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All Facilities >> Stanford University >> CSIF: Cell Sciences Imaging Facility
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Stanford University
279 Campus Drive
Beckman Center, B050
Stanford, CA 94305
http://microscopy.stanford.eduJon Mulholland
Last Updated: 10/14/2011
The Cell Sciences Imaging Facility (CSIF) provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells and tissue. The facility offers sophisticated and demanding microscopy techniques (e.g. FRET, FRAP, 2-photon live cell imaging, photo-activation and uncaging, array tomography, immuno-electron microscopy, high pressure freezing...) to Stanford University and industry researchers. The CSIF is organized into three interdependent imaging labs: the Fluorescence Microscopy Core (FMC) which houses multi-photon, confocal and deconvolution microscopes and image analysis software, the Electron Microscopy Core (EMC), which houses high resolution scanning and transmission electron microscopes and the Array Tomography Core (ATC) that provides complete array tomography services. The CSIF, in addition to its Director, Jon Mulholland, is staffed by four research technicians that have expertise and training in electron and light microscopy.
Services are offerred outside of Stanford University
MICROSCOPES:
2-photon/confocal microscope (Leica SP5, upright, 2-photon, environmental control, live cell imaging system)
Confocal microscope (Leica SP2 AOBS, inverted)
Confocal microscope (Zeiss LSM510 with 2-photon laser, environmental control, inverted)
Widefield deconvolution microscope (API Delta Vision RT system, stage and objective heater)
Automated fluorescence array tomography microscope (Zeiss Axioimager Z1)
Transmission electron microscope (Jeol, 1400-TEM) with CCD camera, rt tomography holder, SerialEM automation
Transmission electron microscope (Jeol, 1230-TEM) with CCD cameras
Field emission scanning electron microscope (Zeiss, Sigma FEG-SEM)
Environmental scanning electron microscope (Hitachi, S-3400N VP-SEM)
Histology microscopes with color camera (Leica DM1000, DM2000)
EQUIPMENT:
4 Ultramicrotomes (Leica Ultracut and cryo ultracut)
High pressure freezer w/ rapid specimen exchange (Leica EMPact)
Knife maker (includes 1 glass strip)
Vacuum (Carbon) evaporator
Sputter coater
Microwave oven processing
Cryo-fixing Equip: Plunge/block freezer
Freeze substitution machine
Critical Point Dryer (CPD)
Imaging/Scanner/printer/CD burner
Specimen processing reagent kit
Volume rendering, image processing, 64bit, work stations (2x) running Leica and Zeiss software as well as Volocity software
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Facility Details
About This Facility
Services and Equipment
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