University of South Carolina at Columbia
Dept. of Pharmaceutical and Biomedical Sciences, University of South Carolina
715 Sumter Street, CLS 617
Columbia, SC 29208United Stateshttp://www.sccp.sc.edu/research/mcf/index.aspx
Dr. Kim E Creek
Last Updated: 11/29/2010
Services Provided: The USC Microarray Core Facility (formerly the SCCC DNA Microarray Facility) can produce Custom Arrays, support Array Design, conduct RNA quality control, amplification and labeling; conduct array hybridization, scanning and data analysis, and assist customers in all aspects of the design and performance of microarray experiments, using Agilent (pre-made and custom) and spotted arrays. In addition, the facility provides support for quantitation and quality assessment of DNA, RNA and protein on an Agilent 2100 Bioanalyzer.
The facility is equipped to provide custom microarrays for academic and industrial clients. So far we have successfully spotted cDNAs, 50 to 70 base pair oligonucleotides and 18 to 22 base pair short primers. In addition to nucleic acids, the Biochip Arrayer can spot antibodies, proteins, peptides and bacterial cells as well. We produce arrays of the highest quality but at a low density (up to about 2,000 spots per slide).
We assist all clients in designing the appropriate microarray for your needs. We take the guesswork out of array alignment and maintain a high level of spatial precision throughout the printing run. Specification with regard to array design and spot placement is tightly monitored. This attention to detail results in saved scanner time and fewer auto format failures.
We provide extensive on site training and support for all HybStation, Maui, and Agilentâ€™s Hybridization Oven users.
Scanning and Data Analysis
Support is provided for spotted microarray scanning and for data analysis with a variety of software packages, including GenSifter.
Analysis of RNA, DNA, Proteins and Cells
Quantity and quality of your RNA, DNA, proteins and cells are determined with the help of an Agilent 2100 Bioanalyzer.
We have helped clients with all aspects of their microarray experiments, including devising specific methods for the extraction of high-quality RNA from difficult samples, such as for example the microcrostacean Daphnia (D. Pulex) and Pap test material.
Services are offerred outside of University of South Carolina at Columbia