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University of Cincinnati
3125 Eden Ave
Vontz Center
Cincinnati, OH 45267-0548
United States of Americahttp://med.uc.edu/cancerbiology/research/proteomicsKen Greis
Last Updated: 01/02/2018
The UC Proteomics Laboratory (UCPL) is fully equipped with the protein separation and mass spectrometry equipment as well as the trained personnel to perform comparative analyses of complex protein mixtures and to identify the differentially-expressed proteins. Proteomics laboratory personnel also offer guidance to the research community regarding the feasibility of a given approach and/or advice on proteomics experimental design to enhance the probability of success. Please contact the lab director to set up a consultation.
The research expertise in the UC Proteomics Laboratory provides the identification of differentially-expressed proteins and the characterization of protein modifications (e.g. phospho, ubiquitin, glyco). Furthermore various heavy isotope tagging (e.g. iTRAQ, SILAC) and label-free methods are used in the laboratory such that the mass spectrometer can be used to both identify the proteins and quantify the changes in abundance. Additional research oriented projects include methods to quantify global changes in phosphorylation from cell and tissues and mass spectrometry-based methods for enzyme assays and high-throughput inhibitor screening.
Our services and reporting can be customized to assist business entities with specific needs. We currently act as the QA service for several vendors who supply proteins and peptides to customers.
Services are offerred outside of University of Cincinnati
Consulting is offerred outside of University of Cincinnati
(1) Sciex 4800 MALDI-TOF/TOF MS
(2) Sciex 5600+ TripleTOF (quadrupole-tof ms) w/Eksigent NanoLC Ultra
(3) Ettan IsoDalt 12 large format 2D gel system
(4) ProteinPilot and Mascot multiprocessor search servers.
No publications have been associated with this facility
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