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University of Florida
2033 Mowry Road
CGRC 169
Gainesville, FL 32610
United States of Americahttp://www.biotech.ufl.edu/cores/proteomics/Jin Koh
Last Updated: 11/05/2020
ICBR Proteomics & Mass Spectrometry Facility has provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. Here we present our capabilities in proteomics, highlighting differential proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), tandem mass tags (TMT), iodoTMT, Stable isotope labeling with amino acids in cell culture (SILAC), quantification of proteins by label-free LC-MS/MS, and the analysis of protein post-translational modifications (PTM).
We have a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Proteomics & Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.
Services are offerred outside of University of Florida
Consulting is offerred outside of University of Florida
2D Spectra Acquisition and Interpretation
Protein Extraction/Purification
Agilent 1200 Series HPLC Microarray Processor
The Agilent 1200 Series HPLC System is a liquid chromatography analyzer that achieves high levels of sample throughput by the use of either binary or quaternary pumps and interchangeable autosamplers. The high pressure operation and the use of short columns with small diameter media result in fast, high resolution separations. Features include a dual-lamp design and temperature control of diode array detectors. [Product Link]
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SCIEX 4000 QTRAP Mass Spectrometer
Hybrid triple quadrupole linear ion trap mass spectrometer for drug discovery and development, metabolite identification, contaminant testing, proteomic applications, post translationally modified proteins discovery, protein identification, and biomarker validation. [Product Link]
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1260 Agilent Infinity HPLC
4700 Maldi-TOF/TOF AbSciex
Agilent 3100 Offgel Fractionator
Easy-NLC 1000 Liquid Chromatograph
Easy-NLC 1200 System
Ettan Daltsix
Ettan Ipgphor
GE Typhoon Trio + Variable Mode Imager
Investigator Propic Workstation
LTQ Orbitrap XL System
Luminex FLEXMAP 3D
Orbitrap Fusion Tribird
Q Exactive Plus Hybrid Quadruple-Orbitrap
Tiversa Nanomate
TSQ Altis Triple Quadruple Mass Spectrometer
Ultimate 3000 RSLC Nano System
Vanquish Horizon
Other notes: the hybrid quadrupole-Orbitrap mass spectrometer (Q Exactive Plus, Thermo Scientific),
the hybrid ion trap-Orbitrap mass spectrometer (LTQ Orbitrap XL, Thermo Scientific),
a tandem time-of-flight (4700 Proteomics Analyzer, Sciex),
a quadrupole/time-of-flight (QSTAR Elite, Sciex),
a hybrid triple quadrupole-linear ion-trap mass spectrometer (4000 QTRAP, Sciex)
a biomolecule interaction analysis (Biacore 3000)
No publications have been associated with this facility
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https://coremarketplace.org/?FacilityID=651Keywords:
USEDit, differential proteomics, 2D difference gel electrophoresis, isobaric tags, relative quantitation, absolute quantitation, tandem mass tags, iodoTMT, stable isotope labeling, protein quantification
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
University of Florida ICBR Proteomics and Mass Spectrometry Core Facility (RRID:SCR_019151)
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