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In Vitro Analytical Core

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Primary Contact:

Ling Cao

2076022213

Last Updated: 05/03/2025

Additional Citation Identifiers

5P20GM152330-02

Facility Details

The in Vitro Analytical Core (IVAC) facilitates the mission of the UNE Center for Cell Signaling Research (CCSR) by identifying molecular mechanisms and cellular processes in aging, stress, bioenergetics, and cellular metabolism by providing the critical expertise and instrumentation to study cell-cell interactions and to delineate intracellular pathways in targeted cell types.
The IVAC provides training, instrumentation, and technical services for the development, analysis, and storage of in vitro cell models (such as stably transfected cell lines using genetically encoded biosensors) across a wide range of biological and biomedical applications, as well as various types of sample preparation for downstream analyses such as mass spectrometry and RNA-seq.
Services:
INSTRUMENTATION
• QuantStudio 3 real-time PCR system
• FLIPR Tetra Fluorescent Imaging Plate Reader
• CellInsight CX7 LZR High Content Analysis Platform
• Agilent Seahorse XF HS Mini
• Mammalian Cell Culture Facilities
• General Protein Analysis Equipment
HANDS-ON SERVICES
• Consultations
• Troubleshooting
• Protocol Development
• Cell Line Maintenance and Banking
TRAINING
• Instrumentation Demos
• Individual Hands-On Training
• Workshops
Contact us at ivac@une.edu for more details.

Funding Info

NIH - 01/04/2025 - In Vitro Analytical Core

is_active:

Yes

budget_end:

12/31/2025

date_added:

01/04/2025

agency_code:

NIH

fiscal_year:

2025

project_num:

5P20GM152330-02

award_amount:

$295,312.00

budget_start:

01/01/2025

org_name:

UNIVERSITY OF NEW ENGLAND

org_state:

ME

org_country:

UNITED STATES

org_zipcode:

040059526

primary_duns:

071735252

abstract_text:

Project Summary The Center for Cell Signaling Research (CCSR) focuses on delineating molecular mechanisms and cellular processes in aging, stress, bioenergetics, and cellular metabolism, which requires the expertise and instrumentation to study cell-cell interactions and to delineate intracellular pathways within a given cell type. To support the goals of the CCRS Research Project Leaders (RPLs), we propose to establish a new In Vitro Analytical Core (IVAC) facility. The IVAC will provide training, instrumentation, and services for the development, analysis, and storage of in vitro cell models for a wide range of biological and medical investigations, as well as various types of sample preparations for downstream analysis. Specifically, the IVAC will focus on the development of a stable cell lines, the performance of transfections that enables the use of genetically encoded biosensors for functional imaging of biochemical processes, the utilization of a wide range of protein analysis approaches (gel/blots/well-based/cell-based), and the preparation of samples for downstream “omics” applications that can be completed by external IDeA cores. The IVAC will be equipped with instrumentation that is currently unavailable in Maine (e.g. the Thermo Scientific CellInsight CX5, a high content multiplexable quantitative imager for cellular/subcellular functional examinations, a QuantStudio5 384-well Real-Time multiplexable PCR machine, and a Thermo iBright 1500FL 5-channel gel/blot imager for multiplex fluorescence analyses), as well as existing shared resources that are currently not part of a research core. All equipment will be consolidated into a centralized, newly renovated laboratory. Dr. Ling Cao, a successful alumnus of a previous COBRE program, will serve as the Core Director and oversees the operation of the IVAC along with a full-time Core Manager and a Staff Technician. To ensure high-quality services and scientific rigor, the IVAC will 1) establish series of standard operation procedures, 2) design and implement new training programs for faculty, staff, and trainees, 3) be engaged in method development/improvement, and 4) grow its collaborations with other core facilities within and outside of UNE. While keeping RPLs as the IVAC’s priority, establishment of the IVAC will also improve UNE’s research capacity and create a regional resource thus benefit investigators within and outside UNE. The IVAC will also work towards long-term sustainability by implementing an efficient online management system, conducting regular evaluations, continuing professional development, and increasing its regional and national visibility and reputation. In addition, UNE has committed vital support to the future success of the IVAC, including renovation costs, matching funds for equipment purchases and service contracts, and additional protected time for the Core Director. The following sequential steps are outlined to enable us to establish the IVAC and accomplish its mission of enhancing research infrastructure and providing quality services to support the research programs of the RPLs and other UNE investigators. Aim 1: Establish a functional IVAC facility; Aim 2: Support RPLs to achieve their project goals through comprehensive services.

project_title:

In Vitro Analytical Core

contact_pi_name:

CAO, LING

total_cost:

$295,312.00

Facility Policies

Services are offerred outside of University of New England

Consulting is offerred outside of University of New England