University of Nebraska Medical Center
Dept. Genetics, Cell BIology and Anatomy
985915 Nebraska Medical Center
Omaha, NE 68198-5915http://www.unmc.edu/genetics/mgecore
Last Updated: 07/02/2010
The UNMC Mouse Genome Engineering Core Facility provides expertise and advice for the experimental design of transgenic or gene knockout experiments, including DNA construct production and genotyping assays. The Core also makes reagents available for the generation of transgene or gene targeting constructs, and performs all experimental aspects, which include pronuclear injection of transgene constructs, generation of recombinant mouse ES cells, blastocyst injection, and embryo transfer surgeries, for the generation or rederivation of genetically manipulated mouse strains. Transgenic founder mice or chimeric animals with targeted alleles are then transferred to the individual investigator for analysis. The availability of the Mouse Genome Engineering Core is pertinent in establishing mouse models, allowing scientific investigators to focus research efforts on biological rather than technological aspects of each project.
The MGE Core also performs cryopreservation of mouse embryos and sperm, IVF, rederivation of cryopreserved germplasm. In addition the Core performs genotyping, transgene and targeting vector engineering.
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