The CoreMarketplace: Advanced BioImaging Facility (ABIF)

The McGill University Life Sciences Complex Advanced BioImaging Facility (ABIF) offers more than a dozen state-of-the-art light microscopy platforms. With tremendous expertise our in-depth training and expert advice will accelerate any light microscopy research project. Comprehensive consultations, sample preparation advice, ongoing support, image processing, image analysis and preparation of data for publication all contribute to using researchers time effectively, generating high quality quantitative data and elevating research projects to the next level. The facility has expertise in many areas of cellular imaging including, but not limited to, live cell imaging, FRAP, FLIM, FRET, FCS, image correlation spectroscopy, TIRF, spectral imaging, high content screening, quantitative image analysis, and custom image analysis. More recently we have been moving into AI/ML/DL for image analysis and generating custom ImageJ workflows for users.

Facility Policies

Services are offerred outside of McGill University

Consulting is offerred outside of McGill University

(To see more facility information, click the in the upper right)

Services offered:

Multiphoton Microscopy

Optical Imaging

Outreach

Facility Equipment

1. Widefield microscopy with environmental control, large field of view CCD camera, multipoint, tiling, multicolour fluorescence for live cell imaging.
2. Laser Scanning Confocal Microscopy, environmental control, spectral imaging, fluorescence lifetime imaging (FLIM), Photomanipulation, FRET imaging, fluorescence correlation spectroscopy (FCS), raster image correlation spectroscopy (RICS), multipoint, tiling, live cell microscopy.
3) Multiphoton microscopy: a) upright confocal microscope with combined electrophysiology, b) intra-vital microscope for mouse imaging.
4) Zeiss Z1 light sheet microscope, environmental control, multi-colour imaging, fixed cleared tissue, live tissue, live organism, samples up to 2x2 mm.
5) Combined total internal reflection fluorescence (TIRF) microscopy and spinning disk confocal. Environmental control, two colour simultaneous imaging, image correlation microscopy to measure protein dynamics and interactions.
6) High end image processing and analysis workstations with cutting-edge software - Imaris, MetaMorph/MetaXpress, Autoquant, ImageJ/FIJI.