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University of Massachusetts Medical School
BioTech II, Suite 114
373 Plantation Street
Worcester, MA 01605United Stateshttp://big.umassmed.edu
Last Updated: 11/17/2021
The Biomedical Imaging Group is an interdisciplinary team of engineers and scientists, representing the fields of cell biology, physics, mathematics, and computer science, that combine their expertise in areas such as microscopy, data management, lasers, optoelectronics, applied mathematics, image and signal processing, computer graphics, computer vision, statistics, software engineering, and biophysics, to develop imaging approaches for cell biology.
Since its establishment in the early 1980s, it has been a pioneer in high-resolution 3-D imaging using wide-field fluorescence microscopy together with digital image deconvolution, and image visualization and quantitative analysis techniques, especially as applied to the statistical analysis of images to determine co-localization of fluorescence markers inside cells.
Research in this lab is concerned with both the development and the application of light microscopy and imaging in cell biology and biophysics. Some driving problems include imaging the molecular components driving endocytosis and exocytosis/secretion in various cell types, and the imaging of intracellular calcium signaling in excitable cells such as smooth muscle cells, chromaffin cells, and neurons.
As an example of our achievements, we have developed the TESM microscope that combines Total internal reflection fluorescence (TIRF) with Epi-fluorescence Structured illumination to provide new views of trafficking in the near-plasma membrane domain of cells. We have a unique, custom-built, ultra-high-speed microscope system for imaging the dynamics of intracellular calcium channel events, called "calcium sparks", that are being found to regulate an expanding array of cellular processes. We have a two-photon fluorescence microscope for looking deep inside tissue, completing the range from molecule to organism. Areas of ongoing technology research include high-speed, high-resolution imaging of live cells and tissues, novel image processing approaches, high-performance computing, and computer graphics, with an emphasis on developing open-source tools for the research community.
Services are offerred outside of University of Massachusetts Medical School
Consulting is offerred outside of University of Massachusetts Medical School
Zeiss Axio Plan Histology Microscope
Zeiss Axio Observer D1 Motorized Widefield Microscope
Zeiss Axio Observer Z1 for Multi-Well Acquisition
Nikon TE2000 with Yokogawa CSU-10 Spinning Disk Microscope
Zeiss Axiovert 200M FRET Microscope
LaVision BioTec LightSheet UltraMicroscope II
Zeiss LSM 900 with Airyscan 2
TIRF EpiFluorescence Structured Light Microscope (TESM)
Virus Epifluorescence Structured Light Microscope (VESM)
Publications associated with this facility (Click To View):
USEDit, ABRF, fluorescence microscopy, 3D imaging, light microscopy, live cells imaging, tissue imaging, calcium sparks
Resource, service resource, core facility, access service resource
University of Massachusetts Medical School Biomedical Imaging Group Core Facility (RRID:SCR_021201)