The CoreMarketplace: UF ICBR Electron Microscopy

ICBR Electron Microscopy supports researchers in visualizing microscopic structures through imaging projects and user training. We strive to aid scientists in solving problems and to help push their research forward by providing the tools needed for successful publication and grant applications. Major service categories include: room temperature and cryo transmission and scanning electron microscopy, confocal laser scanning microscopy, sample preparation and user training.

Facility Policies

Services are offerred outside of University of Florida

Consulting is offerred outside of University of Florida

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Services offered:

Confocal Microscopy

Cryo-Electron Microscopy

Electron Microscopy

High-Pressure Freezing

Immuno Electron Microscopy

Microscopy

Sample Preparation

Scanning Electron Microscopy

Transmission Electron Microscopy

Facility Equipment

Tecnai G2 F20 TWIN 200 kV / FEG TEM
Tecnai G2 Spirit TWIN TEM 120 kV
HITACHI SU5000 SCHOTTKY FIELD-EMISSION SEM
ZEISS CONFOCAL LSM800 MICROSCOPE

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Publications

Publications associated with this facility (Click To View):

1.) Bennett A, Hull JA, Mietzsch M, Bhattacharya N, Chipman P, McKenna R (2024 Nov 29). Maize Streak Virus: Single and Gemini Capsid Architecture Viruses, 16(12), 1861. . PMCID: 11680415.

2.) Mietzsch M, Kamat M, Basso K, Chipman P, Huiskonen JT, McKenna R (2024 Nov 12). Structural characterization and epitope mapping of the AAVX affinity purification ligand Molecular Therapy. Methods & Clinical Development, 32(4), 101377. . PMCID: 11638594.

3.) Ali S, Mastantuono F, Orozco-Torres A, Barua N, Zhou X, Wu L, He M (2024 Nov 3). High-throughput Generation of Collagen Microbeads for 3D Cell Culture and Extracellular Vesicle Production bioRxiv, (), 2024.11.03.621748. . PMCID: 11566015.

4.) Ray AP, Jin B, Eddy MT (2024 Oct 17). The conformational equilibria of a human GPCR compared between lipid vesicles and aqueous solutions by integrative 19F-NMR bioRxiv, (), 2024.10.14.618237. . PMCID: 11507675.

5.) Varholick JA, Thermolice J, Godinez G, Dos Santos V, Kondapaneni R, Maden M (2024 Oct 10). Older spiny mice (Acomys cahirinus) have delayed and spatially heterogenous ear wound regeneration Biology Open, 13(10), bio060565. . PMCID: 11554262.

6.) Littrell CA, Takacs GP, Sankara CS, Sherman A, Rubach KA, Garcia JS, Bell CA, Lnu T, Harrison JK, Zhang F (2024 Oct). Systemically targeting monocytic myeloid-derived suppressor cells using dendrimers and their cell-level biodistribution kinetics. Journal of controlled release : official journal of the Controlled Release Society, 374(), 181-193. . PMID: 39103055.

7.) Ligocki AP, Vinson AV, Yachnis AT, Dunn WA Jr, Smith DE, Scott EA, Alvarez-Castanon JV, Baez Montalvo DE, Frisone OG, Brown GA, Pessa JE, Scott EW (2024 Sep 4). Cerebrospinal fluid flow extends to peripheral nerves further unifying the nervous system Science Advances, 10(36), eadn3259. . PMCID: 11373606.

8.) Brinley AR, Conner PJ, Yu F, Sarkhosh A, Liu T (2024 Aug 9). Morphological and genetic characterization of the muscadine fruit abscission zone Horticulture Research, 11(10), uhae227. . PMCID: 11480701.

9.) Gulkis M, Luo M, Chipman P, Mietzsch M, Söderlund-Venermo M, Bennett A, McKenna R (2024 Aug 6). Structural Characterization of Human Bufavirus 1: Receptor Binding and Endosomal pH-Induced Changes Viruses, 16(8), 1258. . PMCID: 11360561.

10.) Pipis N, Stewart KA, Tabatabaei M, Williams LN, Allen JB (2024 Jul 23). Exploring the Fibrous Nature of Single-Stranded DNA-Collagen Complexes: Nanostructural Observations and Physicochemical Insights. ACS omega, 9(29), 32052-32058. . PMID: 39072094.

11.) Mietzsch M, Kailasan S, Bennett A, Chipman P, Fane B, Huiskonen JT, Clarke IN, McKenna R (2024 Jul 9). The Structure of Spiroplasma Virus 4: Exploring the Capsid Diversity of the Microviridae Viruses, 16(7), 1103. . PMCID: 11281519.

12.) Beliakoff RE, Gonzalez CF, Lorca GL (2024 May 28). Bile promotes Lactobacillus johnsonii N6.2 extracellular vesicle production with conserved immunomodulatory properties. Scientific reports, 14(1), 12272. . PMID: 38806562.

13.) Mietzsch M, Nelson AR, Hsi J, Zachary J, Potts L, Chipman P, Ghanem M, Khandekar N, Alexander IE, Logan GJ, Huiskonen JT, McKenna R (2024 May 3). Structural characterization of antibody-responses from Zolgensma treatment provides the blueprint for the engineering of an AAV capsid suitable for redosing. bioRxiv : the preprint server for biology, (), . . PMID: 38746165.

14.) Smith ED, McKenna R, Mietzsch M, Borchelt DR, Prokop S, Chakrabarty P (2024 Apr 15). Hyperacetylation mimetics within the tau filament core inhibits prion-like propagation of misfolded tau bioRxiv, (), 2024.04.12.589253. . PMCID: 11042196.

15.) Poole B, Oshins R, Huo Z, Aranyos A, West J, Duarte S, Clark VC, Beduschi T, Zarrinpar A, Brantly M, Khodayari N (2024 Jan 29). Sirtuin3 promotes the degradation of hepatic Z alpha-1 antitrypsin through lipophagy Hepatology Communications, 8(2), e0370. . PMCID: 10830086.

16.) Kalkreuter E, Kautsar SA, Yang D, Teijaro CN, Bader CD, Fluegel LL, Davis CM, Simpson JR, Steele AD, Gui C, Meng S, Li G, Viehrig K, Ye F, Su P, Kiefer AF, Lauterbach L, Nichols A, Cepeda AJ, Yan W, Fan B, Jiang Y, Adhikari A, Zheng CJ, Shen B (2023 Dec 15). The Natural Products Discovery Center: Release of the First 8490 Sequenced Strains for Exploring Actinobacteria Biosynthetic Diversity bioRxiv, (), 2023.12.14.571759. . PMCID: 10760148.

17.) Hsi J, Mietzsch M, Chipman P, Afione S, Zeher A, Huang R, Chiorini J, McKenna R (2023 Oct 31). Structural and antigenic characterization of the avian adeno-associated virus capsid. Journal of virology, 97(10), e0078023. . PMID: 37702486.

18.) Velez M, Mietzsch M, Hsi J, Bell L, Chipman P, Fu X, McKenna R (2023 Aug 24). Structural Characterization of Canine Minute Virus, Rat and Porcine Bocavirus Viruses, 15(9), 1799. . PMCID: 10534443.

19.) Stratton CE, Reisinger LS, Behringer DC, Reinke AW, Bojko J (2023 Jul). Alternosema astaquatica n. sp. (Microsporidia: Enterocytozoonida), a systemic parasite of the crayfish Faxonius virilis. Journal of invertebrate pathology, 199(), 107948. doi: 10.1016/j.jip.2023.107948. PMID: 37276935.

20.) da Silva DR, Gonzalez CF, Lorca GL (2023 Jul). Internalization of extracellular vesicles from Lactobacillus johnsonii N6.2 elicit an RNA sensory response in human pancreatic cell lines. Journal of extracellular biology, 2(7), . . PMID: 37720361.

21.) Pénzes JJ, Pham HT, Chipman P, Smith EW, McKenna R, Tijssen P (2023 Jun 14). Bipartite genome and structural organization of the parvovirus Acheta domesticus segmented densovirus Nature Communications, 14(), 3515. . PMCID: 10267136.

22.) Becker MW, Peters LD, Myint T, Smurlick D, Powell A, Brusko TM, Phelps EA (2023 Jun 2). Immune engineered extracellular vesicles to modulate T cell activation in the context of type 1 diabetes. Science advances, 9(22), eadg1082. . PMID: 37267353.

23.) Kher L, Kelley K, Santoro D (2023 Feb 13). Ultrastructural Analysis of Differences in the Growth and Maturation of Staphylococcus pseudintermedius Biofilm on Biotic and Abiotic Surfaces. Microbiology spectrum, 11(2), e0357722. . PMID: 36779719.

24.) Paterno G, Bell BM, Riley-DiPaolo A, LaVoie MJ, Giasson BI (2023). Polymerization of recombinant tau core fragments in vitro and seeding studies in cultured cells. Frontiers in neuroscience, 17(), 1268360. . PMID: 38161790.

25.) Lakshmanan R, Mietzsch M, Jimenez Ybargollin A, Chipman P, Fu X, Qiu J, Söderlund-Venermo M, McKenna R (2022 Oct 9). Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait Viruses, 14(10), 2219. . PMCID: 9612331.

26.) Bojko J, Walters E, Burgess A, Behringer DC (2022 Oct). Rediscovering "Baculovirus-A" (Johnson, 1976): The complete genome of 'Callinectes sapidus nudivirus'. Journal of invertebrate pathology, 194(), 107822. doi: 10.1016/j.jip.2022.107822. PMID: 36030880.

27.) Kher L, Santoro D, Kelley K, Gibson D, Schultz G (2022 Apr). Effect of Nanosulfur Against Multidrug-Resistant Staphylococcus pseudintermedius and Pseudomonas aeruginosa. Applied microbiology and biotechnology, 106(8), 3201-3213. doi: 10.1007/s00253-022-11872-8. PMID: 35384449.

28.) Stratton CE, Reisinger LS, Behringer DC, Bojko J (2022 Mar 17). Revising the Freshwater Thelohania to Astathelohania gen. et comb. nov., and Description of Two New Species Microorganisms, 10(3), 636. . PMCID: 8951847.

29.) Stratton CE, Reisinger LS, Behringer DC, Bojko J (2022 Mar 17). Revising the Freshwater Thelohania to Astathelohania gen. et comb. nov., and Description of Two New Species. Microorganisms, 10(3), . . PMID: 35336214.

30.) Emmanuel SN, Smith JK, Hsi J, Tseng YS, Kaplan M, Mietzsch M, Chipman P, Asokan A, McKenna R, Agbandje-McKenna M (2022 Feb 9). Structurally Mapping Antigenic Epitopes of Adeno-associated Virus 9: Development of Antibody Escape Variants Journal of Virology, 96(3), e01251-21. . PMCID: 8827038.

31.) Cowart JR, Collins DM, Stanton DL, van der Horst G, Larkin IV (2022 Feb). Morphometric and structural analysis of Florida manatee spermatozoa. Anatomical record (Hoboken, N.J. : 2007), 305(2), 446-461. doi: 10.1002/ar.24645. PMID: 33890720.

32.) Lednicky JA, Tagliamonte MS, White SK, Elbadry MA, Alam MM, Stephenson CJ, Bonny TS, Loeb JC, Telisma T, Chavannes S, Ostrov DA, Mavian C, Beau De Rochars VM, Salemi M, Morris JG Jr (2021 Dec). Independent infections of porcine deltacoronavirus among Haitian children. Nature, 600(7887), 133-137. doi: 10.1038/s41586-021-04111-z. PMID: 34789872.

33.) Mietzsch M, Yu JC, Hsi J, Chipman P, Broecker F, Fuming Z, Linhardt RJ, Seeberger PH, Heilbronn R, McKenna R, Agbandje-McKenna M (2021 Nov 9). Structural Study of Aavrh.10 Receptor and Antibody Interactions Journal of Virology, 95(23), e01249-21. . PMCID: 8577363.

34.) Hull JA, Mietzsch M, Chipman P, Strugatsky D, McKenna R (2021 Oct 6). Structural characterization of an envelope-associated adeno-associated virus type 2 capsid Virology, 565(), 22-28. . PMCID: 9911311.

35.) Penzes JJ, Chipman P, Bhattacharya N, Zeher A, Huang R, McKenna R, Agbandje-McKenna M (2021 Sep 9). Adeno-associated Virus 9 Structural Rearrangements Induced by Endosomal Trafficking pH and Glycan Attachment. Journal of virology, 95(19), e0084321. doi: 10.1128/JVI.00843-21. PMID: 34260280.

36.) Pénzes JJ, Pham HT, Chipman P, Bhattacharya N, McKenna R, Agbandje-McKenna M, Tijssen P (2020 Aug 18). Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution. Proceedings of the National Academy of Sciences of the United States of America, 117(33), 20211-20222. . PMID: 32747554.

37.) Mietzsch M, Barnes C, Hull JA, Chipman P, Xie J, Bhattacharya N, Sousa D, McKenna R, Gao G, Agbandje-McKenna M (2020 Feb 28). Comparative Analysis of the Capsid Structures of AAVrh.10, AAVrh.39, and AAV8. Journal of virology, 94(6), . . PMID: 31826994.

38.) Mietzsch M, Kailasan S, Garrison J, Ilyas M, Chipman P, Kantola K, Janssen ME, Spear J, Sousa D, McKenna R, Brown K, Söderlund-Venermo M, Baker T, Agbandje-McKenna M (2017 Jun 1). Structural Insights into Human Bocaparvoviruses. Journal of virology, 91(11), . . PMID: 28331084.

39.) Hoes I, Van Dongen W, Lemière F, Esmans EL, Van Bockstaele D, Berneman ZN (2000 Oct 1). Comparison between capillary and nano liquid chromatography-electrospray mass spectrometry for the analysis of minor DNA-melphalan adducts. Journal of chromatography. B, Biomedical sciences and applications, 748(1), 197-212. . PMID: 11092599.

40.) Borzunov EE, Perepelitsia NP, Shevchenko OA (1977 Sep-Oct). [Technology of chloditan tablets]. Farmatsevtychnyi zhurnal, (5), 90-1. . PMID: 590489.

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Associations

ABRF

Association of Biomolecular Resource Facilities

SEASR

Southeastern Association of Shared Resources

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Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=648
https://coremarketplace.org/RRID:SCR_019146

Institution

Institution ROR ID: https://ror.org/02y3ad647

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, electron microscopy, scanning electron microscopy, cryo transmission, confocal laser scanning microscopy, sample preparation, training, ABRF, MSA, Microscopy Society of America

Resource Type:

training resource, core facility, service resource, access service resource

Citation:

University of Florida ICBR Electron Microscopy Core Facility (RRID:SCR_019146)

UF ICBR Electron Microscopy

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Facility Details

Facility Policies

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Services offered:

Facility Equipment

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Publications

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Associations

ABRF

SEASR

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Facility Profile QR Code (links to this profile)

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UF ICBR Electron Microscopy

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