The CoreMarketplace: Network Biology Collaborative Centre Proteomics

The proteomics facility of the Network Biology Collaborative Centre (NBCC) provides mass-spectrometry based identification and quantification of proteins and is open to all users. Directed by Dr. Anne-Claude Gingras, the facility specializes in functional proteomics with a focus on protein proximity and protein interaction mapping.

Our full-service offerings include access to reagents (plasmids), sample preparation, mass spectrometry, and data analysis.

Applications include:
proximity-dependent biotinylation mapping to identify neighboring proteins in cells
protein-protein interaction mapping
differential protein abundance
post-translational modification identification (with a focus on phosphorylation)
chemical proteomics (identifying on- and off-target binding to small molecules or biologics)

Facility Policies

Services are offerred outside of Lunenfeld-Tanenbaum Research Institute

Consulting is offerred outside of Lunenfeld-Tanenbaum Research Institute

(To see more facility information, click the in the upper right)

Services offered:

Assay Development

Mass Spectrometry - Proteins And Peptides

Proteomics

Proteomics Analysis

Proximity-Dependent Biotinylation

Quantitative Phosphoproteomics

Quantitative Proteomics - TMT

Sample Preparation

Facility Equipment

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer

Spectrometer performs analysis including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, and MSn analysis of small molecules. System incorporates the brightest ion source, segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology, and ETD HD. [Product Link]

RRID:SCR_020562

cite this instrument

Bruker timsTOF Pro 2

Bruker’s timsTOF Pro 2 is our workhorse for standard workflows such as protein-protein interaction and global proteome studies, enabling the identification of 6000 proteins at 30 samples per day.

Bruker timsTOF SCP

Bruker’s SCP is a single cell proteomics solution, enabling detection of 4000 proteins for a single-cell level sample (250pg) at 40 samples per day.

SCIEX TripleTOF® 6600 Mass Spectrometer System

Thermo Scientific Orbitrap Astral

The Orbitrap Astral excels when the highest degree of proteome coverage and sensitivity is required and enables the identification of 8000 proteins in a single global proteome sample at 80 samples per day.

No additional equipment has been listed