The BCCHR Imaging Core is a multi-user facility located on the 4th floor in the Translational Research Building (TRB) at A4-110. It houses five powerful microscopes and four image data analysis computers to provide imaging a wide range of samples, including cell structures in tissue sections, and subcellular structures and 3D morphologies of single cells. This allows for high-throughput screening for quick profiling of cells at a large scale, multiplexing imaging over 100 targets, time-lapse imaging to observe cell behaviour in real time, and also super-resolution techniques to resolve structures at the level of single proteins. A full-time technician provides training, consultation, and guidance to researchers, from sample preparation to data analysis.
This facility operates on a fee-for-service basis, which allows for partial cost recovery for operating expenses and service contracts for the instruments. A portion of the facility operating and instrumentation costs are provided through financial support from the BC Children‘s Hospital Research Institute and Foundation, University of British Columbia and Canada Foundation for Innovation (CFI) awards to contributing investigators.
Facility Policies
Services are offerred outside of University of British Columbia
Consulting is offerred outside of University of British Columbia
Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]
Molecular Devices ImageXpress High Content Confocal Imaging System
ImageXpress Micro Confocal system is a high-content solution that can switch between widefield and confocal imaging of fixed and live cells. It can capture high quality images of whole organisms, thick tissues, 2D and 3D models, and cellular or intracellular events. The spinning disc confocal and sCMOS camera enable imaging of fast and rare events like cardiac cell beating and stem cell differentiation. With MetaXpress software and flexible options like water immersion objectives to choose from, the system enables many confocal imaging applications from 3D assay development to screening. [Product Link]
BX61 Upright Wide Field Microscope is specially designed to work with the Olympus laser based autofocus unit. With precise focus and active tracking, users can speed up routine inspections. Additional settings such as illumination level, lens selection and aperture setting can be set using push buttons on the microscope frame, a keypad or via the PC. A variety of motorized modules including nosepieces and illuminators are available to provide you with full flexibility in configuring your system. [Product Link]
Powered by high-speed imaging and proprietary barcoding technology, PhenoCycler-Fusion 2.0 now introduces multi-slide automation with parallel processing of slides. This advanced capability amplifies your ability to uncover more discoveries faster than ever before.
Keyence BZ-X810
The BZ-X810 is a fully automatic inverted fluorescence phase contrast microscope system that incorporates motorized control and imaging functions in a small and compact all-in-one darkroom design, stage/focus/and image capture functions are all controlled via software on an external pc workstation, automatic motorized functions include objective lens switching and focus along the Z-axis, XY-axis stage control, automatic image focus and exposure, and fluorescence filter cube switching, system includes a 6-position revolving nosepiece equipped with 3 Nikon CFI60 objectives; 1) Plan Apo 10x/0.45, 2) Plan Apo 40x/0.95, and 3) Plan Apo 100x/1.45 Oil, fluorescence filter cubes included are DAPI, CY3/TRITC, EYFP, and AlexaFluor 514/ZsYellow/Eosin, comes with the BZ-X800E fluorescent light illumination unit and light guide and 5 total stage inserts for a variety of specimen vessel types, also comes with a Dell laptop computer with BZ-X800 Viewer/Analyzer software installed, 100-240V, 50/60Hz.
No additional equipment has been listed
Publications
No publications have been associated with this facility
