Search the CoreMarketplace
Searching
All Facilities >> University of South Carolina at Columbia >> South Carolina College of Pharmacy Microarray Core Facility
Facility Details
About This Facility
Services and Equipment
Publications
Associations
Metadata
University of South Carolina at Columbia
Dept. of Pharmaceutical and Biomedical Sciences, University of South Carolina
715 Sumter Street, CLS 617
Columbia, SC 29208
United States of Americahttp://www.sccp.sc.edu/research/mcf/index.aspxDr. Kim E Creek
Last Updated: 11/29/2010
Services Provided: The USC Microarray Core Facility (formerly the SCCC DNA Microarray Facility) can produce Custom Arrays, support Array Design, conduct RNA quality control, amplification and labeling; conduct array hybridization, scanning and data analysis, and assist customers in all aspects of the design and performance of microarray experiments, using Agilent (pre-made and custom) and spotted arrays. In addition, the facility provides support for quantitation and quality assessment of DNA, RNA and protein on an Agilent 2100 Bioanalyzer.
Custom Arrays
The facility is equipped to provide custom microarrays for academic and industrial clients. So far we have successfully spotted cDNAs, 50 to 70 base pair oligonucleotides and 18 to 22 base pair short primers. In addition to nucleic acids, the Biochip Arrayer can spot antibodies, proteins, peptides and bacterial cells as well. We produce arrays of the highest quality but at a low density (up to about 2,000 spots per slide).
Array Design
We assist all clients in designing the appropriate microarray for your needs. We take the guesswork out of array alignment and maintain a high level of spatial precision throughout the printing run. Specification with regard to array design and spot placement is tightly monitored. This attention to detail results in saved scanner time and fewer auto format failures.
Hybridization
We provide extensive on site training and support for all HybStation, Maui, and Agilent’s Hybridization Oven users.
Scanning and Data Analysis
Support is provided for spotted microarray scanning and for data analysis with a variety of software packages, including GenSifter.
Analysis of RNA, DNA, Proteins and Cells
Quantity and quality of your RNA, DNA, proteins and cells are determined with the help of an Agilent 2100 Bioanalyzer.
We have helped clients with all aspects of their microarray experiments, including devising specific methods for the extraction of high-quality RNA from difficult samples, such as for example the microcrostacean Daphnia (D. Pulex) and Pap test material.
Services are offerred outside of University of South Carolina at Columbia
The Facility includes: (1) a Packard Bioscience Biochip Arrayer. Based on Ink jet technology the arrayer has 4 piezoelectric capillary tips that achieve extremely accurate non-contact dispensing. When in operation the glass capillaries of PiezoTip dispensers cruise over a glass slide or other substrate at a height of 500 microns, dispensing droplets of 350 picoliters to a target with a X/Y positional accuracy of 10 microns. The Biochip Arrayer is ideal for the production of extremely high quality arrays of up to about 2,000 spots/slide, custom-made to satisfy the requirements of individual investigators. The arrayer also has enormous flexibility in terms of substrate and spotted product, being able to spot on a variety of materials, and not only nucleic acids but also proteins, peptides, and even bacterial cells; (2) an automatic hybridization system in the form of a 12 position GeneTac HybStation. Up to six protocols can be run simultaneously, which allows for hybridization with uniform, reproducible results and minimal handling of solutions and slides; (3) a hybridization oven and rotator, which are optimized for the hybridization of high-density arrays from Agilent Technologies and a Maui Hybridization System; (4) a Perkin Elmer confocal laser scanner Scan Array Express HT is available for microarray scanning using up to 5 lasers which allow for the detection and quantification of signal from virtually all commercially available fluorochromes including the commonly used Cy3 and Cy5; (5) an Agilent Technologies 2100 Bioanalyzer that allows for the rapid assessment of RNA quality (software available with this instrument assigns an RNA Integrity Number (RIN) to the samples, based on various parameters of RNA integrity; and (6) a powerful 8-tip automated liquid handling system – the Perkin Elmer Multiprobe II - to ensure accuracy and reliability in the handling and arraying of oligonuclotides and cDNAs prior to spotting.
No publications have been associated with this facility
Keywords:
This facility has not been assigned an RRID
Resource Type:
This facility has not been assigned an RRID
Citation:
This facility has not been assigned an RRID