Facility Details

NEXT-GENERATION GENOMICS SERVICES

NGS Instruments: Currently we have two NGS systems, which generate both long and short sequence reads.

a) Illumina - HiSeq 1000

The Illumina system uses Sequencing by Synthesis (SBS) technology to sequence DNA fragments. HiSeq1000 generates over a billion reads of 100 bases per week. This system is ideally suitable for re-sequencing of genomes, transcriptome analysis, SNP discovery and structural variation analysis, DNA-protein interaction analysis (ChIP-Seq), sequencing-based methylation analysis, small RNA, etc.

- Whole Genome Sequencing
- Exome Capture & Sequencing
- Transcriptome Sequencing
- Small RNA Sequencing
- ChIP Sequencing
- Metagenome & Metatranscriptome sequencing

b) Roche 454 - GS Titanium FLX / GS FLX +

The Roche 454 Sequencer uses Pyrosequencing technology to sequence DNA fragments. The FLX + system is capable of generating greater than 1 million reads of 500-700 bases in 10 hours. The system is ideally suitable for de novo sequencing of whole genomes, transcriptomes, and metagenomic characterization of complex samples.

- de novo Whole Genome Sequencing
- de novo Transcriptome Sequencing
- Metagenome & Metatranscriptome sequencing
- Targeted resequencing