The SDSU Flow Cytometry and Live Imaging Core is a centralized, state-of-the-art shared resource facility housed in five dedicated laboratory rooms (878 sq. feet) within the North Life Sciences building. The Core is overseen by a Faculty Director and a Technical Director. All services are provided on a fee-for-service, hourly recharge basis and are available for BSL-1 and BSL-2 research activities. Major instrumentation includes a BD Biosciences FACSAria™ Fusion in a biosafety cabinet and FACSMelody™ cell sorting systems; two BD® LSR II flow cytometric analyzers; an Amnis® ImageStreamX Mark II imaging flow cytometer; a PerkinElmer In Vivo Imaging System (IVIS); and a MESO QuickPlex® Q 60MM electrochemiluminescence detection platform. The Core provides comprehensive instrumentation, expert technical support, and consultation in experimental design and data analysis that supports high-parameter cellular phenotyping (up to 18 colors), high-speed, multi-population sorting (up to four populations simultaneously) for enrichment or high-purity recovery, 96-well plate single-cell sorting for clonal expansion or downstream single-cell sequencing applications, multi-parameter quantitative imaging (up to six imaging channels), whole-organism fluorescence and bioluminescence imaging, and multiplex biomarker quantification.

The UCSD Cellular and Molecular Medicine Electron Microscopy core provides services in sample preparation and imaging by Transmission Electron Microscopy and Correlative Light and Electron Microscopy.

Core that provides and produces transgenic mice. Services and methodologies include pronuclear microinjection and blastocyst microinjection for creation of new mouse cell lines as well as archival services like embryo and sperm freezing.

Core which provides Diabetes Research Center investigators access to high-throughput sequencing, data acquisition and analysis. Its main services include Sample QC and Processing, Illumina Platform Library Preparation and Sequencing, and Expression microarrays (RNA, microRNA).

Our Facility houses a 600 MHz Bruker Avance III NMR spectrometer fitted with a 1.7mm triple resonance cryoprobe, for analysis of mass limited samples, and a 24 position sample changer for automated data acquisition. Users may collect their own data after suitable training, or have Facility staff do it for them.

The IGM Genomics Center at the University of California, San Diego, is a core research facility overseen by the IGM. Located in Leichtag 172, the IGM Genomics Center provides a variety of services, including sequencing library preparation and sequencing on Illumina\'s MiSeq and NovaSeq 6000 platforms. We support single cell sequencing on the 10X Genomics Chromium Controller. We also process Illumina Infinium Beadchips, which includes a variety of whole-genome genotyping (WGGT) arrays as well as the Infinium MethylationEPIC BeadChip.

Core offers consultation and training to investigators on range of microscopy techniques, including super-resolution, confocal, multi-photon, and advanced fluorescence imaging and image analysis.

The Penn State College of Medicine Mass Spec and Proteomics Facility offers complete sample prep services, methods development, and bioinformatic/biostatistical analyses for discovery and targeted proteomics and quantitative proteomics (Identification & Quantitation w DIA-PASEF , plus targeted MRM/SRM assays) as well as for small molecule/lipid quantitation.

The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps on a fee-for-service basis. Services offered include, cell sorting, analytic cytometry, laser microdissection and hematology.

FCC facility staff will perform all sorting on the FACS ARIA3.

Analytical applications include immuno-phenotyping, cell cycle analysis (DNA content, BrdU), and calcium flux (ratiometric imaging), among others. Additional modalities (FRET, intracellular organelle staining, etc.) can be developed in response to need; please talk to us about your specific application.

The Leica LMD laser capture microdissection microscope has 4X, 6.3X, 10X, 20X, 40X, and 63x objectives, automated motorized scanning and positioning, and remote stylus operated input.

The Hemavet 950 FS is an animal blood cell counter and differential analyzer. The Hemavet allows for a fast and complete five-part white blood cell differential, red blood cell analysis, and platelet analysis.

Biorepository collection is comprised of blood samples, tissues and other biological materials donated by patients, coupled with information about their health, environment, lifestyle, medical care and responses to medical treatment. Biorepository is accredited by College of American Pathologists.

Core provides development and testing of small animal models to be used for pre-clinical pulmonary research. BSL2 facility that allows the assessment of pulmonary function related to airway hyper-responsiveness, ventilation-induced lung injury, postnatal lung development, airway remodeling, chronic inflammation, allergy, environmental exposures and infection. Services offered include oxygen profiling, infection and treatment; ability to deliver, measure and test pre-clinical potential of therapeutic interventions via aerosol; and housing of small animals.

Provides efficient and comprehensive genomic analyses.

The Atomic Force Microscopy Core at Penn State College of Medicine was created in 2023, in part, through an NIH S10 award (1S10OD030279-01A1). The core’s mission is to provide the College of Medicine research community with access to state-of-the-art atomic force microscopy equipment and expertise as a means to advance the strategic research goals of the institution.

Located in Hershey, Pennsylvania.

Zeiss LSM 980 Cryo-Confocal Microscope

300 kV Krios Cryo-TEM for SPA and tomography, Equipped with Volta phase plates, bioquantum energy filter and K3 direct electron detector

200 kV thermionic JEOL 2100 for cryo-imaging, cryo-tomography, and standard imaging of stained grids

Flow Cytometry analysis, Cell sorting, Flow Imaging analysis

Our mission is to facilitate investigators to use multiple modern NMR applications, including structure elucidation of small molecules, triple resonance experiments of biomolecules, fragment-based drug discovery, metabolomic and tissue analyses.

The Penn State Hershey College of Medicine Comparative Medicine Imaging Core provides equipment and expertise for bioluminescence and fluorescence imaging, in-vivo, full-body, 3D anatomical, functional and molecular imaging and MRI.

High-Performance computing cluster housing x86 compute cores, graphical processing unit (GPU) cores, and directly-attached high-speed storage.

The Custom Antibody Biomedical Core Facility at the COM provides end-to-end service for the production and purification of custom antibodies

The Light Microscopy Imaging Core at Penn State College of Medicine provides consultation and training in ultra-high-resolution imaging of cells and tissues in fixed or live states. The core also provides expertise in quantitative image analysis and consultations on microscopy-related research projects.

The Metabolic Phenotyping Core at the Penn State College of Medicine offers equipment for the comprehensive assessment of energy balance and body composition in experimental rodents (mice and rats). Services include training in the use of the equipment, consultation on experiment design, and help with data analysis.

Our mission is to provide high-quality transmission electron microscopy (TEM) services, education, and training for the Penn State Hershey campus, other academic institutions, and private companies at large.

The lab is equipped with high-resolution transmission electron microscopes (JEOL 1400) and full suite of ancillary sample preparation equipment to fit most TEM-related needs. The equipment can provide nanometer scale analysis for biological, chemical and materials science. The TEM facility offers multiple levels of service that correspond with the services and training each user desires:

Project consultation
Education and training for new users
Full-service transmission electron microscopy.
TEM services include sample preparation, fixation, embedding, ultra-microtomy, staining, TEM imaging, immuno-localization (i.e. immunogold EM), and negative staining.

The Zebrafish Functional Genomics Core at the Penn State College of Medicine was established to provide the Penn State research community with a modern, centralized facility for housing, breeding and performing experiments with zebrafish, one of the fastest-growing model systems in biomedical research.
The mission of the core is to provide a physical and intellectual infrastructure for investigators whose research can benefit from use of the zebrafish as a model system.

Nuclear magnetic resonance, known as NMR, uses the magnetic properties of certain nuclei to study molecular structure. A wide variety of information can be gathered using NMR for chemical studies (including structural and conformational determinations) as well as biological studies (including protein and nuclei acid structure and function).

The core facility offers and operates as a full service core by performing crystallization, X-ray diffraction data collection (both in-house and synchrotron sources) processing, phasing, crystallographic refinement, model building, and visualization. The structural data obtained by the core will provide scientists with a wealth of information including but not limited to biological functions, 3D-folding, ligand binding (small molecule or protein), or mutational effect of target macromolecules.

The Behavior Core at Scripps Florida provides an excellent resource for investigators wishing to include behavioral assays in their research programs. It is divided into separate suites to accommodate either mice or rats, and a wide range of behavioral paradigms is available. Behavior Core personnel can assist in all aspects of rodent behavioral testing including design, training, and execution of behavioral tasks as well as data analysis and interpretation.

The Cell Based High-Throughput Screening (CBS) core provides investigators with access to genome-wide collections of cDNAs, microRNA-adapted short hairpin (shRNA) retroviral libraries, and siRNA libraries that can be used to interrogate cellular models of signal transduction pathways and phenotypes. The core has also assembled a variety of smaller focus sets form each larger library as wells as chemical libraries available for cell-based screens.

At the Center for Diabetes and Metabolic Diseases (Metabolic Core), we offer prompt access to validated in vitro and in vivo tests to help advance the metabolic characterization of genetic and pharmacological research models of metabolic diseases, cancer, circadian rhythms, aging, inflammation and more.

We have consolidated analytical and in-vivo laboratories with a number of applications ranging from feeding, sleep, temperature, blood pressure, body composition and metabolic monitoring in mice, to cellular metabolism and in-depth chemical analysis of hormones, nutrients, inflammatory mediators and more.

Additionally, we will assist our users with trainings, experimental design and data analysis as needed and promptly respond to their troubleshooting requirements during experimentation.

The facility provides access to high-speed cell sorting, analytical flow and imaging flow cytometry, and validated antibody panels for immune profiling on the SBP campus in La Jolla. Trained SBP investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use. Trained external users have access during business hours.

Core staff provide technical expertise, hardware and software training, operate the facility‘s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.

Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.

Core provides Cryo-EM services focused mostly on Single Particle Analysis. Housed microscopes include a Titan Krios with Gatan K3 detector.

We support internal and external clients with Cryo-EM services focused mostly on Single Particle Analysis. Our microscopes include a Titan Krios with Gatan K3 detector.

The SBP Viral Vector Core Facility provides state-of-the-art viral vector-based gene/shRNA delivery technology. Its portfolio ranges from lentivirus, retrovirus, adenovirus, adeno-associated virus, zika virus, coronavirus, sindbis virus and vesicular stomatitis virus products as well as customized "The Works" viral vector construction and swapping service packages. There are over 50 ready-to-transduce reporter viruses available for live cell imaging and 2D/3D cell-based assays. For custom virus production, we can achieve high-titer, top quality viral particles suitable for in vitro and in vivo studies. The automated mini-scale preparations of vector plasmids and viruses in 96-well format can immediately allow downstream viral array screening. The core also extends technical expertise in non-viral nanoparticles which includes large extracellular vesicles and exosome purification, analysis and engineering.

The SBP Genomics Core provides end-to-end genomics solutions, from experimental design through sequencing, for academic and industry researchers. Our team offers full-service library preparation, high-accuracy sequencing on the Element Biosciences AVITI24, and access to the latest single-cell, spatial and epigenetic technologies.

Our services include:
- Next generation sequencing: RNA-seq, whole genome, whole exome and targeted sequencing
- Epigenetic profiling: DNA methylation, ATAC-seq, CUT&RUN, CUT&Tag and TIP-ChIP
- Single-cell multiomics: 10x Genomics Chromium X and Illumina PIP-seq
- Spatial transcriptomics and epigenomics: Curio Trekker, Illumina StrataMap, 10x Visium HD and GeoMx DSP
- In situ cell screening: Element Biosciences Cytos multiomic CRISPR and drug screening
- Sample QC, STR cell line authentication and instrument training

The SBP Functional Genomics Core Facility provides the infrastructure for cell-based gain-of-function (ORF, CRISPRa and CRISPRon) and loss-of-functioning (siRN, miRNA, shRNA, CRSIPRko, CRISPRi and CRISPRoff), libraries screening services, starting with assay development and carrying all the way through verification of identified targets. The large scale screens is accomplished via ready-to-use genome-wide or pathway-specific library platforms with choice of arrayed or pooled libraries. The FG core facility is equipped to perform custom CRISPR gene activation, knock-down, knock-out and editing services. We also adopt CRISPR nucleotide base editing technology and offer custom built CRISPR domain libraries. This approach can reveal the specific domain of a target protein interacting with another molecule, such as protein, IncRNA or drug in a native cell cellular environment.

The Sanford Burnham Prebys Proteomics Shared Resource provides state-of-the-art mass spectrometry-based proteomics services including identification, quantification and characterization of proteins and their post-translational modifications. We provide proteomics services to Institute scientists as well as outside non-profit and for-profits organization on a fee-for-service basis.

Academic drug discovery facility with advanced

The Sanford Burnham Prebys Cancer Metabolism Core provides a unique service for the analysis of metabolism, offering both metabolite quantification and stable-isotope tracing methodology, centered around gas chromatography-mass spectrometry (GC-MS). We offer GC-MS analysis of polar metabolites (TCA cycle, glycolysis metabolites and amino acids), short chain fatty acids, fatty acids and cholesterol, sugars and sugar-phosphates. As well as cells, we can also analyze tissues, tumors, serum or culture medium as necessary. We also have available a YSI 2950 analyzer for measurement of glucose, lactate and glutamine in medium samples. We collaborate closely with Core users and can provide in-depth advice on the direction of metabolic experiments, as well as the interpretation of results.

Core facility dedicated to providing a static or dynamic (in vitro/in vivo) imaging services by offering histology and microscopy services and providing support and training to interested researchers. The facility offers a range of light microscopes, related imaging systems and image analysis resources for assisted or independent use (upon training). It also can provide advice on proper sample fixation, processing, embedding of tissues with attention to desired orientation, decalcification of bone tissue, cutting of paraffin blocks, cryosectioning, routine and special stains as well as training in histological techniques.

Flow cytometry core which offers a wide range of routine and custom flow cytometry, mass cytometry, cell sorting services, data analysis, strategic planning for novel assays, user training, and full scale research collaborations.