High-end microscopy imaging capabilities, including bright field (color, DIC), fluorescence (multi-dimension; TIRFM; bleaching, ablation, FLIM), high-resolution, highspeed, high-sensitivity, confocal, and physiologic techniques; STED, GSD3D, lightsheet selective plane illumination (SPIM), and lattice lightsheet structured illumination super resolution microscopy

The UChicago Sanger Sequencing Core provides Sanger sequencing and fragment analysis via capillary electrophoresis. Expected turnaround time is within 2 business days of order receipt.

Facility in the Department of the Geophysical Sciences features instruments including TESCAN LYRA3 field-emission scanning electron microscope (FESEM) with focused ion beam (FIB). It is equiped to do EDS, WDS, EBSD, etc.

The Single Cell Immunophenotyping Core Facility is a full-user self-service facility that provides access to specialized instrumentation for the phenotyping and genotyping of immune cells. These include single-cell next-generation sequencing and quality control technologies. All instruments are located in the laboratories of four different CIIC investigators (KCBD 10220E and 10220C), and are available for use by the larger UChicago community.

The Cytometry and Antibody Technology (CAT) Facility’s mission is to support the community of researchers at the University of Chicago with (1) cutting-edge technology operated by expertly trained personnel; and (2) expert technical consultation and training of researchers.

Core utilizes dual tube micro X-ray computed tomography scanner for imaging specimens in fields of paleontology, comparative anatomy, evolution and development, basic biomedical studies, and research in geology and material sciences.It is equipped with 180 kV nano-focus and high power 240 kV micro-focus CT tube. This setup allows to scan very small specimens with high resolution using nano tube or dense fossils using high power tube.

MRI Research Center houses magnetic resonance scanners and highly qualified experienced staff for support of research projects with MR imaging component.

The Cellular Screening Center (CSC) provides high throughput cell and non-cell based screening for researchers. Both small molecules and siRNA libraries are offered for such screens.

Our goal is to assist University of Chicago investigators and research staff with medical imaging exams performed on human subjects for research purposes. This includes the acquisition, analysis, collection, de-identification and distribution of image data for both basic science research and clinical trials. Our services ensure research-related imaging fulfills protocol requirements and allow investigators to obtain HIPAA- and IRB-compliant clinical research data. We provide our services for all types of medical imaging, including x-ray, CT, MRI, ultrasound, nuclear medicine, PET, and most types of specialty imaging.

The platform is equipped with the high-resolution Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer(+UVPD)- Vanquish Horizon UHPLC system. We have experience in quantifying diverse groups of metabolites in a wide variety of matrices such as cells, culture medium, bodily fluid, animal tissue, exhaled breath condensate, etc., and performing stable isotope tracing experiments using 13C, 15N, and 2H-labeled substrates. We support hypothesis-generating untargeted and hypothesis-driven targeted metabolomics studies from the experimental design stage through data acquisition, data analysis, and interpretation.

Next-Gen (Illumina and Pacific Biosciences Sequencing and Library prep), NovaSEQ-X-Plus, Micro-array (Illumina genotyping and EPIC arrays), Sanger DNA Sequencing, and Bioanalyzer/Fragment-analyzer sample QC services. For Single Cell sequencing project the Facility operates a DROP-SEQ and 10X Genomics instrument

The ETSG serves University of Chicago faculty-led research projects. ETSG staff is available to help plan, design, build, test, and install mechanical and electro-mechanical apparatus.

The Pritzker Nanofabrication Facility is an ISO Class 5 cleanroom that specializes in advanced lithographic processing of hard and soft materials.

The mission of the facility is to work in collaboration with groups from UChicago and external organizations or companies to develop and utilize top-notch proteomics techniques to answer both basic and translational scientific questions.

The mission of the Organoid and Primary Culture Research (OPCR) Core is to facilitate access, generation, banking, and usage of organoids and primary cells derived from patients and model organisms.

The goal of the Human Disease & Immune Discovery core facility is to provide expertise, equipment and physical space for researchers to perform cutting edge, high resolution investigations of human immune system. We leverage innovative technologies from field of flow cytometry for data generation and analysis and through close collaboration with Genomics core facility we can provide cellular component solutions to even higher resolution genomic investigations.

The facility houses a Bruker AVANCE IIIHD 600 and a Bruker AVANCE III 500 NMR spectrometer. These are equipped with high sensitivity inverse and broadband probes, single axis gradients, pulse shaping capabilities, variable temperature control(-50˚C to 150 ˚C), and automatic tuning and matching. The Bruker AVANCE IIIHD 600 is also equipped with a robotic sample changer (Bruker SamplExpress) allowing automated acquisition of up to 60 samples. Sample preparation can also be automated with our Gilson sample preparation robot.

The analysis of data that transforms high-throughput raw data into biologically meaningful information can present a challenge to clinical, translational, and basic researchers alike. The CRI Bioinformatics Core offers services and expertise designed to allow BSD investigators to take full advantage of high-throughput technologies.

Established in 2007, the GRAF is now one of the largest and most successful academic gnotobiotic mouse facilities internationally.  GRAF is a centrally managed gnotobiotic/germ-free mouse facility located within the centrally managed Knapp Center for Biomedical Discovery (KCBD) vivarium.  Both are a component of The University of Chicago’s AAALAC International accredited Animal Care and Use Program.  The GRAF currently maintains over 80 flexible film isolators with capacity for greater than a 1045 mouse cages. 

The Facility was established in 1989 and provides transgenic (plasmid and BAC) and CRISPR (also ROSA26 & H11 CRISPR) injections, ES Cell targeting, screening, culture and blastocyst injections, rederivation, breeding and mouse services and consultation on project development. The facility also now offers CRISPR vector design and development, as well as design and development of CRISPR guides.

The mission of the Animal Resources Center is to assist investigators in performing their animal research while ensuring appropriate and humane care of all laboratory animals.

Cleanroom (ISO7/8) environment for manufacturing and preparing human cells, tissues and tissue-based
products for treatment of patients for Phase I, II and III clinical trials. CAP Accredited

A coordinated, centralized, and dedicated program for the procuring, processing, dispersing and assessing all types of biospecimens together with downstream histology services. CAP Accredited

Titan Krios with Gatan K3 detector, Aquilos Cryo-DualBeam system, Apreo Volume Scope Serial Blockface SEM, FEI Talos Cryo-EM

Our goal is to provide University of Chicago investigators and external users with state of the art, quantitative multi-modality imaging technologies to advance in-vivo molecular and physiological research of a broad range of disease and cancer models, and to accelerate pre-clinical development of novel therapeutics and diagnostic tools.

The Human Immunologic Monitoring (HIM) facility serves as a specialized laboratory performing correlative assays for cancer-based clinical trials. We participate in project development to assist in assay selection and optimization prior to study initiation. We offer a pick-up service for specimens from patients at the U of C Hospital or outpatient clinics, and process the samples as indicated for the assays being performed. For some studies, isolation and freezing of peripheral blood lymphocytes for analysis at a later date is performed, while for others immediate staining of whole blood for specific cell surface markers of interest is carried out. In addition, our laboratory prepares clinical grade products, such as peptide vaccines, for administration into patients.

We train labs to use the latest episomal techniques to reprogram, expand and characterize human and mice iPS cells from skin or blood tissues of healthy subjects and diseased patients. We also develop the capability to differentiate the iPS cells into specific somatic cells, such as neutrons, cardiomyocytes, and hepatocytes.

In the era of genomics-driven biology, one must be able to study not only the sequences of genes, but the structures and functions of the molecules they encode, both the proteins and functional RNAs. Characterizing the structure and dynamics of these molecules is crucial to understanding the basis of nearly every cellular function.