University of Florida
2033 Mowry Road
Gainesville, FL 32610http://www.biotech.ufl.edu/cores/proteomics/
Last Updated: 01/01/2016
Proteomics and Mass Spectrometry Applications in Biological and Medical Research
Jin Koh1, Cecilia Silva-Sanchez1, Ran Zheng1, Ning Zhu1, Sixue Chen1, 2, 3
1Proteomics Division, Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida, Gainesville, FL 32610. 2Department of Biology, University of Florida, Gainesville, Florida, 32611 USA, 3Genetics Institute, University of Florida, Gainesville, Florida 32610, USA
Proteomics and mass spectrometry have provided unprecedented tools for fast, accurate, high throughput biomolecular separation and characterization, which are indispensable towards understanding the biological and medical systems. Studying at the protein level allows researchers to investigate how proteins, their dynamics, modifications, and interactions affect cellular processes and how cellular processes and environment affect proteins. The mission of our facility is to provide excellent service and training in proteomics and mass spectrometry. We present our capabilities in proteomics, highlighting quantitative proteomics, which include two-dimensional difference gel electrophoresis (2D-DIGE), isobaric tags for relative and absolute quantitation (iTRAQ), quantification of proteins by label-free LC-MS/MS, and multiple reaction monitoring (MRM)/parallel reaction monitoring (PRM) as well as the analysis of protein post translational modifications (PTM).
Along with the capacity of separating thousands of proteins and characterizing differential protein expression, we have a suite of state-of-the-art mass spectrometers available for biomedical sciences and advanced technology research, including the hybrid quadrupole-Orbitrap mass spectrometer (Q Exactive Plus, Thermo Scientific), the hybrid ion trap-Orbitrap mass spectrometer (LTQ Orbitrap XL, Thermo Scientific), a tandem time-of-flight (4700 Proteomics Analyzer, Sciex), a quadrupole/time-of-flight (QSTAR Elite, Sciex), a hybrid triple quadrupole-linear ion-trap mass spectrometer (4000 QTRAP, Sciex). These instruments are mainly used for protein identification, posttranslational modification characterization, and protein expression analysis. Our facility also provides biomolecule interaction analysis using Biacore 3000. Proteomics and mass spectrometry are useful in large-scale survey of proteome for hypothesis generation as well as in detailed analysis of target proteins for hypothesis testing. Our services also include accurate molecular weight analysis, MRM-based protein screening, PRM, targeted metabolite profiling, and protein bioinformatics. To ensure success and maximize productivity, the facility offers education, consultation, data processing and reporting, and support of grant application.
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