The Cytometry core employs multiple flow cytometers, from simple entry-level devices to high-end 5-laser, 16-parameter instruments to analyze or sort cells. The lab also offers laser scanning confocal microscope. The microscope has a variety of excitation lines, from near UV to red, and can collect 3 to 4 emission colors with viable cell time lapse and extensive computed parameter capabilities. This includes FRET, FRAP, image stitching, and 3D.reconstruction.
Services are offerred outside of University of Florida
Consulting is offerred outside of University of Florida
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The BD FACSCanto™ II benchtop flow cytometer is a flow cytometer that features reliable performance and accurate results for experiments requiring up to 10 parameters*. Numerous innovations make the system easy to use, powerful, and productive. A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility. And a patented optical design maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay. [Product Link]
The BD FACSCanto™ is a flow cytometry system that performs reliable performance, accuracy, and ease-of-use for clinical diagnostic services. The BD FACSCanto system has 10-color capability, optical enhancements, and a 4-3-3 configuration deliver high sensitivity and resolution for accurate results. [Product Link]
In vivo imaging system that combines two dimensional and three dimensional optical tomography. It can be used for non invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. [Product Link]
The benchtop SH800S cell sorter is a cell sorter that sorts a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective and easier to perform. System software supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. The SH800S is for non-clinical research use only and is a Class 1 laser product. The SH800S is flexible and can support a variety of applications for individual and core labs. For applications in which cross-contamination is a concern, optional e-beam sterilized consumables can be used to replace the sample line and sorting chip. An optional class A2 Level II biosafety cabinet provides protection for personnel and products. The biosafety cabinet was custom designed for the SH800S and tested by The Baker Company to meet international standards. The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection. [Product Link]