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Advanced Light Microscopy & Image Analysis Core (Imaging (Cell, Molecular, PET, Translational))

New York State Dept. of Health

150 New Scotland Avenue

Albany, NY 12208

United States of America

https://www.wadsworth.org/research/cores/alm

Primary Contact:

Richard Cole

Last Updated: 04/21/2021

Facility RRID

RRID:SCR_021104

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Facility Details

Adherent cells or tissue fragments can be imaged in a variety of methods depending on the question being asked. If drug delivery is required, a perfusion style chamber is used; if gas exchange for the growth media is needed glass bottom Petri dishes can be used; if long term (> 3 days) sterility is needed, there are sealed Rose chambers for imaging. Regardless of what the experimental design, the specimen can be maintained from RT to 50 ºC. Non-destructive image modes i.e. phase contrast, differential interference contrast or polarized light coupled with shutter and filtered illumination provide as close to homeostatic conditions as possible.

Once the images are collected there is an extensive array of image processing & analysis software to assist in data analysis/interpretation. These “tools” range from the simple, contrast manipulation to complex tracing and counting algorithms. There are also different forms of deconvolution available for "removing" the blur from fluorescent specimens, or transmitted light images. If multiple molecules are labeled, co-localization can be determined for either a static image or an image sequence. There are both automatic and semi-automatic 2 and 3-D tracking/montaging algorithms available.

Other Facility Information

Fluorescence imaging:
Multiple labeled biomolecules such as GFP etc. or fluorophore can be imaged in four-dimensions. This can be done over time (5th dimension) for live specimens as well as statically for fixed specimens. Specimens can be as thin as a single cell or as thick as a whole organism (i.e. drosphilia) or anything in between. Conventional two-dimensional imaging can be done at high sensitivity, i.e. low bleaching rates. Three-dimensional imaging can be done in wide-field for relatively thin specimens or by confocal microscopy for thick specimens.

Facility Policies

Services are offerred outside of New York State Dept. of Health

Consulting is offerred outside of New York State Dept. of Health