AMDL is a clinical CAP/CLIA accredited molecular diagnostics laboratory at the Princess Margaret Cancer Centre. Supports clinical trials and translational research projects requiring clinical-grade (CAP/CLIA) molecular testing at Princess Margaret Cancer Centre. AMDL works closely with Molecular Diagnostics lab in Toronto General Hospital to transition protocols into clinical tests.

Core provides specialized, high-dimensional immune profiling for clinical and translational studies. It enables researchers to analyze immune responses using advanced flow cytometry, CyTOF, genomics, and cytokine analysis, helping to improve cancer treatments and patient outcomes.

NMR Core Facility is equipped with NMR spectrometers allowing researchers to Characterize bio-molecular structures at the atomic resolution with exquisite details;Identify protein interactions with binding partners in order to understand their architecture;Study protein dynamic;Study protein post-translational modifications acting as molecular switches;Study structure-function relationships of biological proteins to gain insight into biological processes in relation to human disease;Screening of High-throughput drug-like leads for novel therapeutic targets;Study Metabolites of interest.Core facility houses high-field NMR spectrometers equipped with cryogenically cooled probes (1H or 13C optimized) for enhanced sensitivity.

Core offers services in optical microscopy experiments including: confocal, multiphoton, super-resolution, FRAP, FRET, live-cell timelapse, whole-slide scanning, image analysis and more.

Core provides biospecimen processing and performs automated slide-based biomarker methods (protein/RNA/DNA), implementing good clinical laboratory practices.Offers specialized consultation and perform biomarker development and analyses within clinical study and research program protocols.Provides services to the clinical trials and pre-clinical research community and expertise in biomarker selection, method development, optimization and standardization, and provision of quality consultation on biomarkers for clinical research protocols.

Core offers services in Analytical Flow Cytometry, Fluorescence Activated Cell Sorting, consultation and data analysis. Staff provides training, assisted acquisition, consultation, and data analysis upon request.

Core provides services including Basic High-Throughput Sequencing Analysis, Advanced Data Analysis, High Performance Computing.

Offers services to support studies in medical fields across oncology, cardiology, metabolic diseases, radiotheranostics and more.Helps with study design to final data analysis, including GLP-compliant histopathology services.

Core provides services for the use of patient-Derived tumor Organoid (PDO) model.Organoid models are derived from patient-derived xenograft tissues, primary tumors, and metastatic patient tissues. PMLB organoid repository includes models generated from colorectal, pancreatic, and lung cancer tissues.

Core offers cell sorting and flow cytometry services. Provided instruments include BD FACS Canto II, Beckman Cytoflex LX, BD FACS ARIA III, AutoMacs Cell Separator.

Core offers services including Study planning and design; Grant writing and protocol creation; Data collection form design; Data capture instrument construction; Complex data processing and linkage; Quality control involvement; Statistical analysis, figure, and table generation; Manuscript drafting and presentation preparation; Response to manuscript reviews from regulatory agencies and journals.

Provides testing and analysis support for clinical trials to improve consistency and reliability in clinical trial data.Helps clinical investigators achieve systematic image collection and improved collaboration. Can help with handling large volume functional and dynamic imaging data.

Facility provides analytical flow cytometers and high-speed cell sorting, as well as training, education and consultation services.

Core is dedicated to providing access to cost-effective and high quality genomics services including standard and custom-tailored DNA sequencing using Illumina technologies, epigenomics, panel-based profiling (Luminex and Nanostring) and functional genomics. Single cell genomics applications on variety of popular platforms.

Core provides services for the use of Xenograft (PDX) models listed in open global catalogue of PDX models for comprehensive search and attribute filtering support for clinico-pathological and molecular data. Molecular data and visualization of profiled models could be accessed through the institute's portal.

Core offers biobanking services and diverse inventory of biospecimens in the field of Oncology. Offers prospective protocol specific banking tailored to your research needs, including patient eligibility, screening, consenting, biospecimen procurement, handling, and processing, clinical data annotation, short and long-term sample storage, and shipping.

Core supports biomarker discovery and enhancing future cancer research in the areas of genomics, proteomics and epidemiology. Provides serialized collections of biospeciments throughout patient prognosis. Clinical time point collections are based on input from our expert surgical, medical, and radiation oncologist teams.

Core services include Imaging Modalities:MRI,PET-MRI,CT,PET-CT,Ultrasound / X-Ray,Interventional / Biopsies;Consultation:Pre-grant submission consultations,Pre-trial launch consultations / site initiation visists,Feasibility assessments MRI physics consultation; Protocol Development / Site Feasibility:Industry site feasibility questionnaires, Protocol development of medical imaging studies and clinical trials; Post-Processing of Images:Tumour measurements, Volumetric measurements.

Core provide services for the enhancement and progression of novel and innovative research and discovery. Provides human biospecimen samples such as: fresh frozen and formalin fixed paraffin embedded (FFPE) tissue, compartmentalized blood components such as serum, plasma, and whole blood peripheral blood mononuclear cells (PBMCs) and other biofluids, including saliva and urine; Tissue microarray (TMA) construction and quantitative image analysis of TMA sections, Laser capture microdissection (LCM), digital slide scanning, quantitative image, and pathological/clinical analysis for various tumour sites and DNA/RNA extraction; support and guidance to researchers and biobanks on accessing, collecting, and using human biospecimens and associated data for health research.

The Advanced Optical Microscopy Facility (AOMF) is centrally located in the Discovery District in Toronto, Ontario, serving University Health Network as well as external academic and industrial users. It is the largest microscopy facility in Canada with instruments at Princess Margaret Hospital and Toronto General Hospital. With over 30 instruments and 4 staff members, the AOMF is equipped to help you with nearly any optical microscopy application including: confocal, multiphoton, FRAP, FRET, live-cell, timelapse, spectral unmixing, in-vivo bioluminescence and fluorescence, whole-slide scanning, 3D rendering, colocalization and more.

We offer:
- Free consultations to determine which microscopes would best suit your application
- Comprehensive training and support on our various instruments
- Full-service image capture and analysis (a great way to get started quickly)
- High-resolution whole slide scanning service for histology slides
- Practical courses on microscopy, including one-on-one hands-on sessions (eg: Fundamentals of Microscopy) and larger workshops tailored to your group's applications
- Software and training for image processing and quantification

The proteomics facility of the Network Biology Collaborative Centre (NBCC) provides mass-spectrometry based identification and quantification of proteins and is open to all users. Directed by Dr. Anne-Claude Gingras, the facility specializes in functional proteomics with a focus on protein proximity and protein interaction mapping.

Our full-service offerings include access to reagents (plasmids), sample preparation, mass spectrometry, and data analysis.

Applications include:
proximity-dependent biotinylation mapping to identify neighboring proteins in cells
protein-protein interaction mapping
differential protein abundance
post-translational modification identification (with a focus on phosphorylation)
chemical proteomics (identifying on- and off-target binding to small molecules or biologics)

The high-throughput screening facility of the Network Biology Collaborative Centre (NBCC) provides cutting-edge instrumentation and in-depth expertise for the automation of laboratory processes. Directed by Dr. Jeff Wrana, the facility has 2 automated screening platforms and accessory equipment to enable flexible and scalable in vitro and in vivo assays (including organoids).

Our full-service offerings include access to chemical and genome-wide siRNA libraries, assay development and implementation, and data analysis.

Applications include:
drug discovery and lead validation
ELISA-based assays for immune surveillance
protein biomarker discovery using OLINK proteomics technology
multiplexed barcode screening assays

The next-generation sequencing facility of the Network Biology Collaborative Centre (NBCC) provides both short and long read sequencing capabilities for the analysis of gene function and expression. Directed by Dr. Jeff Wrana, this facility specializes in functional genomics and single cell biology.

Our full-service offerings include consultation, sample preparation (especially for spatial and single cell applications), quality control, sequencing analysis, bioinformatics support, and assistance with grant and publication writing related to the services we offer.

Applications include:
- gene function analysis - pooled CRISPR screening, multiplexed barcode screens
- whole genome sequencing - both short- and long-read sequencing
- bulk gene expression profiling
- single cell biology (single cells and single nuclei)
- spatial profiling
- pathogen detection and profiling

The advanced imaging facility of the Network Biology Collaborative Centre (NBCC) covers all microscopy needs from your everyday workhorse to super-resolution. Directed by Laurence Pelletier, the facility is affiliated with the Nikon Centre of Excellence at the Lunenfeld-Tanenbaum Research Institute where users have access to the newest technologies in advanced microscopy.

Our full-service offerings include consultation on experimental design, advice on appropriate systems, training and support for assisted and unassisted use, as well as help with data analysis and image generation for publications.

Applications:
Fluorescence and brightfield image acquisition
Whole-slide scanning
Dynamic live-cell imaging
Super-resolution imaging

The flow cytometry facility of the Network Biology Collaborative Centre (NBCC) provides an extensive variety of state-of-the-art flow cytometry analyzers and cell sorters, with a specialty in image-based flow cytometry and cell sorting.

Our full-service offerings include access to a full suite of analyzers and cell sorters, unassisted and full-service cell sorting. comprehensive training on theory and on instruments, consultation on experiment design, protocol development, data analysis, manuscript preparation and grant submission.

Applications include:
- image-based analysis powered by machine learning on the Amnis ImageStream
- image-based cell sorting using the BD BACS Discover S8
- cell sorting for a variety of applications including multi-color sort for bulk culture, indexed cell sort into 96-well plates, cell cycle phase sort and primary tissue sorting for RNA or DNA analysis

The high-content screening facility of the Network Biology Collaborative Centre provides fast, reliable and high-content optical imaging and analysis of fixed and live cells: more data, more measurements for quicker discoveries.

Our full-service offerings include assistance with selecting the appropriate imaging platform, developing customized imaging assays, training in imaging and data analysis and assistance with image/data preparation for presentations and publications.

Applications:
Multi-plate based imaging applications including:
High-resolution cell characterization and sub-cellular localization using IN cell Analyzers
3D Spheroid and Organoid imaging using a Nikon A1R BioPipeline
Live-cell analysis (confluency, growth tracking) using an Incucyte - imaging within an incubator

Core offers services including cell sorting,cell analysis, user training for the flow cytometer benchtop analyzers, user training for the widefield and confocal microscopes.