The CoreMarketplace: UCSD-CMM-Electron Microscopy Core

The UCSD Cellular and Molecular Medicine Electron Microscopy core provides services in sample preparation and imaging by Transmission Electron Microscopy and Correlative Light and Electron Microscopy.

Facility Policies

Services are offerred outside of University of California, San Diego

Consulting is offerred outside of University of California, San Diego

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Services offered:

Consultations

Correlative Light Electron Microscopy

Electron Microscopy

Image Aquisition

Immuno Electron Microscopy

Microscopy

Microtomy/Electron

Sample Preparation

Transmission Electron Microscopy

Facility Equipment

TECNAI SPIRIT BIOTWIN transmission Electron Microscope
JEOL JEM 1400 plus transmission Electron Microscope

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Publications

Publications associated with this facility (Click To View):

1.) Ghatbale P, Sah GP, Dunham S, Khong E, Blanc A, Monsibais A, Garcia A, Schooley RT, Cobián Güemes AG, Whiteson K, Pride DT (2024 Dec 19). In vitro resensitization of multidrug-resistant clinical isolates of Enterococcus faecium and E. faecalis through phage-antibiotic synergy Antimicrobial Agents and Chemotherapy, 69(2), e00740-24. . PMCID: 11823633.

2.) Lam B, Amer L, Thompson E, Clark AE, Garretson AF, Carlin AF, Chen C, Retout M, Jokerst JV (2024 Dec 11). Matrix-Insensitive Sensor Arrays via Peptide-Coated Nanoparticles: Rapid Saliva Screening for Pathogens in Oral and Respiratory Diseases. ACS applied materials & interfaces, 16(49), 67362-67372. doi: 10.1021/acsami.4c15662. PMID: 39588945.

3.) Affonso de Oliveira JF, Moreno-Gonzalez MA, Ma Y, Deng X, Schuphan J, Steinmetz NF (2024 Dec 2). Plant Virus Intratumoral Immunotherapy with CPMV and PVX Elicits Durable Antitumor Immunity in a Mouse Model of Diffuse Large B-Cell Lymphoma. Molecular pharmaceutics, 21(12), 6206-6219. . PMID: 39526560.

4.) Karan S, Affonso De Oliveira JF, Moreno-Gonzalez MA, Steinmetz NF (2024 Nov 18). A Self-Amplifying Human Papillomavirus 16 Vaccine Candidate Delivered by Tobacco Mosaic Virus-Like Particles. ACS applied bio materials, 7(11), 7675-7683. . PMID: 39512153.

5.) Sutley-Koury SN, Taitano-Johnson C, Kulinich AO, Farooq N, Wagner VA, Robles M, Hickmott PW, Santhakumar V, Mimche PN, Ethell IM (2024 Nov 6). EphB2 Signaling Is Implicated in Astrocyte-Mediated Parvalbumin Inhibitory Synapse Development. The Journal of neuroscience : the official journal of the Society for Neuroscience, 44(45), . . PMID: 39327008.

6.) Omole AO, Affonso de Oliveira JF, Sutorus L, Karan S, Zhao Z, Neun BW, Cedrone E, Clogston JD, Xu J, Sierk M, Chen Q, Meerzaman D, Dobrovolskaia MA, Steinmetz NF (2024 Oct 24). Cellular fate of a plant virus immunotherapy candidate. Communications biology, 7(1), 1382. . PMID: 39443610.

7.) Homer CM, Voorhies M, Walcott K, Ochoa E, Sil A (2024 Oct 14). Transcriptomic atlas of the morphologic development of the fungal pathogen Coccidioides reveals key phase-enriched transcripts. bioRxiv : the preprint server for biology, (), . . PMID: 39463982.

8.) Watanabe R, Zyla D, Parekh D, Hong C, Jones Y, Schendel SL, Wan W, Castillon G, Saphire EO (2024 Oct 3). Intracellular Ebola virus nucleocapsid assembly revealed by in situ cryo-electron tomography. Cell, 187(20), 5587-5603.e19. . PMID: 39293445.

9.) Guo Z, Zhu AT, Wei X, Jiang Y, Yu Y, Noh I, Gao W, Fang RH, Zhang L (2024 Aug). A genetically engineered neuronal membrane-based nanotoxoid elicits protective immunity against neurotoxins. Bioactive materials, 38(), 321-330. . PMID: 38764446.

10.) Zhou Z, Mukundan N, Zhang JA, Wu YT, Zhang Q, Wang D, Fang RH, Gao W, Zhang L (2024 Aug). Macrophage-Mimicking Cellular Nanoparticles Scavenge Proinflammatory Cytokines in Specimens of Patients with Inflammatory Disorders. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11(31), e2401423. . PMID: 38884169.

11.) Venkatraman K, Budin I (2024 Aug). Cardiolipin remodeling maintains the inner mitochondrial membrane in cells with saturated lipidomes. Journal of lipid research, 65(8), 100601. . PMID: 39038656.

12.) Simms A, Zhao Z, Cedrone E, Dobrovolskaia MA, Steinmetz NF (2024 Jul 7). Cowpea mosaic virus intratumoral immunotherapy maintains stability and efficacy after long‐term storage Bioengineering & Translational Medicine, 9(6), e10693. . PMCID: 11558193.

13.) Islam MR, Youngblood M, Kim HI, González-Gamboa I, Monroy-Borrego AG, Caparco AA, Lowry GV, Steinmetz NF, Giraldo JP (2024 Jul 3). DNA Delivery by Virus-Like Nanocarriers in Plant Cells. Nano letters, 24(26), 7833-7842. doi: 10.1021/acs.nanolett.3c04735. PMID: 38887996.

14.) Geroyska S, Mejia I, Chan AA, Navarrete M, Pandey V, Kharpatin S, Noguti J, Wang F, Srole D, Chou TF, Wohlschlegel J, Nemeth E, Damoiseaux R, Shackelford DB, Lee DJ, Díaz B (2024 Jul 1). N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma. Cancer research communications, 4(7), 1815-1833. . PMID: 38949950.

15.) Oles RE, Terrazas MC, Loomis LR, Neal MJ, Paulchakrabarti M, Zuffa S, Hsu CY, Vasquez Ayala A, Lee MH, Tribelhorn C, Belda-Ferre P, Bryant M, Zemlin J, Young J, Dulai P, Sandborn WJ, Sivagnanam M, Raffatellu M, Pride D, Dorrestein PC, Zengler K, Choudhury B, Knight R, Chu H (2024 Jun 19). Pathogenic Bacteroides fragilis strains can emerge from gut-resident commensals. bioRxiv : the preprint server for biology, (), . . PMID: 38948766.

16.) Li M, Castro Lingl S, Yang J (2024 May 29). Reduction of hemagglutination induced by a SARS-CoV-2 spike protein fragment using an amyloid-binding benzothiazole amphiphile Scientific Reports, 14(), 12317. . PMCID: 11137076.

17.) Zhou Z, Ma A, Moore TM, Wolf DM, Yang N, Tran P, Segawa M, Strumwasser AR, Ren W, Fu K, Wanagat J, van der Bliek AM, Crosbie-Watson R, Liesa M, Stiles L, Acin-Perez R, Mahata S, Shirihai O, Goodarzi MO, Handzlik M, Metallo CM, Walker DW, Hevener AL (2024 Apr 5). Drp1 controls complex II assembly and skeletal muscle metabolism by Sdhaf2 action on mitochondria. Science advances, 10(14), eadl0389. . PMID: 38569044.

18.) González-Gamboa I, Caparco AA, McCaskill J, Fuenlabrada-Velázquez P, Hays SS, Jin Z, Jokerst JV, Pokorski JK, Steinmetz NF (2024 Mar 26). Inter-coat protein loading of active ingredients into Tobacco mild green mosaic virus through partial dissociation and reassembly of the virion Scientific Reports, 14(), 7168. . PMCID: 10965923.

19.) Xia W, Veeragandham P, Cao Y, Xu Y, Rhyne TE, Qian J, Hung CW, Zhao P, Jones Y, Gao H, Liddle C, Yu RT, Downes M, Evans RM, Rydén M, Wabitsch M, Wang Z, Hakozaki H, Schöneberg J, Reilly SM, Huang J, Saltiel AR (2024 Jan 29). Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation Nature Metabolism, 6(2), 273-289. . PMCID: 10896723.

20.) Amer L, Retout M, Jokerst JV (2024). Activatable prodrug for controlled release of an antimicrobial peptide via the proteases overexpressed in Candida albicans and Porphyromonas gingivalis. Theranostics, 14(4), 1781-1793. . PMID: 38389835.

21.) Zhu YP, Speir M, Tan Z, Lee JC, Nowell CJ, Chen AA, Amatullah H, Salinger AJ, Huang CJ, Wu G, Peng W, Askari K, Griffis E, Ghassemian M, Santini J, Gerlic M, Kiosses WB, Catz SD, Hoffman HM, Greco KF, Weller E, Thompson PR, Wong LP, Sadreyev R, Jeffrey KL, Croker BA (2023 Dec 22). NET formation is a default epigenetic program controlled by PAD4 in apoptotic neutrophils. Science advances, 9(51), eadj1397. . PMID: 38117877.

22.) Goldberg G, Coelho L, Mo G, Adang LA, Patne M, Chen Z, Garcia-Bassets I, Mesci P, Muotri AR (2023 Dec 21). TREX1 is required for microglial cholesterol homeostasis and oligodendrocyte terminal differentiation in human neural assembloids. Molecular psychiatry, (), . doi: 10.1038/s41380-023-02348-w. PMID: 38129659.

23.) Parra-Rivas LA, Madhivanan K, Aulston BD, Wang L, Prakashchand DD, Boyer NP, Saia-Cereda VM, Branes-Guerrero K, Pizzo DP, Bagchi P, Sundar VS, Tang Y, Das U, Scott DA, Rangamani P, Ogawa Y, Subhojit Roy (2023 Dec 20). Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function. Neuron, 111(24), 4006-4023.e10. . PMID: 38128479.

24.) Cobián Güemes AG, Ghatbale P, Blanc AN, Morgan CJ, Garcia A, Leonard J, Huang L, Kovalick G, Proost M, Chiu M, Kuo P, Oh J, Karthikeyan S, Knight R, Pogliano J, Schooley RT, Pride DT (2023 Dec 14). Jumbo phages are active against extensively drug-resistant eyedrop-associated Pseudomonas aeruginosa infections. Antimicrobial agents and chemotherapy, 67(12), e0065423. . PMID: 37931230.

25.) Venkatraman K, Lee CT, Garcia GC, Mahapatra A, Milshteyn D, Perkins G, Kim KY, Pasolli HA, Phan S, Lippincott-Schwartz J, Ellisman MH, Rangamani P, Budin I (2023 Dec 11). Cristae formation is a mechanical buckling event controlled by the inner mitochondrial membrane lipidome. The EMBO journal, 42(24), e114054. . PMID: 37933600.

26.) Daboussi L, Costaguta G, Gullo M, Jasinski N, Pessino V, O’Leary B, Lettieri K, Driscoll S, Pfaff SL (2023 Oct 28). Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair Cell reports, 42(11), 113282. . PMCID: 11034927.

27.) Kiratitanaporn W, Guan J, Berry DB, Lao A, Chen S (2023 Oct 26). Multimodal Three-Dimensional Printing for Micro-Modulation of Scaffold Stiffness Through Machine Learning. Tissue engineering. Part A, (), . doi: 10.1089/ten.TEA.2023.0193. PMID: 37747804.

28.) Ling C, Jin Z, Yeung J, da Silva EB, Chang YC, He T, Yim W, O'Donoghue AJ, Jokerst JV (2023 Oct 17). Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions. Chemical communications (Cambridge, England), 59(83), 12459-12462. doi: 10.1039/d3cc04142e. PMID: 37782049.

29.) Kaneko K, Liang Y, Liu Q, Zhang S, Scheiter A, Song D, Feng GS (2023 Oct 17). Identification of CD133(+) intercellsomes in intercellular communication to offset intracellular signal deficit. eLife, 12(), . . PMID: 37846866.

30.) Yim W, Retout M, Chen AA, Ling C, Amer L, Jin Z, Chang YC, Chavez S, Barrios K, Lam B, Li Z, Zhou J, Shi L, Pascal TA, Jokerst JV (2023 Sep 13). Goldilocks Energy Minimum: Peptide-Based Reversible Aggregation and Biosensing. ACS applied materials & interfaces, 15(36), 42293-42303. . PMID: 37651748.

31.) Retout M, Amer L, Yim W, Creyer MN, Lam B, Trujillo DF, Potempa J, O'Donoghue AJ, Chen C, Jokerst JV (2023 Sep 12). A Protease-Responsive Polymer/Peptide Conjugate and Reversible Assembly of Silver Clusters for the Detection of Porphyromonas gingivalis Enzymatic Activity. ACS nano, 17(17), 17308-17319. . PMID: 37602819.

32.) Venkatraman K, Lee CT, Garcia GC, Mahapatra A, Milshteyn D, Perkins G, Kim KY, Pasolli HA, Phan S, Lippincott-Schwartz J, Ellisman MH, Rangamani P, Budin I (2023 Sep 2). Cristae formation is a mechanical buckling event controlled by the inner membrane lipidome. bioRxiv : the preprint server for biology, (), . . PMID: 36993370.

33.) Liang W, Sagar S, Ravindran R, Najor RH, Quiles JM, Chi L, Diao RY, Woodall BP, Leon LJ, Zumaya E, Duran J, Cauvi DM, De Maio A, Adler ED, Gustafsson ÅB (2023 Aug 18). Mitochondria are secreted in extracellular vesicles when lysosomal function is impaired Nature Communications, 14(), 5031. . PMCID: 10439183.

34.) Zhao Y, Caron C, Chan YY, Lee CK, Xu X, Zhang J, Masubuchi T, Wu C, Bui JD, Hui E (2023 Jun 13). cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8(+) T cell function and anti-tumor immunity. Immunity, 56(6), 1187-1203.e12. . PMID: 37160118.

35.) xia W, Veeragandham P, Cao Y, Xu Y, Rhyne T, Qian J, Hung CW, Zhao P, Jones Y, Gao H, Liddle C, Yu R, Downes M, Evans R, Ryden M, Wabitsch M, Reilly S, Huang J, Saltiel A (2023 Jun 2). Obesity-dependent increase in RalA activity disrupts mitochondrial dynamics in white adipocytes Research Square, (), rs.3.rs-2923510. . PMCID: 10312969.

36.) Zhao Y, Caron C, Chan YY, Lee CK, Xu X, Zhang J, Masubuchi T, Wu C, Bui JD, Hui E (2023 May 8). Cis-B7:CD28 Interactions at Invaginated Synaptic Membranes Activate CD28 and Promote CD8+ T Cell Function and Anti-Tumor Immunity Immunity, 56(6), 1187-1203.e12. . PMCID: 10330546.

37.) Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T (2023 Apr 11). Identification of a Novel Small Molecule That Enhances the Release of Extracellular Vesicles with Immunostimulatory Potency via Induction of Calcium Influx ACS Chemical Biology, 18(4), 982-993. . PMCID: 10127211.

38.) Liang W, Diao RY, Quiles JM, Najor RH, Chi L, Woodall BP, Leon LJ, Duran J, Cauvi DM, De Maio A, Adler ED, Gustafsson ÃSB (2023 Feb 13). The Small GTPase Rab7 Regulates Release of Mitochondria in Extracellular Vesicles in Response to Lysosomal Dysfunction. bioRxiv : the preprint server for biology, (), . . PMID: 36824711.

39.) Affonso de Oliveira JF, Zhao Z, Xiang Y, Shin MD, Villaseñor KE, Deng X, Shukla S, Chen S, Steinmetz NF (2023). COVID-19 vaccines based on viral nanoparticles displaying a conserved B-cell epitope show potent immunogenicity and a long-lasting antibody response. Frontiers in microbiology, 14(), 1117494. . PMID: 37152732.

40.) Vijayakumar S, Nasr SH, Davis JE, Wang E, Zuidema JM, Lu YS, Lo YH, Sicklick JK, Sailor MJ, Ray P (2022 Dec 8). Anti-KIT DNA Aptamer-conjugated Porous Silicon Nanoparticles for the Targeted Detection of Gastrointestinal Stromal Tumors Nanoscale, 14(47), 17700-17713. . PMCID: 9744628.

41.) Vijayakumar S, Nasr SH, Davis JE, Wang E, Zuidema JM, Lu YS, Lo YH, Sicklick JK, Sailor MJ, Ray P (2022 Dec 8). Anti-KIT DNA aptamer-conjugated porous silicon nanoparticles for the targeted detection of gastrointestinal stromal tumors. Nanoscale, 14(47), 17700-17713. . PMID: 36416809.

42.) Sit I, Quirk E, Hettiarachchi E, Grassian VH (2022 Nov 29). Differential Surface Interactions and Surface Templating of Nucleotides (dGMP, dCMP, dAMP, and dTMP) on Oxide Particle Surfaces Langmuir, 38(49), 15038-15049. . PMCID: 9753757.

43.) Park DJ, Duggan E, Ho K, Dorschner RA, Dobke M, Nolan JP, Eliceiri BP (2022 Nov 5). Serpin-loaded extracellular vesicles promote tissue repair in a mouse model of impaired wound healing Journal of Nanobiotechnology, 20(), 474. . PMCID: 9636779.

44.) Waggoner LE, Kang J, Zuidema JM, Vijayakumar S, Hurtado AA, Sailor MJ, Kwon EJ (2022 Sep 21). Porous Silicon Nanoparticles Targeted to the Extracellular Matrix for Therapeutic Protein Delivery in Traumatic Brain Injury. Bioconjugate chemistry, 33(9), 1685-1697. . PMID: 36017941.

45.) González-Gamboa I, Caparco AA, McCaskill JM, Steinmetz NF (2022 Sep 16). Bioconjugation Strategies for Tobacco Mild Green Mosaic Virus. Chembiochem : a European journal of chemical biology, 23(18), e202200323. . PMID: 35835718.

46.) Monroy-Borrego AG, Steinmetz NF (2022 Aug 30). Three methods for inoculation of viral vectors into plants Frontiers in Plant Science, 13(), 963756. . PMCID: 9468707.

47.) Mantri Y, Sit I, Zhou J, Grassian VH, Jokerst JV (2022 Apr 21). Photoacoustic Enhancement of Ferricyanide-Treated Silver Chalcogenide-Coated Gold Nanorods The journal of physical chemistry. C, Nanomaterials and interfaces, 126(17), 7605-7614. . PMCID: 9563653.

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Associations

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Metadata

Links

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https://coremarketplace.org/?FacilityID=1324
https://coremarketplace.org/RRID:SCR_022039

Institution

Institution ROR ID: https://ror.org/0168r3w48

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, ABRF, imaging service, sample preparation service, Transmission Electron Microscopy

Resource Type:

access service resource, core facility, service resource

Citation:

University of California at San Diego Electron Microscopy Core Facility (RRID:SCR_022039)

UCSD-CMM-Electron Microscopy Core (Microscopy (Electron, Fluorescence, Optical))

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Facility Details

Facility Policies

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Facility Equipment

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Publications

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Associations

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Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Facility Profile QR Code (links to this profile)

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