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University of Florida
2033 Mowry Road
CGRC 178
Gainesville, FL 32610
United States of Americahttps://biotech.ufl.edu/gene-expression-genotyping/cite this facility
Edith "Angel" Sampson
Last Updated: 03/05/2021
The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on Single Cell RNA-Seq, RNA-Seq, 16s metagenomics and Affymetrix gene expression arrays. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the Agilent Bravo robot. QuantiGene RNA Assays measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off ddPCR System can facilitate expanded analysis of single cells. The Genotyping services include fragment analysis using AB3730 (96 capillary technology), development of microsatellite libraries using Illumina sequence data, and genotyping using mouse tails or ear punches. These up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research.
Services are offerred outside of University of Florida
Consulting is offerred outside of University of Florida
Bio-Rad CFX96 Real-Time PCR Detection System
The CFX96 Touch System is a real-time PCR detection system that combines optical technology with temperature control. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, thermal cycler performance, unrivaled stand-alone functionality, and easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. [Product Link]
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Nanodrop 8000 Spectrophotometer
Microvolume UV Vis spectrophotometer for nucleic acid and protein quantification that can measure multiple samples using 1 to 2 uL of samples. It can analyze spectrum of 220nm to 750nm. [Product Link]
Citation IDs: 1337371
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10x Genomics (Chromium)
Agilent 2100 Bioanalyzer
Agilent Bravo Platform
Bio-Rad QX200 Digital PCR System
GeneChip Hybridization Oven 640
GeneChip Scanner 3000 7G
Thermo Fisher 3730XL DNA Analyzer
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https://coremarketplace.org/?FacilityID=647Institution
Institution ROR ID: https://ror.org/02y3ad647Keywords:
USEDit, gene expression, genotyping, single cell RNAseq, PCR, qPCR, analysis, ABRF
Resource Type:
access service resource, service resource, core facility
Citation:
University of Florida ICBR Gene Expression and Genotyping Core Facility (RRID:SCR_019145)
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Facility Details
About This Facility
Services and Equipment
Publications
Awards & Associations
Metadata
Other Facilities at this Institution:
