The CoreMarketplace: Proteomics and Mass Spectrometry Facility

University of Massachusetts Medical School

222 Maple Ave

Fuller Bldg

Shrewsbury, MA 01545

United States of America

http://www.umassmed.edu/proteomics/index.aspx

cite this facility

Primary Contact:

Scott Shaffer

Last Updated: 04/07/2014

Facility Details

The University of Massachusetts Medical School (UMMS) Proteomics and Mass Spectrometry Facility offers state-of-the-art proteomics and small molecule analyses to UMMS investigators and to the external scientific community at large. Resources include a staff of five and support for study design, sample preparation, sample fractionation, instrumentation, informatics, data interpretation, and publication assistance.

Facility Policies

Services are offerred outside of University of Massachusetts Medical School

Consulting is offerred outside of University of Massachusetts Medical School

Services offered:

Mass Spectrometry

Proteomics

Facility Equipment

1. Thermo Scientific Q-Exactive tandem hybrid instrument with Waters nanoAcquity UPLC for analysis of complex protein digests and quantitative proteomics using HCD fragmentation (ESI LC-MS/MS). A Thermo Accela 1250 UPLC with HTC PAL autosampler is also configured for accurate mass small molecule applications.

2. Thermo Scientific LTQ Orbitrap Velos Pro tandem hybrid instrument with Waters NanoAcquity UPLC for analysis of complex protein digests and whole protein analysis using CID, HCD, and ETD fragmentation techniques (ESI LC-MS/MS).

3. Thermo Scientific LTQ linear ion trap instrument with Thermo Surveyor HPLC for small molecule analysis (ESI LC-MS/MS).

4. Agilent 6520 quadrupole time of flight instrument (Q-TOF) with Agilent Infinity 1260 HPLC for metabolomics and small molecule quantitative applications.

5. Shimadzu Scientific TOF2 matrix-assisted laser desorption ionization tandem time of flight hybrid instrument (MALDI TOF-TOF) for protein and peptide characterization.

6. Waters Q-TOF Premier quadrupole time of flight instrument with Advion Triversa Nanomate robot for automated infusion studies. System configured (via HPLC) for hydrogen-deuterium exchange studies and whole protein applications (ESI LC-MS/MS).

7. Waters Quattro Premier XE triple quadrupole instrument (QQQ) with either Waters Acquity UPLC for small molecule quantitation or Proxeon EasyLC nano HPLC for quantitation of small molecules and peptides (ESI LC-MS/MS).

Additional supporting equipment and software include:
â€¢ 1D gel electrophoresis platforms, scanning and image analysis.

â€¢ Off-line HPLC separations and fraction collection using a Perseptive Biosystems BioCAD Vision.

â€¢ Supporting software platforms include database searching using Mascot (Matrix Science) or Sequest (Thermo Scientific), de novo peptide sequencing using PEAKS (Bioinformatics Solutions), and comparative quantitative proteomics using Scaffold (Proteomics Software), Mascot Distiller (Matrix Science), ProteoIQ (NuSep), and Sieve (Thermo Scientific).