The mission of the University of Colorado Anschutz Medical Campus (CU AMC) Drug Discovery and Development Shared Resource (D3SR) is to provide expertise and infrastructure to facilitate drug discovery at different stages of the development pipeline, including assay development and validation, high-throughput screening (HTS) and image-based high-content screening (HCS) discovery, hit-to-lead validation and optimization, and in vitro and in vivo pharmacology including pharmacokinetics (PK) and pharmacodynamics (PD) as well as toxicology.

GSR provides genome-wide analyses of DNA sequence, transcriptomes, epigenetics and cytogenomics using next generation sequencing (NGS) and gene microarrays; and single cell genomic and transcriptomic analyses including single cell immune profiling and spatial gene expression.

The aim of the Cryo-EM SR is to provide state-of-the-art equipment, expert technical assistance and image analysis expertise to enable all aspects of macromolecular structure determination by single particle cryo-electron microscopy

The goal of the Biomolecular NMR Core facility is to enable the study of biomolecular structure, dynamics and interactions in solution at the atomic level. The facility provides is equippped with three high field NMR spectrometers from 600 MHz to 900 MHz equipped for biomolecular NMR studies

Service facility which provides instrumentation and image analysis on recharge basis. Offers full specimen preparation and imaging services. Specimen preparation for electron microscopy studies including negative staining, plastic embedding, ultramicrotome and immunogold.

The Genetically Engineered Murine Models (GEMM) Core provides full-service mouse genetic engineering support to researchers. We will help you select the best model for your research program, design and build any requisite constructs and reagents, and produce live mice. The most popular requests are for CRISPR-based gene edited mice and transgenic mice. To date, we have created engineered alleles for knockouts, conditional knockouts, SNP knockins, tag and marker insertions, deletions of up to 300kb, duplications, and transgenic alleles. These novel mouse models have been made on a wide spectrum of genetic background strains, including but not limited to C57BL/6J, C57BL/6N, NOD, BALB/cJ, FVB/Crl, NSGS, various 129 substrains, collaborative cross strains, and on mice that are already genetically modified.

Our robust breeding and genotyping program supports breeding to establish germline transmission and can also create cohorts for investigators. We also provide strain cryopreservation, rederivation and rescue services, as well as cryostorage. Our services are available to outside institutions, nationally and internationally.

The Population Health Shared Resource (PHSR) supports access to and expertise in data management, analyses, and mapping/data visualization of population-based databases. These databases are ideal for studies requiring access to large samples of patients and/or providers; examination of trends in treatment, screening, or other healthcare services; the impact of policy changes or recommendations on healthcare services; investigating the dissemination of evidence-based technologies and interventions; disparities of treatment or other health care services between groups; geographic distributions of cancer patients and relationships with other geospatial data.

Biostatistics provides quantitative support for the planning, design, analysis, and presentation of basic science, clinical, and epidemiological investigations. We are dedicated to delivering high-quality, methodically developed results to improve patient outcomes and clinical care.

The UCD AMC gnotobiotic core provides investigators germ free (axenic/sterile) and gnotobiotic (flora-defined) mice. We provide a space in which investigators can complete their own studies or hire the core to complete experimental manipulations. Our core facility includes 12 large soft-sided vinyl isolators for breeding and 8 small soft-sided vinyl isolators for experiments. We also have the option between using the Allentown positive pressure racks and static cage for experimental use.

The Small-Animal IGRT Core specializes in the targeted irradiation of small animals for use in pre-clinical trials of radiation therapy. Through the use of the XRAD-SmART Irradiator, our core can use x-ray fluoroscopy and on-board CBCT to accurately irradiate small lesion areas while protecting normal tissue. Our core also provides experiment design, data analysis, and dosimetry calculations, as well as longitudinal acquisition of CBCT for temporal assessment of tumor growth kinetics.

At HIMSR we facilitate clinical and translational studies by dissecting immune-based mechanisms with diagnostic and therapeutic applications. The assays we offer include immunophenotyping and functional characterization, multicolor fluorescence tissue imaging, multiplex cytokine analysis, and cellular assays

The goal of the Biological Mass Spectrometry Facility is to provide investigators with the capabilities to identify, characterize, and quantify biomolecules present in tissues, cells, and biological fluids. The facility houses both Proteomics and Metabolomics Cores, and aims to assist members with solving difficult or previously intractable problems in biomedical research. Methods for biomolecule isolation, separation, quantification, identification and bioinformatics analysis, together with expert guidance in study design, are integrated into expertise offered by the Facility.

The Organoid and Tissue Modeling Shared Resource facilitates access, generation, and usage of novel in vitro mouse and human organoid systems to promote innovative basic and translational research and disease modeling. The OTMSR also provides expertise in genome editing technologies for targeted knock-outs, point mutations, and reporter generation in organoid systems. The OTMSR additionally provides many specialized medias and reagents to support most common organoid culture systems

The main service of THE PHISM core is to provide Human Immune System (HIS) or “humanized” mice as a pre-clinical in vivo model to study the human immune system in an experimental tractable animal model (e.g. immune response to human tumors that readily grow in HIS-mice). In addition to providing the HIS-mice to investigators, we also offer immune expertise, experimental procedures and data analysis of immune-based assays, such as flow cytometry.

The major goal of the Animal Imaging SR is to enable non-invasive imaging of animal models of human disease. Our Core facilities provide access and training on state-of-the-art animal scanners (MRI, CT, optical, PET/SPECT) for live imaging on rodents, perfoms all animal handling and assist with image analysis to establish imaging biomarkers

The Pathology Shared Resource Biorepository Section provides a wide array of services to support biobanking, clinical trials, and research studies. We provide access to high-quality non-biased patient specimens collected from clinical and research procedures that are handled, processed, analyzed, and interpreted in a uniform manner. Services include, collection, processing, storage, clinicopathological annotation, and distribution of biospecimens

The Pathology Shared Resource, in collaboration with Colorado Genetics Lab, provides cytogenetic and cytogenomic research services. Services include tissue culture, chromosome analysis, fluorescence in situ hybridization (FISH), and chromosomal microarray. We also offer the design and development of customized FISH probes. We are available for consultation on study design and data analysis. 

The Pathology Shared Resource Research Histology Section provides a wide range of standardized paraffin histology procedures that can be tailored to specific research needs.  Services include tissue grossing/cassetting, paraffin processing, sectioning, routine and special stains. We specialize in immunohistochemistry and antibody optimization procedures.

Our goal is to promote scientific research in Colorado through the use of Functional Genomics. Our ultimate goal is to catalyze discoveries that will positively impact the quality of human life.

We achieve this by: 1) Facilitating access to tools (shRNA, ORF, CRISPR) to investigate gene function on a genome-wide scale. 2) Providing protocols and expertise for the use of these genomics tools. 3) Serving as a forum for scientific exchange and discussion in the field of functional genomics.

The Cell Technologies Shared Resource (CTSR) supports basic and translational research projects in biomedical research. CTSR produces hybridomas/monoclonal antibodies to enhance basic research and preclinal studies and makes recombinant proteins for mechanistic and structural studies on proteins. We provide multiple platforms and analytic modules for real time live-cell imaging of cultured cells and organoids to enhance analysis of cancer cell biology. In addition, we maintain a collection of over 500 authenticated human cell lines for use in biomedical research.

The Xray Crystallography Core performs all aspects of structure determination of karge biomolecules (such as proteins and nucleic acids) including macromolecular crystallization, crustal screening, molecular structure and model building

We are a cancer-focused bioinformatics group that assists in all needs related to -omics and big data. We assist our collaborators with experimental design, data processing, interpretation, generating publication-quality figures, and preparing manuscripts and grants.