link 1000 Plus system via SR Research: The EyeLink 1000 Plus Host PC performs real-time eye tracking at 250, 500,1000, or 2000 samples per second while computing true gaze position on the display viewed by the participant. The Host PC also performs on-line detection and analysis of eye-motion events such as saccades, blinks, and fixations. In addition to the sample data, these events are stored in a data file on the Host PC. They can be sent through the Ethernet link to the Display PC with a minimal delay or output as analog signals (if the optional analog/digital I/O card is installed). From the Host PC, the operator performs participant setup, monitors their performance, and can communicate with applications running on a Display PC.
This facility provides services outside its institution
This facility provides consulting outside its institution
07/13/2023
Electron Microscopy
Microscopy
Sample Preparation
Shared Instrumentation Oversight & Maintenance
Structural Biology
Transmission Electron Microscopy
Microscopy (Electron, Fluorescence, Optical)
Jennifer Sloppy
500 University Drive
Hershey, PA 17033 - United States of America
https://research.med.psu.edu/core-facilities/microscopy-imaging/
RRID:SCR_021178
Located in Hershey, Pennsylvania.
Zeiss LSM 980 Cryo-Confocal Microscope
300 kV Krios Cryo-TEM for SPA and tomography, Equipped with Volta phase plates, bioquantum energy filter and K3 direct electron detector
200 kV thermionic JEOL 2100 for cryo-imaging, cryo-tomography, and standard imaging of stained grids
This facility provides services outside its institution
This facility provides consulting outside its institution
05/09/2023
Cell Sorting
Flow Cytometric Analysis
High Dimensional Flow Cytometry Analysis
Imaging Flow Cytometry
Phenotyping
Flow Cytometry
Nate Sheaffer
500 University Drive
Room C3603
Hershey, PA 17033 - United States of America
https://research.med.psu.edu/core-facilities/flow-cytometry/
RRID:SCR_021134
Flow Cytometry analysis, Cell sorting, Flow Imaging analysis
This facility provides services outside its institution
This facility does not consult outside its institution
05/09/2023
Consultation
Data Analysis
Data Analysis And Interpretation
Data Processing
Data Processing And Interpretation
Metabolomics
NMR Medicinal Chemistry Support
NMR Quality Control For Biologic Drugs
Nuclear Magnetic Resonance (NMR)
Structural Biology
Structure Determination
Nuclear Magnetic Resonance
Fang Tian
Penn State College of Medicine
Hershey, 17033 - United States
https://research.med.psu.edu/core-facilities/biomolecular-nmr/
RRID:SCR_023244
Our mission is to facilitate investigators to use multiple modern NMR applications, including structure elucidation of small molecules, triple resonance experiments of biomolecules, fragment-based drug discovery, metabolomic and tissue analyses.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/03/2023
In-vivo Imaging
Magnetic Resonance Imaging (MRI)
Preclinical Modeling / Imaging
Dr Kothapalli
500 University Drive
Hershey, 17033 - United States
https://research.med.psu.edu/core-facilities/comparative-medicine-imaging/
RRID:SCR_023179
The Penn State Hershey College of Medicine Comparative Medicine Imaging Core provides equipment and expertise for bioluminescence and fluorescence imaging, in-vivo, full-body, 3D anatomical, functional and molecular imaging and MRI.
This facility provides services outside its institution
This facility provides consulting outside its institution
01/21/2023
Jason Hughes
500 University Drive
Hershey, PA 17033 - United States
RRID:SCR_022953
High-Performance computing cluster housing x86 compute cores, graphical processing unit (GPU) cores, and directly-attached high-speed storage.
This facility does not provide services outside its institution
This facility does not consult outside its institution
11/08/2022
Antibody Characterization
Antibody Development
Antibody Production
Neil Christensen
500 University Drive
Hershey, PA 17033 - United States
https://research.med.psu.edu/core-facilities/custom-antibody-core/
RRID:SCR_022799
The Custom Antibody Biomedical Core Facility at the COM provides end-to-end service for the production and purification of custom antibodies
This facility provides services outside its institution
This facility provides consulting outside its institution
09/28/2022
Confocal Microscope With FCS/FLIM Capabilities
Confocal Microscopy
Confocal/ Super Resolution Microscope
Microscopy
Multiphoton Microscopy
Super-resolution Microscopy
Microscopy (Electron, Fluorescence, Optical)
Han Chen
500 University Drive, P.O.Box 850
Room C1730
Hershey, PA 17033 - United States
https://research.med.psu.edu/core-facilities/light-microscopy/
RRID:SCR_022526
The Light Microscopy Imaging Core at Penn State College of Medicine provides consultation and training in ultra-high-resolution imaging of cells and tissues in fixed or live states. The core also provides expertise in quantitative image analysis and consultations on microscopy-related research projects.
This facility provides services outside its institution
This facility provides consulting outside its institution
06/27/2022
Body Composition
Phenotyping
Scot R. Kimball, Ph.D.
700 HMC Crescent Road
Hershey, PA 17033 - United States
RRID:SCR_022565
The Metabolic Phenotyping Core at the Penn State College of Medicine offers equipment for the comprehensive assessment of energy balance and body composition in experimental rodents (mice and rats). Services include training in the use of the equipment, consultation on experiment design, and help with data analysis.
This facility does not provide services outside its institution
This facility does not consult outside its institution
04/26/2022
Electron Microscopy
Immuno Electron Microscopy
Sample Preparation
Transmission Electron Microscopy
Microscopy (Electron, Fluorescence, Optical)
Han Chen
500 University Dr.
Room C1727
Hershey, PA 17033 - United States of America
https://research.med.psu.edu/core-facilities/transmission-electron-microscopy/
RRID:SCR_021200
Our mission is to provide high-quality transmission electron microscopy (TEM) services, education, and training for the Penn State Hershey campus, other academic institutions, and private companies at large.
The lab is equipped with high-resolution transmission electron microscopes (JEOL 1400) and full suite of ancillary sample preparation equipment to fit most TEM-related needs. The equipment can provide nanometer scale analysis for biological, chemical and materials science. The TEM facility offers multiple levels of service that correspond with the services and training each user desires:
Project consultation
Education and training for new users
Full-service transmission electron microscopy.
TEM services include sample preparation, fixation, embedding, ultra-microtomy, staining, TEM imaging, immuno-localization (i.e. immunogold EM), and negative staining.
This facility provides services outside its institution
This facility provides consulting outside its institution
03/28/2022
Bioshipping
Blood Processing
Clinical Research
Clinical Trial Support
Biorepository
Susan George
500 University Drive
MC H171, Room C6529
Hershey, PA 17033 - United States of America
RRID:SCR_021194
Process, store and ship clinical trial samples from IRB approved studies. Collect remnant samples for an outside company for instrument correlation, validation and specimen type verification with IRB approved protocol. CLIA Waver for urine pregnancy, urine dipstick results for participant study treatment.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/11/2021
Biological photography/Photomicrography
Genotyping
Phenotyping
Protocol Development/Clinical Trial Coordination
Animal Facilities (Zebrafish, Fly, Mouse, Vivarium)
Khai C. Ang
500 University Drive
Room CG746
Hershey, PA 17033 - United States of America
RRID:SCR_021199
Other CIDs:G20OD016619
TobaccoCURE
The Zebrafish Functional Genomics Core at the Penn State College of Medicine was established to provide the Penn State research community with a modern, centralized facility for housing, breeding and performing experiments with zebrafish, one of the fastest-growing model systems in biomedical research.
The mission of the core is to provide a physical and intellectual infrastructure for investigators whose research can benefit from use of the zebrafish as a model system.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/11/2021
Nuclear Magnetic Resonance (NMR)
Pharmacology (Analytical, Research)
Xiangming Kong, Ph.D.
130 Scripps way
Jupiter, FL 33458 - United States of America
RRID:SCR_017834
Nuclear magnetic resonance, known as NMR, uses the magnetic properties of certain nuclei to study molecular structure. A wide variety of information can be gathered using NMR for chemical studies (including structural and conformational determinations) as well as biological studies (including protein and nuclei acid structure and function).
This facility provides services outside its institution
This facility provides consulting outside its institution
11/09/2020
X-Ray Crystallography
X-ray Diffraction and Scattering
HaJeung Park, Ph.D.
130 Scripps way
Jupiter, FL 33458 - United States of America
http://www.scripps.edu/florida/technologies/xray/contacts.html
RRID:SCR_017835
The core facility offers and operates as a full service core by performing crystallization, X-ray diffraction data collection (both in-house and synchrotron sources) processing, phasing, crystallographic refinement, model building, and visualization. The structural data obtained by the core will provide scientists with a wealth of information including but not limited to biological functions, 3D-folding, ligand binding (small molecule or protein), or mutational effect of target macromolecules.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/02/2019
Animal Husbandry
Alicia Faruzzi Brantley, PhD
130 Scripps way
Jupiter, FL 33458 - United States of America
The Behavior Core at Scripps Florida provides an excellent resource for investigators wishing to include behavioral assays in their research programs. It is divided into separate suites to accommodate either mice or rats, and a wide range of behavioral paradigms is available. Behavior Core personnel can assist in all aspects of rodent behavioral testing including design, training, and execution of behavioral tasks as well as data analysis and interpretation.
This facility provides services outside its institution
This facility provides consulting outside its institution
08/22/2014
Assays and Measurements
Cell Culture
Library Services
Molecular Library Services
Sahba Tabrizifard, Ph.D.
130 Scripps Way, 1B2
Jupiter, FL 33458 - United States of America
RRID:SCR_017832
The Cell Based High-Throughput Screening (CBS) core provides investigators with access to genome-wide collections of cDNAs, microRNA-adapted short hairpin (shRNA) retroviral libraries, and siRNA libraries that can be used to interrogate cellular models of signal transduction pathways and phenotypes. The core has also assembled a variety of smaller focus sets form each larger library as wells as chemical libraries available for cell-based screens.
This facility provides services outside its institution
This facility provides consulting outside its institution
08/22/2014
Data Analysis
DNA Analysis
Genomics
Microarray
Real-time qPCR
RNA analysis
RNA Integrity
Sequencing - Next Generation Sequencing (NGS)
Gustavo Martinez, Ph.D.
120 Scripps Way, 1B2
Jupiter, FL 33458 - United States of America
http://www.scripps.edu/florida/technologies/genomics/index.html
RRID:SCR_017827
The Genomics Core provides next generation sequencing and microarray analysis. The core supports a wide array of experimental approaches and respective bioinformatics support for:
RNA-Seq (mRNA, miRNA and total RNA)
Chromatin-associated RNA-Seq
ChIP-Seq
Whole gene expression profiling
Splice variant quantification
Copy number variation
Quantification of libraries used in pooled functional screening approaches
This facility provides services outside its institution
This facility provides consulting outside its institution
08/06/2014
Animal Husbandry
Assays and Measurements
Biochemical Analysis
Cell Culture
Melissa Kazantzis, PhD
120 Scripps Way, 1B2
Jupiter, FL 33458 - United States of America
http://www.scripps.edu/florida/technologies/metaboliccore/index.html
RRID:SCR_017848
At the Center for Diabetes and Metabolic Diseases (Metabolic Core), we offer prompt access to validated in vitro and in vivo tests to help advance the metabolic characterization of genetic and pharmacological research models of metabolic diseases, cancer, circadian rhythms, aging, inflammation and more.
We have consolidated analytical and in-vivo laboratories with a number of applications ranging from feeding, sleep, temperature, blood pressure, body composition and metabolic monitoring in mice, to cellular metabolism and in-depth chemical analysis of hormones, nutrients, inflammatory mediators and more.
Additionally, we will assist our users with trainings, experimental design and data analysis as needed and promptly respond to their troubleshooting requirements during experimentation.
This facility provides services outside its institution
This facility provides consulting outside its institution
08/05/2014
RRID:SCR_026396
Core offers single-cell and spatial omics services. Provides consultation, expert guidance in experimental design, and technologies tailored for innovative research in genomics. Facility is equipped with 10x Genomics Chromium X and Xenium systems, Vizgen MERSCOPE, Bruker/NanoString GeoMx Digital Spatial Profiler, and nCounter MAX.
This facility does not provide services outside its institution
This facility does not consult outside its institution
02/07/2025
Bioinformatics
April Williams
10010 N Torrey Pines Rd
San Diego, CA 92037 - United States of America
http://www.salk.edu/science/core-facilities/integrative-genomics-and-bioinformatics-core/
RRID:SCR_014842
Core facility established to assist the Salk community with integrating genomics data into their research. The primary focus of the core is to provide analysis support for next-generation sequencing applications.
This facility does not provide services outside its institution
This facility does not consult outside its institution
05/01/2024
John Naughton
10010 N. Torrey Pines Rd.
La Jolla, CA 92037 - United States
http://www.salk.edu/science/core-facilities/gene-transfer-targeting-and-therapeutics-core/
RRID:SCR_014847
Core facility that provides consultation on the use of viral vector technologies as well as custom design and production services for multiple vector types. The GT3 facilitates the use of these research tools by Salk researchers and others across diverse fields of study such as systems neuroscience, stem cell biology, metabolism, ageing, cancer biology and gene therapy. The GT3 core is a designated Cancer Center Council (C3) core facility. Cancer Center members from participating C3 institutes have preferential rates.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/01/2024
Behavioral Assessment
Behavioral Phenotyping
Cognitive Assessment
Discovery Science
Home Cage Behavioral Analysis
Pharmacokinetics
Phenotyping
Pre-Pulse Inhibition
Rotarod
Startle Response
Surgical Services
Nick Andrews
10010 N Torrey Pines Rd
La Jolla, CA 92037 - United States
We provide a broad range of in vivo research services, primarily in mice but also rats, appropriate to neuroscience and other fields of biological research. We perform behavioral testing, compound dosing, tissue sampling, stereotaxic surgery and other surgeries on request and tissue recovery.
This facility provides services outside its institution
This facility provides consulting outside its institution
03/10/2023
Cell Culture
Consultations
CRISPR
CRISPR Rosa26 & CRISPR H11
CRISPR/Cas Project Design
Directed Cell Differentiation
ES Cell Electroporation
Genome Engineering
Genotyping
Reprogramming Somatic Cells
Stem Cell Procurement
Tissue Culture
Jenn Page
10010 N Torrey Pines Rd
La Jolla, CA United States of America
RRID:SCR_014850
Core facility that provides services to support stem cell research for Salk Institute researchers and their direct collaborators. The STEM Core provides well-characterized human embryonic stem (hES) cell lines and human induced pluripotent stem (iPS) cell lines, space and equipment for research, as well as training and consultation.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/03/2023
Jolene Diedrich
United States of America
http://www.salk.edu/science/core-facilities/mass-spectrometry-core/
RRID:SCR_014843
Core facilty that provides access and services for analyzing targeted and complex mixtures of protein and small molecules, like metabolites. By utilizing these tools, research on disease states can be augmented by proteomic and metabolomic study.
This facility does not provide services outside its institution
This facility does not consult outside its institution
12/02/2022
Rebecca Bassett
United States of America
http://www.salk.edu/science/core-facilities/media-preparation-core/
RRID:SCR_014844
Core facility that provides media, plates, and commonly used buffers for molecular biology applications and culturing mammalian cells, C. elegans, yeast, and bacteria. The Media Preparation Core Facility is equipped with 3 automatic plate pourers supported by 2 programmable media kettles capable of producing up to 9 liters of agar or nutrient media per run under gentle and reproducible conditions. Plates are available in 6 cm or 10 cm formats.
This facility does not provide services outside its institution
This facility does not consult outside its institution
12/02/2022
Nick Andrews
United States of America
http://www.salk.edu/science/core-facilities/behavior-testing-core/
RRID:SCR_014840
Core facility that provides the resources to investigate sensory, motor and complex behavioral testing in rat and mouse models. The facility offers standard neurobehavioral tests, customized test batteries, training for independent use of the equipment, data interpretation and anlaysis.
This facility does not provide services outside its institution
This facility does not consult outside its institution
12/02/2022
Uri Manor
United States of America
http://www.salk.edu/science/core-facilities/advanced-biophotonics/
RRID:SCR_014838
Core facility equipped with commercial imaging and data analysis technologies. The facility will provide technical assistance to the Salk Institute faculty through access to onfocal microscopy (both fixed and live cell), TIRF microscopy, two-photon microscopy, electron microscopy and super-resolution microscopy as well as in vivo imaging modalities.
This facility does not provide services outside its institution
This facility does not consult outside its institution
12/02/2022
Elsa Molina
United States of America
http://www.salk.edu/science/core-facilities/next-generation-sequencing/
RRID:SCR_014846
Core facility dedicated to providing high-throughput sequencing services, consultation and expert assistance in strategic planning and method development. The core houses state-of-the art equipment, including Illumina HiSeq 2500 and NextSeq 500 high-throughput sequencing systems, Agilent 2200 TapeStation, Covaris M220 Focused-ultrasonicator and a Bio-Rad QX200 Droplet Digital PCR system.
This facility does not provide services outside its institution
This facility does not consult outside its institution
12/02/2022
Carolyn O'Conner
United States of America
RRID:SCR_014839
Core facility dedicated to advancing research projects requiring cell sorting and/or analysis of cell populations by flow cytometry. The core offers training on and access to shared use instrumentation for analytical flow cytometry, services for fluorescence activated cell sorting (FACS) as well as technical consultation and support on experimental design, execution, and data analysis.
This facility does not provide services outside its institution
This facility does not consult outside its institution
12/02/2022
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