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University of California, Santa Cruz
1156 High St. (PSB 290)
Santa Cruz, CA 95060United States of Americahttps://strucbiol.pbsci.ucsc.edu/
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Last Updated: 06/01/2021
The Macromolecular Structure Function Core Facility (MSFCF) supports Biochemistry and Structural Biology researchers at UC Santa Cruz and the surrounding area. We provide state of the art instrumentation, supplies, and expertise to assist researchers in the structural characterization of biological macromolecules. Resources are available for crystallization and structure determination as wells as biophysical characterization of macromolecular stability and interactions.
Services are offerred outside of University of California, Santa Cruz
Consulting is offerred outside of University of California, Santa Cruz
Art Robbins Crystal Gryphon Liquid Crystal Polymer Setup System
Gryphon LCP is a setup system for Lipidic Cubic Phase sandwich plates and bicelle assays as well as traditional protein crystallography plates. A dedicated syringe based LCP head dispenses the protein, and a 96 head dispenses the buffer solutions. A 96 well sandwich plate can be sealed in less than 2 minutes. An optional non-contact dispenser can be added at any time to set up vapor diffusion sitting drop and hanging drop plates. The Crystal Gryphon is a small, fast and option for setting up all of your crystal plates. The Crystal Gryphon fits into tight lab spaces and tight lab budgets. The 96-channel positive displacement head quickly fills screen reservoirs and protein wells. The head has no disposable tips, so the cost of operation and environmental waste is minimized. The 1-channel non-contact dispenser quickly aspirates and dispenses your proteins allowing your plate to be sealed without evaporation being a problem. This dispense head is designed specifically to handle proteins, features flow-through washing and positive displacement aspiration, to handle all of your proteins. [Product Link]
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General Electric AKTA FPLC System, Protein Purification System
Purification system intended for microgram-scale protein purifications in Tricorn columns up to milligram-scale using HiTrap and HiScreen columns. For fast protein liquid chromatography of tens of grams of protein,can be used with flow rates up to 150 mL/min. This high flow rate is necessary when working with high-flow resins in large columns. Columns can even be packed at flow rates as high as 300 mL/min. System reduces hands-on time so that you can spend less time purifying proteins and more time focusing on your results. Easily switch between different users and applications. To get you up and running quickly, methods are easily created by dragging and dropping predefined templates in UNICORN 7. Purification of protein to sufficiently high yield and purity can be a time- and resource-consuming process. To address fast and efficient protein purification, Can be set up in different ways to handle multistep purification. With an additional column valve up to 10 columns can be connected enabling automation of several samples and steps. [Product Link]
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Microcal VPViewerTM Differential Scanning Calorimeter VP--DSC
Differential scanning calorimeter for study of samples in solution. It measures intramolecular stability of proteins, nucleic acids, oligomeric proteins, nucleic acid duplexes, lipid and detergent micellar system. [Product Link]
Citation IDs: NIH S10OD028725
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No additional equipment has been listed
Publications associated with this facility (Click To View):
1.) Schwechheimer C, Hebert K, Tripathi S, Singh PK, Floyd KA, Brown ER, Porcella ME, Osorio J, Kiblen JTM, Pagliai FA, Drescher K, Rubin SM, Yildiz FH (2020 Aug). A tyrosine phosphoregulatory system controls exopolysaccharide biosynthesis and biofilm formation in Vibrio cholerae. PLoS pathogens, 16(8), e1008745. doi: 10.1371/journal.ppat.1008745. ID: 32841296.
2.) Vorster PJ, Goetsch P, Wijeratne TU, Guiley KZ, Andrejka L, Tripathi S, Larson BJ, Rubin SM, Strome S, Lipsick JS (2020 May 7). A long lost key opens an ancient lock: <i>Drosophila</i> Myb causes a synthetic multivulval phenotype in nematodes. Biology open, 9(5), e1008745. doi: 10.1371/journal.ppat.1008745. ID: 32295830.
3.) Fribourgh JL, Srivastava A, Sandate CR, Michael AK, Hsu PL, Rakers C, Nguyen LT, Torgrimson MR, Parico GCG, Tripathi S, Zheng N, Lander GC, Hirota T, Tama F, Partch CL (2020 Feb 26). Dynamics at the serine loop underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing. eLife, 9(5), e1008745. pii: e55275. doi: 10.7554/eLife.55275. ID: 32101164.
4.) Philpott JM, Narasimamurthy R, Ricci CG, Freeberg AM, Hunt SR, Yee LE, Pelofsky RS, Tripathi S, Virshup DM, Partch CL (2020 Feb 11). Casein kinase 1 dynamics underlie substrate selectivity and the PER2 circadian phosphoswitch. eLife, 9(5), e1008745. pii: e52343. doi: 10.7554/eLife.52343. ID: 32043967.
5.) Vorster PJ, Goetsch P, Wijeratne TU, Guiley KZ, Andrejka L, Tripathi S, Larson BJ, Rubin SM, Strome S, Lipsick JS (2020 Jan 1). A long lost key opens an ancient lock: Drosophila Myb causes a synthetic multivulval phenotype in nematodes. Biology open, (), . . ID: 34004684.
6.) McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG (2019 Dec 17). Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection. Proceedings of the National Academy of Sciences of the United States of America, 116(51), 25602-25613. pii: e52343. doi: 10.7554/eLife.52343. ID: 31796585.
7.) Guiley KZ, Stevenson JW, Lou K, Barkovich KJ, Kumarasamy V, Wijeratne TU, Bunch KL, Tripathi S, Knudsen ES, Witkiewicz AK, Shokat KM, Rubin SM (2019 Dec 13). p27 allosterically activates cyclin-dependent kinase 4 and antagonizes palbociclib inhibition. Science (New York, N.Y.), 366(6471), e1008745. pii: e52343. doi: 10.7554/eLife.52343. ID: 31831640.
8.) Guiley KZ, Iness AN, Saini S, Tripathi S, Lipsick JS, Litovchick L, Rubin SM (2018 Oct 2). Structural mechanism of Myb-MuvB assembly. Proceedings of the National Academy of Sciences of the United States of America, 115(40), 10016-10021. doi: 10.1021/jacs.8b13079. ID: 30224471.
9.) Toor JS, Rao AA, McShan AC, Yarmarkovich M, Nerli S, Yamaguchi K, Madejska AA, Nguyen S, Tripathi S, Maris JM, Salama SR, Haussler D, Sgourakis NG (2018). A Recurrent Mutation in Anaplastic Lymphoma Kinase with Distinct Neoepitope Conformations. Frontiers in immunology, 9(21), 99. doi: 10.3389/fimmu.2018.00099. ID: 29441070.
10.) McGrath DA, Fifield BA, Marceau AH, Tripathi S, Porter LA, Rubin SM (2017 Aug 1). Structural basis of divergent cyclin-dependent kinase activation by Spy1/RINGO proteins. The EMBO journal, 36(15), 2251-2262. doi: 10.3389/fimmu.2018.00099. ID: 28666995.
11.) Liban TJ, Medina EM, Tripathi S, Sengupta S, Henry RW, Buchler NE, Rubin SM (2017 May 9). Conservation and divergence of C-terminal domain structure in the retinoblastoma protein family. Proceedings of the National Academy of Sciences of the United States of America, 114(19), 4942-4947. doi: 10.1039/c7mb00163k. ID: 28439018.
12.) Tseng R, Goularte NF, Chavan A, Luu J, Cohen SE, Chang YG, Heisler J, Li S, Michael AK, Tripathi S, Golden SS, LiWang A, Partch CL (2017 Mar 17). Structural basis of the day-night transition in a bacterial circadian clock. Science (New York, N.Y.), 355(6330), 1174-1180. doi: 10.1039/c7mb00163k. ID: 28302851.
13.) Michael AK, Fribourgh JL, Chelliah Y, Sandate CR, Hura GL, Schneidman-Duhovny D, Tripathi SM, Takahashi JS, Partch CL (2017 Feb 14). Formation of a repressive complex in the mammalian circadian clock is mediated by the secondary pocket of CRY1. Proceedings of the National Academy of Sciences of the United States of America, 114(7), 1560-1565. doi: 10.1039/c7mb00163k. ID: 28143926.
14.) Marceau AH, Felthousen JG, Goetsch PD, Iness AN, Lee HW, Tripathi SM, Strome S, Litovchick L, Rubin SM (2016 Jul 28). Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters. Nature communications, 7(7), 12301. doi: 10.1039/c7mb00163k. ID: 27465258.
15.) York RL, Yousefi PA, Bogdanoff W, Haile S, Tripathi S, DuBois RM (2015 Dec 9). Structural, Mechanistic, and Antigenic Characterization of the Human Astrovirus Capsid. Journal of virology, 90(5), 2254-63. doi: 10.1039/c7mb00163k. ID: 26656707.
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