Facility Details

The Advanced Microscopy Core Facility (AMCF) at UNMC houses state-of-the-art imaging technologies ranging from super-resolution (~ 0.120 um to 0.020 um) to microscopic (~ 0.300 um) to mesoscopic (~ 1 um) biomedical imaging. Imaging specialists provide training and/or actively assist researchers collecting images across the diverse array of imaging instrumentation available in the AMCF with competitively-priced usage fees for internal and external facility users.

The Zeiss ELYRA PS.1 is an inverted microscope for super-resolution (SR) structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) including, PhotoActivated Localization Microscopy (PALM) using photo-switchable/convertible fluorescent proteins, Total Internal Reflection Fluorescence (TIRF) and STochastic Optical Reconstruction Microscopy (STORM).

The Zeiss 800 CLSM with Airyscan (Funding: NIH P30 GM106397) is an inverted microscope dramatically increasing conventional confocal image resolution to ~180 nm using Airyscan technology.

The Zeiss 710 LSM (Funding: NIH S10 RR027301) is an inverted microscope supporting most basic imaging applications, multi-channel and spectral, co-localization, live cell, 3D, and time series imaging.

The Zeiss Celldiscoverer 7 (Funding: NIH P30 GM106397) is a widefield imaging system for automated, time-lapse imaging of live samples.

The Zeiss Axioscan 7 (Funding: NIH P20 GM103427) is a high-performance whole slide scanning system for fluorescence, brightfield, and polarization imaging.

The Miltenyi Biotec Ultramicroscope II Light Sheet fluorescence microscope (Funding: NIH S10 OD030486) extends fluorescent imaging into true 3D, large-scale volumetric imaging of intact tissues, organs, and small organisms.

The AMCF also houses several high-end data analysis workstations with premier image analysis software including HALO (Funding: NIH P20 GM103427) and IMARIS (Funding: NIH S10 OD030486) facilitating data rendering, analyses, and presentation options.