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All Facilities >> University of Colorado Boulder >> BioFrontiers Advanced Light Microscopy Core (Imaging (Cell, Molecular, PET, Translational))
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Joe Dragavon
Last Updated: 03/25/2025
The BioFrontiers Advanced Light Microscopy Core is a user facility designed to advance biological discovery through quantitative microscopy techniques. The facility is open all researchers from academia, national labs and industry. Current demands in biological research require the capability to observe cellular and sub-cellular events and phenomena both punctually as well as over time. The BioFrontiers Advanced Light Microscopy Core is capable to meet these imaging needs. The low signal levels that result from biologically important processes, as well as the spatio-temporal dynamic nature of these processes (3D; millisecond timescales; longitudinal observation), necessitates the use of versatile, reconfigurable, high-speed, and high-sensitivity imaging technologies. Multiple imaging technologies are housed within the BioFrontiers Advanced Light Microscopy Core that can meet these rigorous demands, ranging from conventional widefield to state-of-the-art Super Resolution/localization microscopies.
Services are offerred outside of University of Colorado Boulder
Consulting is offerred outside of University of Colorado Boulder
Bruker TruLive3D Imager Light-Sheet Microscope
Dual sided illumination light-sheet microscope that is optimized for 3D multi-sample imaging of live specimens in their 3D environment. It is especially suited for multi position imaging of small embryos, 3D spheroids, oocytes and more, enabling time lapse, high throughput imaging experiments. [Product Link]
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Nikon A1R Confocal Laser Scanning Microscope
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
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Bitplane Imaris Workstation
Imaris multidimentional image analysis
Citation IDs: RRID:SCR_020317
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Evident Evident VS200 Slide Scanner
Slide Scanner
Citation IDs: RRID:SCR_020317
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Nikon Nikon AXR Laser Scanning Confocal
Laser Scanning Confocal
Citation IDs: RRID:SCR_020317
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Nikon Nikon AXR+NSPARC Laser Scanning Confocal
Laser Scanning Confocal
Citation IDs: RRID:SCR_020317
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Nikon Nikon NSTORM and TIRF
Single Molecule Localization Microscopy and TIRF
Citation IDs: RRID:SCR_020317
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Nikon Nikon Spinning Disc Confocal with Yokogawa CSU X1
Spinning Disc Confocal
Citation IDs: RRID:SCR_020317
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Revvity Revvity IVIS Lumina III
Whole animal imager
Citation IDs: RRID:SCR_020317
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Revvity Revvity Opera Phenix
High Throughput High Content Screening
Citation IDs: RRID:SCR_020317
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Nikon A1R Laser Scanning Confocal Microscope
Nikon Spinning Disc Confocal Microscope
Nikon NSTORM TIRF Microscope
Nikon Ti-E Widefield Microscope
PerkinElmer Opera Phenix High Throughput Screening System
Molecular Devices ImageXpress
Imaris 3D image analysis software
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https://coremarketplace.org/?FacilityID=1008Institution
Institution ROR ID: https://ror.org/02ttsq026Keywords:
Quantitative microscopy, imaging technology, microscope, laser scanning, confocal microscopy, fluorescence microscopy, analysis, ABRF
Resource Type:
core facility, service resource, access service resource
Citation:
Colorado University Boulder BioFrontiers Advanced Light Microscopy Core Facility (RRID:SCR_018302)
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