In Vivo Preclinical Imaging

The Mutant Mouse Resource and Research Centers (MMRRC) function as a dynamic network of research hubs, offering comprehensive resources and assistance for scientists employing mice in their research. Established by the National Institutes of Health (NIH), the MMRRC‘s primary mission is to serve as a centralized hub for the production, distribution, and detailed analysis of mutant mouse strains. Across various locations in the United States, the MMRRC presents an array of services that bolster research, including:

Advanced Mouse Colony Management: The MMRRC manages an extensive mouse colony for research applications. They provide sophisticated breeding, genotyping, and cryopreservation services, crucial for creating and sustaining mice with specific genetic traits or alterations.

Cutting-edge Molecular Biology and Genomics: The centers have advanced molecular biology and genomics research facilities. These include high-throughput DNA sequencing, microarray analysis, and comprehensive gene expression profiling.

Exemplary Animal Care and Ethical Standards: The MMRRC‘s facilities are designed to surpass regulatory benchmarks for ethical and humane animal care in research settings. They feature specialized environments for housing, handling, and nurturing the mice, supported by a team of professionals trained in optimal animal care and welfare.

Comprehensive Training and Educational Programs: The MMRRC actively contributes to the growth of students and researchers in mouse biology through various educational initiatives. These encompass hands-on workshops, seminars, and courses on mouse genetics, breeding strategies, and research methodologies.

In essence, the Mutant Mouse Resource and Research Centers are indispensable pillars for the scientific community, facilitating research utilizing mice to explore a broad spectrum of biological inquiries.

Core provides LC-MS/MS analysis including Protein ID, Proteomics Profiling, Targeted Proteomics and Post Translational Modification analysis. Analytical proteomic services are provided with emphasis on label free quantitative proteomic profiling, analysis of macromolecular complexes, post-translational modification of their constituents and standard protein identification from complex protein mixtures.

Real-time PCR Research and Diagnostics Core Facility accepts research projects from both academic and private sector. The Core Facility can accommodate projects involving laser microdissection, RNA and DNA extractions through semi-automated nucleic acid extractions systems, cDNA synthesis and complete Real-time analysis using a laser 7900 HTA FAST platform. Real-time PCR Research and Diagnostics Core Facility provides research results efficiently and rapidly while adhering to the highest quality standards and offers a unique opportunity to concentrate the latest equipment and knowledge in one location.

The Mouse Biology Program (MBP) at the University of California, Davis, is a research center that focuses on using mice as a model organisms to study a wide range of biological processes, including genetics, development, behavior, and disease. The MBP provides a range of resources and facilities to support this research, including:

1. Mouse colony management: The MBP maintains a large colony of mice used for research purposes. This includes breeding, genotyping, and cryopreservation services to support the production and maintenance of mice with specific genetic backgrounds or modifications.

2. Molecular biology and genomics: The MBP has a range of facilities and resources for molecular biology and genomics research, including DNA sequencing, microarray analysis, and gene expression studies.

3. Animal care and use: The MBP has a state-of-the-art animal care facility that meets or exceeds all regulatory standards for humane animal care and use in research. This includes specialized housing, handling, and husbandry facilities, as well as trained staff to support the care and welfare of mice used in studies.

4. Training and education: The MBP provides a range of training and educational opportunities to support the development of students and researchers in the field of mouse biology. This includes workshops, seminars, and courses on mouse genetics, breeding, and research techniques.

Overall, the Mouse Biology Program at UC Davis is a crucial resource for researchers and students interested in using mice as model organisms to study a wide range of biological processes.

UC Davis’ Institute for Regenerative Cures offers leading-edge research services for Investigational New Drug (IND) development. Located in Sacramento and anchored by Northern California’s largest academic Good Manufacturing Practice facility, the institute’s fee-for-service cores and support services include pre-clinical transplantation assays in immune deficient mouse models, as well as an ability to scale-up manufacturing processes for novel cell and gene therapies. From bench to bedside, UC Davis can meet research needs with affordable services for investigators, including external academic and industry partners.

The BAE Shop provides support to UC research through the engineering, design, fabrication, and repair of custom equipment. We can take your ideas, napkin sketches, drawings, and models and turn them into real parts, machines, tools, and systems. Services: prototyping, welding, fabrication, machining, electrical, repairs, misc. technical labor.

Liquid and Solid State Nuclear Magnetic Resonance Spectroscopy at the University of California, Davis

Accredited by the College of American Pathologists, the Biorepository Shared Resource, or BRSR, provides well-characterized, high-quality, de-identified specimens with annotated data for clinical and basic science research purposes. The biorepository functions as a centralized tissue bank to provide researchers access to cancer- and non-cancer-related specimens (fresh/
frozen tissue, paraffin blocks/sections and fluids), procured and stored using international standards of best practices and protocols compliant with the Office for Human Research Protection. Specimens can be obtained prospectively as part of clinical trials or accessed through UC Davis Health Clinical Laboratories, where over 5.5 million blood specimens are processed annually.

Biorepository Shared Resource is part of UC Davis Health Comprehensive Cancer Center.

The In vivo Translational Imaging Shared Resource, or IVTISR, provides access to a broad range of in vivo imaging technologies including molecular imaging, optical imaging, quantitative physiologic and anatomic imaging, and whole-body PET/CT scanning in humans and animals. It also provides targeted imaging probes and tracers as well as expertise in planning, executing and analyzing in vivo imaging studies. The resource can support imaging studies in small animals, large animals and humans.

This resource is located in three adjacent buildings in the Health Sciences district of the Davis campus:

1 - Center for Molecular and Genomic Imaging (CMGI), Genome and Biomedical Sciences Facility (small-animal imaging)

2 - Center for Imaging Sciences (CIS), Veterinary School (large-animal imaging)

3 - EXPLORER Molecular Imaging Center; School of Medicine (human imaging)

In vivo Translational Imaging Shared Resource is part of UC Davis Health Comprehensive Cancer Center.

UC Davis Bioinformatics Core helps researchers with their projects, from experimental design, through all analyses and to publication. Facility has over a decade of experience in helping to solve a wide variety of bioinformatics problems, large and small. We have significant expertise in analyzing data from omics technologies, and other projects, to help drive research forward. Facility also offers bioinformatics training workshops regularly.

The UC Davis Flow Cytometry Shared Resource operates cell sorting and analytic cytometry equipment on the main academic campus in Davis, CA and in a separate facility at the UC Davis Medical Center in Sacramento, CA. We offer multiparameter cell sorting and analytic cytometry services, instrumentation training and comprehensive flow cytometry training for UCD, UC, and industry scientists. Comprehensive, week long flow cytometry training is offered each July, with lecture and laboratory instruction - this course is open to all interested participants and typically costs less than $1,000.00 for a full week of training. Single cell sorting and with phenotypic indexing is offered, as well as single cell targeted gene transcription analysis and genotyping on the Fluidigm BioMark platform. Additional cytometry services include tissue and plate scanning for cytometry of tissue samples and adherent cultures on the Compucyte Laser Scanning Cytometer. Please contact us for more information on these services.

Provides electron microscopy imaging services, expertise, training, training in sample preparation and equipment use.

The Genomics Shared Resource, or GSR, provides expertise and comprehensive services to accomplish virtually every application of next- generation sequencing-based genomics research, including gene expression profiling, mutation/variant analyses, copy number analysis, epigenomics, metagenomics, single-cell sequencing and spatial transcriptomics services. The GSR also specializes in the development of custom protocols and extensive data analysis and integrative bioinformatics support. The shared resource provides a wide range of additional services for translational and clinical genomics research.

Genomics Shared Resource is part of UC Davis Health Comprehensive Cancer Center.

The Biostatistics Shared Resource, or BSR, provides expertise in the design, analysis and reporting of cancer-related studies, including basic, translational, clinical and population-based research. BSR-affiliated faculty and staff work with investigators from the earliest stages of study planning. The shared resource is especially committed to mentoring early-career cancer researchers.

Biostatistics Shared Resource is part of UC Davis Health Comprehensive Cancer Center.

The Combinatorial Chemistry and Chemical Biology Shared Resource, or CCCBSR, provides a high throughput screening platform for users to discover unique chemical probes against biological targets using various one-bead one-compound and one-bead two-compound combinatorial libraries. The CCCBSR interacts closely with resource users on optimization of the lead compounds via focused libraries and standard medicinal chemistry techniques. In addition, the CCCBSR provides custom synthesis of telodendrimer-based micellar nanoparticle platform for efficient drug delivery.

Combinatorial Chemistry and Chemical Biology Shared Resource is part of UC Davis Health Comprehensive Cancer Center.

The Immune Monitoring, Analysis and Diagnostics Shared Resource, or IMADSR, is a CLIA-certified laboratory established to provide scientific support to investigators conducting clinical studies in humans, preclinical studies in animals and/or studies in veterinary medicine. It is well suited to conduct the immunology-based assays needed to monitor patients in clinical immunotherapy trials, but the IMADSR also offers a variety of different molecular and cellular assays for investigators in different fields, including custom assays designed to meet specific needs. Investigators at any of the University of California campuses, as well as outside investigators in industry and academia, are encouraged to contact the shared resource for research support.

Immune Monitoring, Analysis and Diagnostics Shared Resource is part of UC Davis Health Comprehensive Cancer Center.

The Molecular Pharmacology Shared Resource, or MPSR, provides services to support the development and implementation of clinical trials at the UC Davis Comprehensive Cancer Center. The MPSR oversees high-quality collection, processing, and analysis of clinical specimens (typically but not exclusively blood specimens) for pharmacokinetic and pharmacodynamics studies. The MPSR also conducts preclinical modeling of novel anti-cancer agents to test hypotheses and develop the scientific rationale required for translation of laboratory concepts into clinical trials, including the assessment of DM/PK/PD properties.

Molecular Pharmacology Shared Resource is part of UC Davis Health Comprehensive Cancer Center.

HSDAIF is a fluorescence microscopy facility with two Leica confocal microscopes, one with superresolution capability, the second with multi-photon imaging capability, and a Zeiss Lightsheet microscope. The facility offers consultation, training, and support.

Mass spectrometry core offering advanced mass spectrometry services. Serviced include analysis of intact molecules including peptides, proteins, oligonucleotides, polymers and small organic molecules. MALDI, LCMS or direct loop injections. High-resolution accurate mass (HRAM) measurements and tandem MS/MS available. Routine quantitation also available.

Our metabolomics core facility provides state-of-the-art high throughput services for the analysis of small molecules in biological samples. We specialize in the discovery, identification and quantitation of many metabolites and lipids. Our trained technical staff and 17 mass spectrometers ensure accurate and reliable results for the UC Davis community and beyond. Our goal is to support our clients in their research efforts by providing the most advanced metabolomics analysis services available, and help moving the field forward where it falls short. We also provide professional courses in metabolomics analysis and data processing.

The Advanced Materials Characterization and Testing laboratory (AMCaT) is a shared resource facility that is available to all interested users on and off campus. AMCaT supports a wide variety of electron microscopes, including aberration-corrected scanning transmission electron microscopes for the characterization of both hard and soft materials. These capabilities are complemented by state-of-the-art sample preparation equipment, high-resolution x-ray diffractometer, and a variety of different optical microscopes and image analysis software. AMCaT embraces and supports a multi-disciplinary user base of undergraduate, graduate, postdoctoral and faculty researchers at UC Davis and plays a key role in numerous research projects and lab classes.

The UC Davis Proteomics Facility offers protein analysis via amino acid analysis, Edman sequencing and LC-MS/MS including protein identification, post-translational modification discovery, label free quantitation and targeted proteomics. The facility utilizes state of the art instrumentation, including two Thermo Scientific Q-Exactive Mass spectrometers. Our group utilizes open source software and uses techniques and protocols that are made publicly available. Using open source software allows users to analyze their own data without significant monetary investment. We also offer data analysis and sample preparation classes to UC Davis and the community at large, including a week long hands on summer short course (see http://goo.gl/iScMe ). Our group operates as an open core facility with the Genome Center on the UC Davis campus

Genotyping on all the Illumina platforms, sequencing on Illumina GAIIx, HiSeq2000, MiSeq,and PacBio RS, array services for NimbleGen and Illumina, chromatin immunoprecipitation and methylated DNA immunoprecipitation services.