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University of Kansas, Lawrence
Del Shankel Structural Biology Center
2034 Becker Drive
Lawrence, KS 66047
United States of Americahttps://ppg.ku.edu/Dr. Philip Gao
Last Updated: 04/26/2022
The KU Protein Production Group (PPG) focuses on the cloning, expression and purification of prokaryotic and eukaryotic proteins for KU and other investigators in Kansas and the nation. The laboratory maintains a variety of equipment to support the production of properly folded proteins in quantities suitable for structural studies (X-ray and NMR), functional studies (catalytic or biological), label-free binding studies (SPR) and/or high throughput (HTP) screening studies.
The lab is specialized in production of challenging proteins (membrane proteins, proteases, ect.) and provides training in any of the techniques it employs, including cloning, mutagenesis, protein expression and protein purification. Access to facilities is also provided for qualified users.
Services are offerred outside of University of Kansas, Lawrence
Consulting is offerred outside of University of Kansas, Lawrence
Incubators(3), thermocyclers(2), 1D, 2D and preparative electrophoresis, centrifuges(4), cell disruptor, automated FPLCs(3), Biacore T200, UV-vis, Nanodrop, relevant small equipment; close association to high-field NMR, analytical proteomics (Mass spec), high-throughput screening, and X-ray crystallography labs. We also have Bioneer’s ExiProgen™, the only fully automated equipment on the market that uses the E coli cell-free lysate for the synthesis from plasmid or PCR amplified DNA.
No publications have been associated with this facility
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https://coremarketplace.org/?FacilityID=249Keywords:
Protein, production, purification, expression, cloning, service, core
Resource Type:
core facility, access service resource, service resource
Citation:
Kansas University at Lawrence Protein Production Group Core Facility (RRID:SCR_017749)
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