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University of Kansas, Lawrence
Del Shankel Structural Biology Center
2034 Becker Drive
Lawrence, KS 66047
United Stateshttps://psxl.ku.edu/Dr. Scott Lovell
Last Updated: 10/05/2021
The Protein Structure & X-Ray Crystallography Laboratory (PSXL) is a core laboratory at the University of Kansas that possesses the infrastructure and expertise to perform all aspects of protein and small molecule structure determination using X-ray crystallography. For protein structure determination, high-throughput methods are utilized to move projects rapidly from gene-to-structure.
The PSL is well suited to facilitate structural biology efforts for researchers. We employ high throughput crystallization methods in climate controlled environments that permit one to screen a large number of crystallization conditions using small volumes of protein sample relative to traditional methods. This increases the chance of obtaining crystals since a greater number of crystallization conditions can be investigated using a single protein sample. Currently, the PSL has a success rate of approximately 70% at obtaining confirmed protein crystals for investigator projects. Adjacent to the crystallization laboratory, we house two rotating anode generators, image plate and CCD detectors and cryo cooling units in the X-ray laboratory that are used regularly for screening and data collection for a variety of protein crystals. These instruments are used by the PSL and the Small-Molecule X-Ray Crystallography Laboratory at KU. Along with our in-house X-ray diffraction capabilities, the PSL maintains collaborations with scientists at the Advanced Photon Source (APS) IMCA-CAT (sector 17) for synchrotron X-ray data collection. Samples are typically shipped to the APS approximately each month. Diffraction data are collected by an IMCA staff member and the data are downloaded via secure transfer to the PSL. Other laboratories located adjacent to the PSL include the Protein Production Group (PPG) and the KU Analytical Proteomics Laboratory, both of which contain equipment that is accessible to the PSL staff.
Services are offerred outside of University of Kansas, Lawrence
Consulting is offerred outside of University of Kansas, Lawrence
The following equipment is maintained by the PSL:
• Bruker D8 Venture dual source (Cu-Kalpha and Mo-Kalpha) diffractometer, equipped with a Photon III 14 pixel array detector, kappa goniometer and an Oxford 800 cryostream
• Bruker Microstar micro-focus rotating anode X-ray generator equipped with Helios and Helios MX multilayer optics, Apex 2 and Platinum 135 CCD detectors, fixed chi and kappa goniometers and two Oxford cryostreams
• Molecular Dimensions crystallization incubator for low temperature crystallization
• Three refrigerators for sample and buffer storage
• Three Olympus stereo microscopes (one equipped with a CMOS camera for crystal imaging)
• Eppendorf 5414R and Spectrafuge-16M mini centrifuges for concentrating proteins
• Lab Armour waterless bead bath
• Rigaku Actor and Universal pucks for shipping samples to the synchrotron
• Millipore ultrapure water filtration system
• Formulatrix NT8 drop setting robot equipped with plate copy and LCP options for membrane proteins
• Formulatrix Rock Imager 2 (RI2) with UV-VIS imaging, multiwavelength fluorescence imaging capability
• Formulatrix RockMaker software with secure web access
• Nanodrop ND-1000 Spectrophotometer
• Thermo ultralow temperature freezer
Equipment that is nearby and accessible to the PSXL include:
• Two AKTA-Pure FPLC instruments and various columns for protein purification
• AKTA-Explorer FPLC instrument
• Two Infors HT Multitron Stackable Shaker incubators
• Cell sonicator and freezer mill
• BiaCore T200 surface plasmon resonance instrument
• QuantStudio 3 real-time PCR instrument for protein thermal shift measurements
• Two Sorvall centrifuges
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https://coremarketplace.org/?FacilityID=250Keywords:
USEDit, ABRF, protein structure determination, small molecule structure determination
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
University of Kansas Lawrence Protein Structure and X-ray Crystallography Laboratory Core Facility (RRID:SCR_021742)
Facility Details
About This Facility
Services and Equipment
Publications
Associations
Metadata
