Search for keywords in CoreMarketplace profiles. To search for a phrase, enclose it in double quotes.
Search for CoreMarketplace profiles by institution
Searching
All Facilities >> Rensselaer Polytechnic Institute >> Microscopy and Analytical Imaging Research Resource Core Laboratory (MAI) (Microscopy (Electron, Fluorescence, Optical))
Facility Details
About This Facility
Services and Equipment
Publications
Awards & Associations
Metadata
Collaboration:
Find Regional Facilities
Other Facilities at this Institution
cite this facility
Eduardo Rosa-Molinar, Ph.D.
Last Updated: 05/19/2025
The Microscopy and Analytical Imaging Research Resource Core Laboratory (MAI; also known as
Microscopy and Analytical Imaging Shared Resource [MAISR]), is a 2,450 ft2 core research resource core laboratory in Room 1043 in Haworth Hall at the University of Kansas Lawrence campus. The MAI (https://mai.ku.edu/) is a fee-for-service center. The imaging resources within the MAI are available to all University of Kansas, University of Kansas Medical Center, and University of Kansas Cancer Center students, staff, and faculty, students, staff, and faculty within and outside the state, and industry collaborators working in the life sciences and material sciences for nominal fees.
The MAI resource scientists (https://mai.ku.edu/lab-staff) provides training for undergraduate, graduate students, postdoctoral researchers, faculty, staff, and industry collaborators as many times as is necessary to master a given instrument and/or technique.
The MAI is directed by Eduardo Rosa- Molinar, Ph.D., Professor of Pharmacology and Toxicology, Bicampus Neuroscience Graduate Program, Anatomy and Cell Biology, and Bioengineering.
Services are offerred outside of Rensselaer Polytechnic Institute
Consulting is offerred outside of Rensselaer Polytechnic Institute
Correlative Light Electron Microscopy
Data Processing And Interpretation
Processing & Embedding- Histology
Hitachi 8100 Transmission Electron Microscope
Hitachi H-8100 TEM is a research grade scanning/transmission electron microscope (STEM) with analytical and high resolution imaging capabilities. Systematic theoretical instruction combined with practical hands-on training is the best way to thoroughly understand the utility and capabilities of this complex and delicate instrument. These instructions are meant only as a guide to the basic, daily alignments and operations of the H-8100, i.e. general imaging and diffraction. It is by no means a comprehensive guide to all operations of the microscope including ultra-high resolution imaging, SEM, EDX, cryo, and the various diffraction techniques. These advanced, specialized techniques will be dealt with on an as-need basis after mastery of the basic operations has been demonstrated. [Product Link]
cite this instrument
Hitachi S4700 Field Emission Scanning Electron Microscope
Hitachi S-4700 is a field emission scanning electron microscope (FE-SEM). More than a standard SEM, the S-4700, under optimal conditions, can magnify images upwards of 500,000 times and resolve features to 2 nanometers. In addition, a cathodoluminescence detector, backscatter electron detector and energy dispersive x-ray spectrographic detector accompany the microscope. [Product Link]
cite this instrument
Leica EM FC6 UC6b Ultramicrotome
Leica Ultracut EM UC6 is an advanced ultramicrotome that features a Touch Screen Control Panel or a Keypad Control Panel. Both options enable fast and safer alignment of knife and specimen, and the programmable knife and cutting movements make trimming easy. With Leica's AutoTrim, you can automatically trim a specimen to a predetermined level in the block face, best for morphometric studies. Three dependent, built-in, brightness- controlled LED light sources provide outstanding illumination for top light, back light, and specimen transillumination. [Product Link]
cite this instrument
Leica TCS SP2 Confocal Laser Scanning Microscope
Leica TCS SP2 is a spectral confocal laser scanning microscope with 405 UV laser 3CHDIC digital emission 400-800nm range designed to acquire high spatial resolution images of fluorescently labeled materials and for analysis of these images. The confocal principle utilizes a pinhole (confocal aperture) to eliminate out-of-focus light from fluorescently labeled specimens (i.e., provide “optical sectioning�). Lasers provide intense, point illumination that is scanned over the preparation and the fluorescence at each point is quantified and used to construct a representation of the object brightness (an image). This method provides high resolution for the x and y planes as well as vertically (z plane). The SP2 is an advanced confocal system with nine laser excitation lines spanning the spectrum from UV to near IR. The following laser lines are available: 405nm, 458, 476, 488, 496, 514, 543, 594, and 633, all fiber coupled and controlled by AOTFs. [Product Link]
cite this instrument
Olympus IX 81 Inverted Fluorescence Automated Live cell Microscope
Olympus IX81 Inverted Fluorescense Phase Contrast Live cell Inverted microscope. [Product Link]
cite this instrument
Major equipment available:
The MAI houses five light microscopes. These include:
1. 3i/Olympus (inverted) epifluorescence optical microscope, Olympus IX 81 inverted microscope with ZDC, Prior H117 with Proscan III stage, Andor Zyla 4.2 CMOS camera, dual excitation sources, a
Lambda LS Xenon and a white LED coupled to a Sutter Lambda 10-3 filter wheel. Olympus.
Objectives
– 10X 0.3NA UplanFL N, 20X 0.5NA UPlanFL N, 20X 0.75NA, 40X 0.6NA LUCPlanFL N, 40X 0.95NA UPlanSApo, 60X 0.7NA LUCPlanFL N, UPlanApo 60X 0.9NA, 100X 1.4NA UPlanSApo and 150X 1.45NA UApo N. Phase imaging available with 10X 0.3NA UplanFL N and 40X 0.6NA LUCPlanFL N, DIC imaging available with 20X 0.75NA, 40X 0.95NA UPlanSApo, and UPlanApo
60X 0.9NA.
Images acquired with SlideBook 6.0 using a workstation equipped with an Intel i7-6800k
processor, 32GB RAM, NVIDIA Quadro K620 graphics card, two- 256GB SSD RAID, one-2TB SATA HDD, running Windows 10 64 bit.
2. 3i/Olympus (inverted) spinning disk confocal microscope, Olympus IX-81 inverted microscope, Prior H117 with Proscan II stage, Hamamatsu Flash 4.0 v1 CMOS camera, Sutter Lambda 10-3 filter wheel, Yokagawa CSU10 spinning disk.
Olympus Objectives – 10X 0.3NA UplanFL N, 20X 0.75NA, 40X0.95NA UPlanSApo, 40X 1.3NA UPlanFL N, 60X 0.7NA LUCPlanFL N, 60X 1.42NA PlanApo N,100X 1.4NA UPlanSApo, 150X 1.45NA UApo N, CUBE 405nm laser, Sapphire 488nm laser,CrystaLaser 561 nm laser, CUBE 640nm laser, as well as the option of using a 7-line laser diode illuminator. Images acquired with SlideBook 6.0 using a workstation equipped with an Intel Xeon W3520 processor, 12GB RAM, NVIDIA Quadro FX 3500 graphics card, two-512GB SATA HDD RAID, running Windows 7 64bit.
3. Leica TCS SPE Confocal Laser Scanning Microscope (cLSM), Leica DM6-Q upright microscope, programmable piezo driven automated XYZ stage, 12-bit spectral PMT detector.
Leica Objectives –
10X 0.3NA Apo, 40X 1.15NA Apo,
Olympus Objectives - 10X 0.3NA UplanFL N, 20X 0.75NA, 40X 0.95NA UPlanSApo, 40X 1.3NA UPlanFL N, 60X 0.7NA LUCPlanFL N, 60X 1.42NA PlanApo N,100X 1.4NA UPlanSApo, 150X 1.45NA UApo N,
equipped with 405nm laser, 488nm laser, 561nm
laser, 637nm laser Images acquired with Leica LAS X using a workstation equipped with Intel i7-6800k
processor, 32GB RAM, NVIDIA Quadro K620 graphics card, two-256GB SSD RAID, one-2TB SATA
HDD, running Windows 10 64 bit.
4. Leica DM750 compound bright field upright microscope with a EXLite™ LED illumination, color
white light, a ICC50 W camera (5-megapixels) capable of connect live-stream HD to smartphones and tablets (IOS and Android), and with integrated SD card slot. Leica Objectives – 4X 0.10NA HI Plan, 10X 0.25NA HI Plan, 40X 0.65NA HI Plan and 100X 1.25 HI PLAN/Oil. Images acquired with Leica LAS CORE Imaging Software in a workstation with Intel i7-2600k processor, Intel HD Graphics card, 16 GB RAM and Windows 10 64bit.
5. The MAI houses a multi-photon excitation (MPE) microscope designed and built by Dr. Rosa-Molinar and his research group through funding from the Department of Pharmacology and Toxicology and BiCampus Neuroscience Graduate Program in collaboration with Intelligent Imaging Innovations, Inc. (3i).
This MPE microscope has the ability not only to acquire images deeper through living cells and tissue, but also integrates multiple quantitative capabilities such as, fluorescence lifetime imaging (FLIM) and time-resolved fluorescence anisotropy (polarization), to better understand physicochemical dynamics of cells, such as protein-protein interaction, protein and ligand interaction, molecular pathway analysis, and
correlative microscopy.
The Zeiss Axio Examiner.Z1 is a fully motorized fixed stage upright two-photon excitation microscope workstation with a Vector™ scan head with dual galvo scanning, mBeam™ remote beam expansion, pockels cell modulator, beam delivery optics and shielding, fully integrated dual six-core computer workstation with 32GB RAM, SlideBookTM software, installation, and on-site training, four GaAsP PMTs with automatic overload and computer-controlled reset, complete filter set for multiphoton including dichroics and emission filters for simultaneous multi-color imaging (Examiner slider: 750 edge; Nosepiece input: 680 SP; Nosepiece cube: 445/45, 495 edge, 525/40; TFP/YFP: 520 LP edge, 494/34, 542/27; TFP/YFP Wide: 520 LP edge, 488/50, 550/49; Cactus input: 720 SP: Cactus cube, red/far red 617/73, 660 edge, 692/40), Additional filter cube for polarized light imaging (Cactus cube, polarized light: 525/40, s polarizer, 529/23 polarizing bandpass, p polarizer), Rapid 90° switching of the polarization of the excitation beam, Piezo objective focusing collar with a 400μm range for high-speed
volumetric imaging, Microscope filter cube for GFP for viewing epifluorescence by eye, Motorized
ZDeck system with ProScan XY stage, ProScan III controller, joystick, universal holder, solid aluminum plate and pillar spacers for Zeiss Axio Examiner.Z1, X-Cite 120LED mini white light LED system with direct coupler for Zeiss Axio Examiner.Z1, W Plan-Apochromat 20x/1.0NA water dipping objective for Zeiss Axio Examiner.Z1; working distance 2.4mm, extended IR transmission (at least 60% at 1300 nm),
48" x 60" x 4.5" aluminum optical (breadboard top) vibration-isolation table, Fully interlocked laser
safety enclosure. The two-photon excitation microscope workstation is equipped with the Chameleon Discovery, a versatile, hands-free, and fully integrated ultra-fast femtosecond laser system with a complete tuning range of 680-1300 nm. The Chameleon Discovery laser has a second output that is a fixed 1040 line, which has been combined with the tunable line to accommodate dual 2Pexcitation. One workstation equipped with an Intel Zeon E5-2620 processor, 64GB RAM, NVIDIA Quadro M2000 graphics card, one-512 SATA HDD, running Windows 10 64 bit.
MAI also houses two electron microscopes. These include:
1. Hitachi H-8100 lanthanum hexaboride (LaB6) Transmission Electron Microscope (TEM) is housed in room 1043E of the MAI. The Hitachi H8100 LaB6 TEM is equipped with an assortment of single and
double tilt, single-specimen, beryllium room temperature holders and can operate at 75 kV, 100 kV, 150kV, 175 kV, and 200 kV (75 to 200 kV), making it possible to observe biological and non-biological materials of a wide range of thickness. The Hitachi H8100 LaB6 TEM is also equipped with a Advanced Microscopy Techniques (AMT) BioSprint high speed 16 megapixel (MP) low-mount CCD camera which provides high resolution digital capture of publication-quality images. The AMT BioSprint 16 MP low-mount CCD camera is controlled by a DELL Optiplex 7070, 64 bit mini tower workstation with Intel Core i7-9700 (8 Cores/12MB/8T/up to 4.7GHz/65W), 32GB 2x16GB DDR4 2666MHz UDIMM Non-ECC, AMD Radeon R5 430, 2GB, FH, 1TB Solid State SD Hard Disk Drive, 3.5" 1TB 7200rpm SATA Hard Disk Drive, Samsung 28-Inch UE570 UHD monitor, DVD-ROM Drive, and 8x DVD+/-RW 9.5mm Optical Disk Drive, running Windows 10 Professional 64bit. The Hitachi H8100 LaB6 TEM uses hollow-cone beam illumination, a beam illumination method by which a cone shaped electron beam is produced to perform diffraction. In hollow-cone beam illumination, the beam is tilted to a certain angle against the optical axis by the first-stage deflection coils and is tilted back by the second-stage deflection coils to illuminate the same position on the specimen, then, the beam is rotated
with respect to the optical axis keeping the same illumination position. Hollow-cone beam illumination is used to eliminate diffraction contrast in bright- and dark-field images and to observe the symmetries produced only by higher-order Laue zone (HOLZ) intensity bands without strong intensity due to zeroth-order Laue zone (ZOLZ) intensity band interaction in the convergent beam electron diffraction
(CBED) pattern, composed of non-overlapping disks (Kossel-Möllenstedt [K-M] pattern) for small
convergence angles and over-lapping disks (Kossel [K] pattern) for large convergence angles.
2. Hitachi S-4700 Type II Cold Field Emission Scanning Electron Microscope (CFE-SEM) is housed in room 1043F of the MAI. The Hitachi S-4700 Type II CFE-SEM is equipped with a new generation cold
cathode field emission gun, computer-controlled flashing, extraction and accelerating voltage, Mild
flashing function, Anode heater, Accelerating voltage 500V to 30kV, Landing voltage 0.01 - 2 kV (with deceleration), Magnification 20x to 1,000,000x (referenced to 4”× 5” photo), SE resolution 0.8 nm (15 kV), 1.1 nm (1kV landing voltage), Optical Modes, Normal probe current, optimized for high resolution imaging, High probe current, increased beam current, without significant loss of resolution, for analytical techniques, Ultra high resolution, optimized for short working distances, High resolution,
optimized for long working distances, Triple Secondary Electron Detector (SED) configuration, with ExB in-column filtering, Lower SED for standard topographic imaging, Upper through-the-Lens (TTL) SED for high resolution imaging, Top TTL SED, for high resolution imaging with adjustable high pass filter function, In-lens collection system for imaging of low and/or high angle Backscattered Electron (BSE)signals, SE/BSE signal selection and mixing for TTL detectors, YAG Backscattered Electron (BSE) detector, Electromagnetic beam blanker, with automated blanking when image is frozen, 5-position objective lens aperture with built-in anticontamination heater, 6” Diameter Specimen Exchange airlock, with two touch holder exchange, 5-axis automated stage with selectable speed, Full coverage of up to a 150mm diameter sample, Specimen navigation via Rapid Image Shift Movement (RISM) and Image Navigator with optical image, Built-in specimen anti contamination device, Energy saving (ECO)
mode: 0.3kVA power consumption, One workstation equipped with an Intel Xeon E3-V3, 4GB RAM, NVIDIA Quadro K620 graphics card, one-1TB SATA HDD, running Windows 7 32 bit.
MAI houses in the KU School of Engineering's Learned Engineering Expansion Phase 2 (LEEP-2) building, room G442, houses:
1. PHI 5000 VersaProbeII XPS/AES (Physical Electronics Inc., USA) ultrahigh vacuum instrument is a raster scanned, micro focused, x-ray beam. A 20 kV electron gun, with a LaB6 emitter, is used to
create a raster scanned electron beam that is focused onto the Al anode of the x-ray source in order to create a monochromated x-rays. An ellipsoidal shaped quartz crystal monochromator collects, filters, and refocuses the x-rays onto the sample surface to scan and acquire the spectrum. VersaProbeII is equipped with dual beam charge neutralizer, sputter depth profiling (5V to 5000, Angle resolved spectroscopy, chemical state imaging, elemental composition and chemical mapping with SXI (Secondary X-ray Imaging) and Scanning Electron Microscopy (SEM; using secondary electrons), five axis automated sample manipulator with extra sample prep chamber capable for heating, cooling and reaction under mixture of different gases. The X-ray beam size for XPS ranges from 9µ to 200µ and AES is scanned at 100nm. The instrument is driven by SmartSoft software and analysis of thedata is acquired by Multipak Software tools developed by Ph ysical Electronics using a workstation equipped with a Xeon E5-1603, 4GB RAM, NVIDIA Quadro 410 graphics card, two-512GB SATA HDD RAID, running Windows 7 32 bit.
Other resources available:
The MAI contains two offline workstations with Wacom Cintiq 22 pen display. Each computer is equipped with an Intel i7-5930K processor, 128GB RAM, AMD Radeon R9 390 graphics card, one-512 SSD, one-6TB SATA HDD, running Windows 10 64 bit, one workstation equipped with an Intel i7-5930K processor, 128GB RAM, AMD Radeon R9 390 graphics card, one-512 SSD, one-6TB SATA HDD, running Windows 7 64 bit, HTC VIVE VR Headset, one workstation equipped with an AMD FX 6350 processor, 32GB RAM, AMD Radeon HD 7700 graphics card, one-128GB SSD, one-1TB
SATA HDD, running Windows 7 64 bit, one workstation equipped with an Intel Xeon 6-core
processor, 64GB RAM, ATI Radeon HD 5870 graphics card, one- 1.5TB SSD RAID, one-2TB
SATA, running MAC OS 10.9.5.
The MAI staff workstations include: two laptop workstations equipped with an Intel i7-6700HQ processor, 64GB RAM, NVIDIA Quadro M2000M, one-512GB SSD, running Windows 10 64 bit, one laptop workstation equipped with an Intel i7 4810MQ, 32GB RAM, NVIDIA Quadro K5100M graphics card, one-512GB SSD, one-1TB SATA HDD, running Windows 10 64 bit, one workstation equipped with Intel Xeon W3520 processor, 12GB RAM, AMD FirePro V3900 graphics card, one-512GB SATA HDD, running Windows 10 64 bit.
To support cell culture, cellular and tissue imaging activities we have at our disposal within the MAI
(Haworth Hall 1043) and within my laboratory (Haworth Hall 1036; 3015; 3015A) the following
assets:
1. Nikon Eclipse TE2000-E Inverted Microscope (n=1)
2. Nikon Eclipse TE2000-U Inverted Microscope (n=1)
3. Nikon SMZ 445 Stereo Zoom Microscopes (n=2)
4. Nikon Eclipse 80i Upright Microscope (n=1)
5. VWR Incubating Orbital Shaker (n=1)
6. VWR unstirred water bath to 60° C (n=2)
7. Three CO2 incubators: (1) Barnstead/Lab-Line, Model 314, (2) Shel-Lab, Sheldon Manufacturing,
Inc., Model SCO6AD, and (3) VWR CO2 incubator with 2 shaker shelves and three electric outlets
inside, multi-position stirrer and hypoxia chamber
8. Class II, Type 2 Purifier Biological Safety Cabinet, Labconco (n=2)
9. PELCO BioWave® Pro+ Microwave Processing System (n=1)
10. Leica EM UC6 ultramicrotome (n=1) with 35° 45° (n=1) diamond knives
11. Sorvall MT-2 (n=2) and MT-2B (n=5) ultramicrotomes with 35° (n=7), 45° (n=7), Gumbo Histo (n=1), and oscillating (n=1) diamond knives
12. Sorvall TC-2 Tissue Slicer (n=1)
13. Sorvall GKM Knifemaker (n=2)
14. Leica EM KMR3 Knifemaker (n=1)
15. American Optical Spencer 860 Sliding Block Microtome (microtome knife blade 185 mm 7.25 inch) with Cryo-Histomat MK-2 controller, 75mm x 80mm stage freezing stage, closed circuit cooling
system and (n=1)
16. American Optical Spencer 820 Rotary Microtome with AO 820 Blade Holder #814, High
Profile Disposable Blades, LAB-TEK AO820 cassette vise jaws with floatation bath workstation(n=1)
17. Quorum Technologies carbon/metal evaporator/plasma cleaning (n=1)
18. Quorum Technologies critical point dryer (n=1)
19. Cryopress Slam Freezer (n=1)
20. FEI Vitrobot (n=1)
21. Cold storage facilities consisting of a liquid nitrogen freezer (n=1); cryogenic storage Dewars (n=6);
ultra-low upright freezer to -85° C (n=1), laboratory freezers to -15° C (n=4) and laboratory
refrigerators to 4° C (n=4)
Publications associated with this facility (Click To View):
Links
All the links listed resolve to this core profile
https://coremarketplace.org/?FacilityID=1205Institution
Institution ROR ID: https://ror.org/01rtyzb94Keywords:
USEDit, ABRF, sample preparation, imaging technologies, imaging
Resource Type:
access service resource, service resource, core facility
Citation:
University of Kansas Microscopy and Analytical Imaging Research Resource Core Facility (RRID:SCR_021801)
Select a distance from facility (direct distance between two points)
Filter Search Results
Filter results by resources
uncheck all
Filter results by services
uncheck all
Filter results by equipment
uncheck all
Amino Acid Analysis
Assays and Measurements
Biochemical Analysis
Cell Sorting
Flow Cytometric Analysis
Gel Chromatography
Light Scattering
Protein Extraction/Purification
Shared Instrumentation Oversight & Maintenance
Spectroscopy
Surface Plasmon Resonance (SPR)
Leslie Eisele
150 New Scotland Avenue
Center for Medical Sciences
Albany, NY 12208 - United States of America
The Biochemistry and Immunology Core provides access to specialized methods, technical instrumentation and expertise. The core focuses on purification, analysis, characterization and physical measurements, many in solution (native conditions) and complimentary to one another. This ranges from amino acids – structural studies – interactions – assemblies – to live cells. The core staff help investigators design experiments, collect, analyze and interpret data.
We are a combined facility: Biochemistry includes instrumentation for protein purification, analysis and physical measurements. Purification and analysis include various forms of liquid chromatography (UPLC, HPLC). Physical measurements can be achieved by a variety of spectrophotometric techniques (circular dichroism, ultra-violet, visible, and fluorescence spectroscopy), dynamic light scattering, SECMALLS, SPR and analytical ultracentrifugation.
Immunology instrumentation includes flow cytometry; instrumentation used to assess the immunophenotype of cell types and specific cell receptor(s), ligand binding, expression of surface and intracellular antigens, signal transduction events such as calcium flux, cell cycle progression based on analysis of DNA and RNA, apoptosis and cell sorting (including single cells).
A selection of shared instrumentation is available to researchers at the Wadsworth Center as well as scientists from neighboring institutes. The core is provides easy access to highly technical instrumentation and highly specialized methods not other wise readily accessible.
Chromatography equipment (HPLCs, UPLC)
Analytical Ultracentrifuge
Fluorescence Spectroscopy
UV/Vis spectroscopy
Circular Dichroism Spectroscopy
Dynamic Light Scattering
SECMALLS
Surface Plasmon Resonance
FACScan Calibur Flow Cytometer
Aria II Cell Sorter
This facility provides services outside its institution
This facility provides consulting outside its institution
10/01/2015
10x Genomics
16S Amplicon Sequencing
Assays and Measurements
Consultation
Consultations
Data Analysis
DNA Analysis
Genomics
Genotyping
Genotyping-by-sequencing
Nucleic Acid Extraction
PCR Arrays
Real-time qPCR
RNA analysis
RNA Integrity
RNA Isolation
RNAseq
Sequencing - Capillary Sequencing
Sequencing - DNA Sequencing
Genomics / Genome Analysis and Technologies
University at Albany, LSRB Room 1022
1400 Washington Ave.
Albany, NY 12222 - United States of America
The CFG Molecular Biology Laboratory offers comprehensive research services in molecular biology. Our facilities are equipped with state-of-the-art instrumentation and our experienced staff provides a valuable resource to help our customers, from both academic and commercial laboratories, to meet their research goals and objectives. Our services include standard core services such as DNA sequencing, genotyping, SNP analysis, nucleic acid isolation, and quantitative PCR analysis, as well as customized services.
Major instrumentation in the laboratory includes an Applied Biosystems (AB) 3730XL DNA Analyzer, ABI QuanStudio 12K Flex quantitative PCR system, Agilent Bioanalyzer 2100, Invitrogen Qubit 2.0 fluorometer, NanoDrop 1000 microvolume UV spec, NanoDrop 3300 microvolume fluorometer, Affymetrix and Agilent microarray processing.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/02/2024
10x Genomics
Assays and Measurements
Biochemical Analysis
Bisulfite Conversion
Cell Line Authentication
ChIP-Seq
Computational - Bioinformatics
Data Analysis
Discovery Science
DNA Analysis
DNA Isolation
Genomics
Genotyping
Laser Capture Microdissection
Library Services
Microarray
Nucleic Acid Extraction
OpenArray
PCR Arrays
Real-time qPCR
RNA analysis
RNA Integrity
RNA Isolation
RNAseq
Sequencing - Capillary Sequencing
Sequencing - Next Generation Sequencing (NGS)
Shared Instrumentation Oversight & Maintenance
Single-cell Sequencing
Slide Scanning
Genomics / Genome Analysis and Technologies
Sridar Chittur
Life Sciences Research Building, Rm 1022
1400 Washington Avenue
Albany, NY 12222 - United States of America
RRID:SCR_018262
Next gen sequencing (Illumina, MinIon, Wafergen-Takara, 10x genomics)
DNA microarrays (Affymetrix, Agilent, custom)
This facility provides services outside its institution
This facility provides consulting outside its institution
04/29/2024
2D-PAGE
Antibody Sequencing
Assay Development
Assays and Measurements
Biochemical Analysis
Capillary Electrophoresis
Gel Chromatography
HPLC
Isothermal Titration Calorimetry (ITC)
Lipidomics
Mass Spectrometry
Metabolomics
Phosphoproteomics
Protein Extraction/Purification
Proteomics
RNA analysis
SDS-PAGE
Sequencing - Protein Sequencing
Shared Instrumentation Oversight & Maintenance
Western Blot
Qishan Lin
1400 Washington Ave
ls1085
Albany, NY 12222 - United States of America
RRID:SCR_017695
The RNA Epitranscriptomics and Proteomics Resource (REPR), built upon the existing Proteomics core facility as well as the RNA Institute Mass Spectrometry Center, is a joint venture between the VPR office and the Dean of the College of Arts and Sciences. This specialized laboratory maintains a comprehensive state-of-the-art mass spectrometry research facility to support a wide range of internal and external research projects. The REPR is dedicated to providing high quality mass spectrometry services in a time and cost-effective manner. We are a full-service provider of both small and large molecule laboratory, training and consulting services with value-added solutions. In addition to supporting the university research enterprise, the REPR also serves the pharmaceutical and biotechnology industries, universities, and government labs.
RNA Epitranscriptomics
The RNA epitranscriptomics services include a comprehensive range of custom services such as RNA purification, RNA characterization, and RNA chemical modification analysis using quantitative mass spectrometry.
Proteomics/Mass Spec
The proteomics/mass spectrometry services include comprehensive targeted and un-targeted metabolomics and proteomics, high resolution mass measurement for both large and small molecules, PK/PD study, bio-similar characterization, ADC characterization, and large-scale characterization of proteins and metabolites expressed in health and disease.
This facility provides services outside its institution
This facility provides consulting outside its institution
07/07/2022
Assays and Measurements
Cell Culture
Cell Sorting
Data Analysis
Flow Cytometric Analysis
Microarray
Proteomics
RNA analysis
RNA Integrity
Sridar Chittur
One Discovery drive, CRC319A
Rensselaer, NY 12144 - United States of America
Cell Culture/ Staining/FACS analysis/Cell Sorting
Beckman-coulter ALTRA, BD FACS Aria
BD- LSRII
This facility does not provide services outside its institution
This facility does not consult outside its institution
Assays and Measurements
Cell Culture
Cell Imaging
Confocal Microscopy
Data Analysis
Electronics & Fabrication Shop
Histology
Immunohistochemistry
Microscopy
Multiphoton Microscopy
Optical Imaging
Outreach
Tissue Culture
Imaging (Cell, Molecular, PET, Translational)
Richard Cole
150 New Scotland Avenue
Albany, NY 12208 - United States of America
RRID:SCR_021104
Adherent cells or tissue fragments can be imaged in a variety of methods depending on the question being asked. If drug delivery is required, a perfusion style chamber is used; if gas exchange for the growth media is needed glass bottom Petri dishes can be used; if long term (> 3 days) sterility is needed, there are sealed Rose chambers for imaging. Regardless of what the experimental design, the specimen can be maintained from RT to 50 ºC. Non-destructive image modes i.e. phase contrast, differential interference contrast or polarized light coupled with shutter and filtered illumination provide as close to homeostatic conditions as possible.
Once the images are collected there is an extensive array of image processing & analysis software to assist in data analysis/interpretation. These “tools†range from the simple, contrast manipulation to complex tracing and counting algorithms. There are also different forms of deconvolution available for "removing" the blur from fluorescent specimens, or transmitted light images. If multiple molecules are labeled, co-localization can be determined for either a static image or an image sequence. There are both automatic and semi-automatic 2 and 3-D tracking/montaging algorithms available.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/21/2021
Assays and Measurements
BioBanking
Cell Culture
Cell Imaging
Cell Sorting
Central Laboratory Supply
Confocal Microscopy
DNA Analysis
Flow Cytometric Analysis
Media Preparation
Microarray
Microscopy
PCR Arrays
Real-time qPCR
Stem Cell Procurement
Surgical Services
Tissue Culture
Western Blot
Steve
1 Discovery Drive
Rensselaer, NY 12144 - United States of America
RRID:SCR_017821
NeuraCell is a core facility that makes neural stem cell (NSC) research practical for those wishing to join the field. Our group has over 20 years of experience working with these cells and can now provide you with NSCs, iPS cells, RPE cells and everything you need to grow them. In addition to cells and media, we also provide a range of molecular, cytometry, microscopy and confocal microscopy services. We also provide a consult based characterization service where we can assess how your products or reagents affect stem cell performance and behavior. We are not just limited to stem cells and since we have no in-directs, our pricing is lower than most.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/06/2014
Alphafold
Computational - Application Development
Computational - Bioinformatics
Computational - High Performance Computing
Computational Biology
Computational Chemistry
Hit-to-Lead Optimization
Molecular Dynamics
Molecular Modeling
Peptide Docking
Protein Design
Small Molecule Docking
Virtual Screening
David Johnson
2034 Becker Dr.
Lawrence, KS 66047 - United States of America
RRID:SCR_017890
The University of Kansas Computational Chemical Biology Core (CCB) provides the computational resources and expertise to enhance the productivity of researchers in a variety of fields, including biology, biochemistry, medicinal chemistry, pharmacology, and pharmaceutical chemistry. The CCB has the tools and expertise to perform virtual screening, protein-small molecule docking, binding site prediction, protein modeling and design, prediction of protein stability changes upon mutation, fragment based probe design, molecular dynamics, as well as preparation of presentation graphics.
With the introduction of Alphafold, the CCB has significant expertise in interpreting results, modeling specific ligands and/or alternate states that weren't returned by Alphafold, and virtual screening against Alphafold models, which often are not suitable for a traditional virtual screen. This is accomplished through out specialization in initial hit identification of non-traditional drug targets such as structures lacking pre-formed small molecule binding site or protein-RNA interfaces by offering high-throughput virtual screening via pocket optimization with exemplar screening at protein-protein interfaces and hotspot pharmacophore mimicry of protein-RNA interactions.
We look forward to working with you in any stage of your project, from hypothesis formation, gathering of preliminary data for grant submissions, long-term collaborations, all the way to providing a figure to put the finishing touch on your manuscript.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/10/2024
Global PTM Mapping
HRAM Mass Spectrometry
Label Free Quantitative Proteomics
Labeled Quantitative Proteomics -TMT And Other Labeling
Mass Spectrometry - Proteins And Peptides
Mass Spectrometry - Small Molecules
Nano-LC-MS -complex Mixtures
Phosphoproteomics
Proteomics
Protocol Development
SDS-PAGE
Thermal Protein Profiling - TPP
Mass Spectrometry
Anita Saraf
2034 Becker Drive
Del Shankel Structural Biology Center
Lawrence, KS 66047 - United States of America
RRID:SCR_021857
The Mass Spectrometry and Analytical Proteomics Core (MSAPL) at KU provides a range of mass-spectrometry (MS) based services including small molecule analysis & quantitation, peptide and protein MW determination.
We also provide many MS-based proteomics services and associated bioinformatics including: global proteome IDs (Discovery) and targeted proteomics, post-translational modification mapping, quantitative proteomics (Label-free; TMT; other labels). We also provide consultation for designing proteomics experiments. In addition to that we also provide thermal protein profiling (TPP) .
This facility provides services outside its institution
This facility provides consulting outside its institution
01/31/2024
Nuclear Magnetic Resonance (NMR)
Marimar Lopez
1607 15th street
Troy, NY 12180 - United States of America
http://biotech.rpi.edu/index.php/facilities/magnetic-resonance
RRID:SCR_023491
The Nuclear Magnetic Resonance (NMR) Research Core provides Rensselaer researchers, in addition to academic and industrial users throughout the community, access to state-of-the-art, ultra-high field NMR instrumentation. The diverse capabilities of the facility are enabled with an array of fixed and broadband tunable probes for solution and solid-state NMR, and resonators and high power gradients for diffusion and magnetic resonance microimaging. The NMR core actively supports research programs including structure determination of small molecules, proteins, carbohydrates, nucleic acids, and macromolecular complexes at atomic resolution, quantitative analysis of complex mixtures, metabolomics applications, diffusion analysis, characterization of materials, and MR microimaging.
Expert training and assistance is available in experiment design and/or pulse sequence customization, sample preparation, data acquisition, processing, analysis, and reporting of results (e.g. manuscript and/or grant preparation) in addition to comprehensive fee-for-service support.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/19/2023
Assays and Measurements
Biochemical Analysis
Cell Culture
Gel Chromatography
Molecular Construct Services
Nucleic Acid Extraction
Peptide Synthesis
Protein Extraction/Purification
SDS-PAGE
Spectroscopy
Surface Plasmon Resonance (SPR)
Tissue Culture
Viral Vectors
Western Blot
Dr. Philip Gao
Del Shankel Structural Biology Center
2034 Becker Drive
Lawrence, KS 66047 - United States of America
RRID:SCR_017749
The KU Protein Production Group (PPG) focuses on the cloning, expression and purification of prokaryotic and eukaryotic proteins for KU and other investigators in Kansas and the nation. The laboratory maintains a variety of equipment to support the production of properly folded proteins in quantities suitable for structural studies (X-ray and NMR), functional studies (catalytic or biological), label-free binding studies (SPR) and/or high throughput (HTP) screening studies.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/26/2022
Single Crystal X-ray Pattern Analysis
Structural Biology
Structure Determination
X-Ray Crystallography
Structural Biology
Dr. Scott Lovell
Del Shankel Structural Biology Center
2034 Becker Drive
Lawrence, KS 66047 - United States of America
RRID:SCR_021742
The Protein Structure & X-Ray Crystallography Laboratory (PSXL) is a core laboratory at the University of Kansas that possesses the infrastructure and expertise to perform all aspects of protein and small molecule structure determination using X-ray crystallography. For protein structure determination, high-throughput methods are utilized to move projects rapidly from gene-to-structure.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/05/2021
2D Spectra Acquisition and Interpretation
Data Analysis
Nuclear Magnetic Resonance (NMR)
Spectroscopy
Dr. Justin Douglas
Del Shankel Structural Biology Center
2034 Becker Drive
Lawrence, KS 66047 - United States of America
RRID:SCR_018671
The COBRE Biomolecular NMR Laboratory maintains two high field NMR spectrometers in support of structural and dynamics studies of biomolecules with COBRE and other investigators in Kansas and the region. Its capabilities include determining high resolution structures, biological macromolecules, elucidation and structural mapping of protein-protein, protein-nucleic acid, protein-peptide, protein-drug interactions, and studies of the dynamics of proteins and their complexes in solution. Laboratory staff provide advice, consultation, training, assistance and complete structure elucidation services to investigators. The staff is also responsible for maintenance, upgrades, implementation of new NMR pulse sequences, and assisting local and remote users with technical problems.
This facility provides services outside its institution
This facility provides consulting outside its institution
01/07/2020
Assays and Measurements
Biochemical Analysis
Cell Culture
Cell Imaging
Computational - Biostatistics
Confocal Microscopy
Data Analysis
Flow Cytometric Analysis
Surface Plasmon Resonance (SPR)
Tissue Culture
Anu Roy
2034 Becker Drive
Structural Biology Center (SBC)
Lawrence, KS 66047 - United States of America
RRID:SCR_017752
High throughput screening of large chemical libraries of compounds is a proven way to identify novel chemical entities that target a biological system of interest. KU-HTS is a state- of-the-art facility dedicated to providing exceptional services in advancing drug discovery research initiatives, as well as assistance in preparing grant applications. HTS personnel have extensive experience in executing biochemical, cell-based, siRNA as well as high content screening campaigns against a plethora of target classes. KU-HTS is a fee-for-service facility dedicated to providing academia and nonprofit institutions, as well as the biotech and pharmaceutical industry, with exceptional quality services at the lowest cost structure. We partner with the investigators collaboratively to expedite their drug discovery efforts and serve as a uniquely qualified reference facility with high throughput screening instrument manufacturers as well as reagent kit developers and manufacturers.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/02/2019
Mass Spectrometry
Proteomics
RNA analysis
Sequencing - Protein Sequencing
Marimar Lopez
1346 CBIS
110 8th St.
Troy, NY 12180 - United States of America
The Proteomics Core at RPI prvides services for insiders and outsiders researches.
It has a Bruker Ultraflex III MALDI TOF/TOF ,a Thermo Electron Finnigan TSQ Quantum Ultra,a Thermo Scientific LTQ ORBITRAP XL and a Shimadzu GC/MS.
This facility provides services outside its institution
This facility provides consulting outside its institution
07/01/2016
Molecular Library Services
Jeffrey Aube
2034 Becker Drive
Delbert M. Shankel Structural Biology Center (SBC)
Lawrence, KS 66047 - United States of America
RRID:SCR_017750
The Synthetic Medicinal Chemistry Laboratory is a university-based drug discovery facility with a state-of-the-art infrastructure for pharmaceutical research. The Core provides medicinal, synthetic, and combinatorial chemistry support for Core B (HTS) and COBRE investigators. The support involves the optimization of compounds for potency, and selectivity by the following:
Preparing targeted libraries for screening and
lead optimization
Synthesizing organic compounds on various scales
(from mg to multi-gram)
Assisting COBRE investigators and other
university investigators, if requested, in the
design of novel ligands and in the development
of practical synthetic routes.
This facility provides services outside its institution
This facility does not consult outside its institution
10/17/2011
Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI)
Magnetic Resonance Imaging (MRI)
Marimar Lopez
1650 15th Street
Troy, NY 12180 - United States of America
RRID:SCR_017745
Imaging instrumentation:
7T/13cm horizontal and 14T/9cm vertical bore MRI Scanners, Bruker Ultraflex III MALDI TOF/TOF with imaging capabilities and a small animal Scanco Medical Viva CT40 micro-CT scanner
This facility provides services outside its institution
This facility does not consult outside its institution
12/13/2010
Biochemical Analysis
Spectroscopy
Surface Plasmon Resonance (SPR)
Thermal Analysis
X-ray Diffraction and Scattering
Marimar Lopez
Troy, NY 12180 - United States of America
http://www.rpi.edu/research/biotech/facilities/analytical/index.html
The Analytical Biochemistry Core Facility at RPI is one of the best equipped research facilities in the northeast. This new facility is equipped with state-of-the-art instrumentation.
Instrumentation:Spex® FluoroLog®-Tau -3 Spectrofluorimeter,Jasco 815 Spectrometer with BioLogic stopped-flow,Bruker D8 Discover X-Ray Diffractometer,Bruker Nanostar Small-Angle X-Ray Scattering,Beckman Analytical Ultracentrifuge XL-I,MicroCal VP-Isothermal Titration Calorimeter (VP-ITC),Shimadzu High Pressure Liquid Chromatograph (HPLC,TA DSC Q100,TA TGA Q50,Wyatt Technology DynaPro Titan Batch Dynamic Light Scattering, Analytical Biochemistry Core Facility
Its mission is to provide service and consultation on many different areas of modern analytical biochemistry to both on and off campus investigators.
This facility provides services outside its institution
This facility does not consult outside its institution
Cell Culture
Cell Imaging
Cell Sorting
Flow Cytometric Analysis
Tissue Culture
Marimar Lopez
Troy, NY 12180 - United States of America
http://www.rpi.edu/research/biotech/facilities/cell/index.html
Instrumentation:
BD Sciences FACSAria,BD Sciences LSRII,GE Typhoon Trio+ ,Roche LightCycler 480 RT-PCR ,Amaxa Nucleofector and much more
This facility provides services outside its institution
This facility does not consult outside its institution
Your Email:
Your Phone (optional):
Message:
Facility Details
About This Facility
Services and Equipment
Publications
Awards & Associations
Metadata
Collaboration:
Find Regional Facilities
Other Facilities at this Institution
