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All Facilities >> New York State Dept. of Health >> Advanced Light Microscopy & Image Analysis Core (Imaging (Cell, Molecular, PET, Translational))
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New York State Dept. of Health
150 New Scotland Avenue
Albany, NY 12208
United Stateshttps://www.wadsworth.org/research/cores/almRichard Cole
Last Updated: 04/21/2021
Adherent cells or tissue fragments can be imaged in a variety of methods depending on the question being asked. If drug delivery is required, a perfusion style chamber is used; if gas exchange for the growth media is needed glass bottom Petri dishes can be used; if long term (> 3 days) sterility is needed, there are sealed Rose chambers for imaging. Regardless of what the experimental design, the specimen can be maintained from RT to 50 ºC. Non-destructive image modes i.e. phase contrast, differential interference contrast or polarized light coupled with shutter and filtered illumination provide as close to homeostatic conditions as possible.
Once the images are collected there is an extensive array of image processing & analysis software to assist in data analysis/interpretation. These “tools†range from the simple, contrast manipulation to complex tracing and counting algorithms. There are also different forms of deconvolution available for "removing" the blur from fluorescent specimens, or transmitted light images. If multiple molecules are labeled, co-localization can be determined for either a static image or an image sequence. There are both automatic and semi-automatic 2 and 3-D tracking/montaging algorithms available.
Fluorescence imaging:
Multiple labeled biomolecules such as GFP etc. or fluorophore can be imaged in four-dimensions. This can be done over time (5th dimension) for live specimens as well as statically for fixed specimens. Specimens can be as thin as a single cell or as thick as a whole organism (i.e. drosphilia) or anything in between. Conventional two-dimensional imaging can be done at high sensitivity, i.e. low bleaching rates. Three-dimensional imaging can be done in wide-field for relatively thin specimens or by confocal microscopy for thick specimens.
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Consulting is offerred outside of New York State Dept. of Health
Nikon 55i Upright Microscope
Versatile Eclipse 55i clinical microscope offers optical capabilities, unsurpassed ergonomic features and is optimized for digital imaging. Its white LED illumination system provides a homogenous and bright distribution of illumination across the field for optimal viewing and digital imaging, resulting in spectacular, vivid, true-to-life colors. ergonomic features, like the stay-in-position stage handle and tilting/telescoping ergonomic eyepiece tube ensure best viewing posture, enabling long hours of observations to be carried out in comfort. [Product Link]
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Nikon TE300 Inverted Fluorescence Microscope
Advent of the infinity-corrected CFI60 optical system spurred Nikon design engineers to upgrade their research-caliber inverted microscope from the Diaphot 300 to the Eclipse TE300 in 1997. The vastly improved instrument provides longer working distances that accommodate a wide variety of culture vessels and thick specimens, while maintaining high numerical apertures to ensure suitable levels of brightness, imaging contrast, resolution, and sharpness. The paired oculars of the instrument provide a standard 22-millimeter field of view, but like other microscopes in the Eclipse line, the Nikon TE300 is highly modular and provides several options for binocular and trinocular tubes, condensers, stages, and illumination systems. The standard condenser system fabricated for the Nikon TE300 microscope consists of a series of condenser top lenses and a five-position turret assembly with components for phase, differential interference, and Hoffman modulation contrast techniques. Also, Nikon designers patented a video enhancement contrast system for the microscope that features special polarizers and analyzers that enable the visualization of low contrast minutiae that would ordinarily go unnoticed by traditional video imaging methods. [Product Link]
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Olympus IX73 Inverted Microscope
Olympus IX73 inverted microscope has been integrated into our IXplore systems. IXplore Systems are designed to provide solutions-based packages that suit your research application needs. [Product Link]
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Leica SP5 Leica STED Olympus fluorescences dissecting scope,
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https://coremarketplace.org/?FacilityID=116Keywords:
USEDit, Imaging living cells or tissues, Fluorescence imaging, Image analysis
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
New York State Department of Health Wadsworth Center Advanced Light Microscopy and Image Analysis Core Facility (RRID:SCR_021104)
Facility Details
About This Facility
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