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All Facilities >> University of Kansas, Lawrence >> Protein Structure and X-ray Crystallography Laboratory (Structural Biology)
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Del Shankel Structural Biology Center
2034 Becker Drive
Lawrence, KS 66047
United Stateshttps://psxl.ku.edu/cite this facility
Dr. Scott Lovell
Last Updated: 10/05/2021
The Protein Structure & X-Ray Crystallography Laboratory (PSXL) is a core laboratory at the University of Kansas that possesses the infrastructure and expertise to perform all aspects of protein and small molecule structure determination using X-ray crystallography. For protein structure determination, high-throughput methods are utilized to move projects rapidly from gene-to-structure.
Services are offerred outside of University of Kansas, Lawrence
Consulting is offerred outside of University of Kansas, Lawrence
The following equipment is maintained by the PSL:
• Bruker D8 Venture dual source (Cu-Kalpha and Mo-Kalpha) diffractometer, equipped with a Photon III 14 pixel array detector, kappa goniometer and an Oxford 800 cryostream
• Bruker Microstar micro-focus rotating anode X-ray generator equipped with Helios and Helios MX multilayer optics, Apex 2 and Platinum 135 CCD detectors, fixed chi and kappa goniometers and two Oxford cryostreams
• Molecular Dimensions crystallization incubator for low temperature crystallization
• Three refrigerators for sample and buffer storage
• Three Olympus stereo microscopes (one equipped with a CMOS camera for crystal imaging)
• Eppendorf 5414R and Spectrafuge-16M mini centrifuges for concentrating proteins
• Lab Armour waterless bead bath
• Rigaku Actor and Universal pucks for shipping samples to the synchrotron
• Millipore ultrapure water filtration system
• Formulatrix NT8 drop setting robot equipped with plate copy and LCP options for membrane proteins
• Formulatrix Rock Imager 2 (RI2) with UV-VIS imaging, multiwavelength fluorescence imaging capability
• Formulatrix RockMaker software with secure web access
• Nanodrop ND-1000 Spectrophotometer
• Thermo ultralow temperature freezer
Equipment that is nearby and accessible to the PSXL include:
• Two AKTA-Pure FPLC instruments and various columns for protein purification
• AKTA-Explorer FPLC instrument
• Two Infors HT Multitron Stackable Shaker incubators
• Cell sonicator and freezer mill
• BiaCore T200 surface plasmon resonance instrument
• QuantStudio 3 real-time PCR instrument for protein thermal shift measurements
• Two Sorvall centrifuges
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https://coremarketplace.org/?FacilityID=250Keywords:
USEDit, ABRF, protein structure determination, small molecule structure determination
Resource Type:
access service resource, service resource, core facility
Citation:
University of Kansas Lawrence Protein Structure and X-ray Crystallography Laboratory Core Facility (RRID:SCR_021742)
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Facility Details
About This Facility
Services and Equipment
Publications
Awards & Associations
Metadata
Other Facilities at this Institution:
