Facility Details

The Deep Sequencing Core provides a next-generation sequencing platform for investigators using Massively Parallel Sequencing (MPS), also known as Parallel Sequencing by Synthesis, or "Deep Sequencing". The core analyzes genomic DNA, DNA fractions (e.g. Chromatin Immunoprecipitation), cDNA and RNA samples by generating libraries of small tagged fragments which are simultaneously sequenced in parallel using an Illumina HiSeq 4000 or MiSeq/MiSeq Dx. Deep Sequencing generates a large number of short overlapping sequences as well as quantifying the occurrence of each individual sequence within the library. The Deep Sequencing Core provides assistance with sample preparation and bioinformatics analysis. The Core also provides sequencing on a Pacific Biosciences Sequel II through the PacBio Core Enterprise.