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University of Kansas, Lawrence
Del Shankel Structural Biology Center
2034 Becker Drive
Lawrence, KS 66047
United States of Americahttp://psf.cobre.ku.eduDr. Scott Lovell
Last Updated: 04/02/2018
The Protein Structure Core Laboratory (PSL), located in the Del Shankel Structural Biology Center building at the University of Kansas, performs or assists investigators with any or all of the following tasks: protein construct design; protein purification; crystallization screening; isolation of single crystals and assessment of crystal quality; X-ray diffraction data collection in-house and/or at a synchrotron; structure solution and refinement; protein model building, structure analysis and preparation of publication-quality graphics. In addition the PSL will provide training of students and/or lab personnel in any or all crystallographic and data analysis techniques available in the PSL. It also provides assistance in the preparation of manuscripts and grant applications dealing with crystallography and structure determination.
The PSL is well suited to facilitate structural biology efforts for researchers. We employ high throughput crystallization methods in climate controlled environments that permit one to screen a large number of crystallization conditions using small volumes of protein sample relative to traditional methods. This increases the chance of obtaining crystals since a greater number of crystallization conditions can be investigated using a single protein sample. Currently, the PSL has a success rate of approximately 70% at obtaining confirmed protein crystals for investigator projects. Adjacent to the crystallization laboratory, we house two rotating anode generators, image plate and CCD detectors and cryo cooling units in the X-ray laboratory that are used regularly for screening and data collection for a variety of protein crystals. These instruments are used by the PSL and the Small-Molecule X-Ray Crystallography Laboratory at KU. Along with our in-house X-ray diffraction capabilities, the PSL maintains collaborations with scientists at the Advanced Photon Source (APS) IMCA-CAT (sector 17) for synchrotron X-ray data collection. Samples are typically shipped to the APS approximately each month. Diffraction data are collected by an IMCA staff member and the data are downloaded via secure transfer to the PSL. Other laboratories located adjacent to the PSL include the Protein Production Group (PPG) and the KU Analytical Proteomics Laboratory, both of which contain equipment that is accessible to the PSL staff.
Services are offerred outside of University of Kansas, Lawrence
Consulting is offerred outside of University of Kansas, Lawrence
The following equipment is maintained by the PSL:
• High brilliance rotating anode X-ray generator (Rigaku RU-H3R); Blue Max Flux (Osmic) confocal optical system; Two theta stage and inverse Phi axis; R-AXIS IV++ imaging plate system; X-stream 2000 cryogenic system
• Molecular Dimensions crystallization incubator for low temperature crystallization and two additional
• Three refrigerators for sample storage
• Two Olympus stereo microscopes (one equipped with a Spot Insight2 CCD camera for crystal imaging)
• Eppendorf 5414R and Spectrafuge-16M mini centrifuges for concentrating protein
• Rigaku Actor and Universal pucks for shipping samples to the synchrotron
• Millipore ultrapure water filtration system
• Formulatrix NT8 drop setting robot equipped with plate copy option
• Formulatrix Rock Imager 2 (RI2) with UV-VIS imaging, multiwavelength fluorescence imaging and trace fluorescence labeling capability
• Formulatrix RockMaker software with web access
Equipment that is nearby and accessible to the PSL include:
• Bruker Microstar micro-focus rotating anode X-ray generator equipped with Helios and Helios MX multilayer optics, Apex 2 and Platinum 135 CCD detectors, fixed chi and kappa goniometers and two Oxford cryostreams (KU Small-Molecule X-Ray Crystallography Laboratory)
• Nanodrop ND-1000 Spectrophotometer for measuring protein concentration
• AKTAxpress, AKTA-Purifier, AKTA-FPLC and AKTA-Pure FPLC instruments and various columns for protein purification (KU Protein Production Group)
• Shaker incubators, cell sonicator and Constant Systems cell disruptor (KU Protein Production Group)
• BiaCore T200 surface plasmon resonance instrument (KU Protein Production Group)
• Ultra-low temperature freezer (Shared Item)
• Two Sorvall centrifuges (KU Protein Production Group)
• Zenobia Fragment Library version 2.2 (KU Protein Production Group)
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