The CoreMarketplace: Protein Structure and X-ray Crystallography Laboratory

The Protein Structure & X-Ray Crystallography Laboratory (PSXL) is a core laboratory at the University of Kansas that possesses the infrastructure and expertise to perform all aspects of protein and small molecule structure determination using X-ray crystallography. For protein structure determination, high-throughput methods are utilized to move projects rapidly from gene-to-structure.

Facility Policies

Services are offerred outside of University of Kansas, Lawrence

Consulting is offerred outside of University of Kansas, Lawrence

(To see more facility information, click the in the upper right)

Services offered:

Single Crystal X-ray Pattern Analysis

Structural Biology

Structure Determination

X-Ray Crystallography

Facility Equipment

The following equipment is maintained by the PSL:
• Bruker D8 Venture dual source (Cu-Kalpha and Mo-Kalpha) diffractometer, equipped with a Photon III 14 pixel array detector, kappa goniometer and an Oxford 800 cryostream
• Bruker Microstar micro-focus rotating anode X-ray generator equipped with Helios and Helios MX multilayer optics, Apex 2 and Platinum 135 CCD detectors, fixed chi and kappa goniometers and two Oxford cryostreams
• Molecular Dimensions crystallization incubator for low temperature crystallization
• Three refrigerators for sample and buffer storage
• Three Olympus stereo microscopes (one equipped with a CMOS camera for crystal imaging)
• Eppendorf 5414R and Spectrafuge-16M mini centrifuges for concentrating proteins
• Lab Armour waterless bead bath
• Rigaku Actor and Universal pucks for shipping samples to the synchrotron
• Millipore ultrapure water filtration system
• Formulatrix NT8 drop setting robot equipped with plate copy and LCP options for membrane proteins
• Formulatrix Rock Imager 2 (RI2) with UV-VIS imaging, multiwavelength fluorescence imaging capability
• Formulatrix RockMaker software with secure web access
• Nanodrop ND-1000 Spectrophotometer
• Thermo ultralow temperature freezer

Equipment that is nearby and accessible to the PSXL include:
• Two AKTA-Pure FPLC instruments and various columns for protein purification
• AKTA-Explorer FPLC instrument
• Two Infors HT Multitron Stackable Shaker incubators
• Cell sonicator and freezer mill
• BiaCore T200 surface plasmon resonance instrument
• QuantStudio 3 real-time PCR instrument for protein thermal shift measurements
• Two Sorvall centrifuges