The CoreMarketplace: Bioimaging facility

The UBC Bioimaging facility (BIF) provides researchers with top-end optical and electron microscope infrastructure for live-cell imaging, multi-photon imaging, advanced sample preparation including cryo-preparation for electron microscopy, routine SEM and TEM, Cryo-TEM, TEM tomography, and Correlative Light and Electron Microscopy (CLEM). Currently, the BIF is equipped with 4 electron microscopes (1 SEM and 3 TEMs), 2 advanced fluorescence microscopes (confocal/multi-photon and spinning disk), cryo-fixation (High pressure freezer, Vitrobot) and EM processing equipment (Microwave, critical point dryer, coaters).
The facility is open to everyone and provides microscopy access, training and service. We have three technical staff who all have more than 10 years of experience providing technical knowledge and can support users from diverse research disciplines from life sciences to material sciences.

Facility Policies

Services are offerred outside of University of British Columbia

Consulting is offerred outside of University of British Columbia

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Services offered:

Microscopy

Facility Equipment

Tecnai G2 200kV TEM
Tecnai Spirit 120kV TEM
Hitachi H7600 TEM
Hitachi S4700 SEM
Olympus FV1000 confocal and multi-photon microscope
PerkinElmer spinning disk confocal microscope
Olympus BX53 light/fluorescence microscope
Olympus SZX10 stereomicroscope
Leica HPM100 high-pressure-freezer
Leica AFS2
Leica ACE200
Leica VT1000 vibratome
FEI Vitrobot Mark IV
PELCO 344l Laboratory Microwave System
Tousimis Autosamdri 815B Critical Point Dryer
Cressington 208HR High Resolution Sputter Coater

(To see more facility information, click the in the upper right)

Publications

Publications associated with this facility (Click To View):

1.) Luppi BT, Primrose WL, Hudson ZM (2024 Apr 22). Polymer Dots with Delayed Fluorescence and Tunable Cellular Uptake for Photodynamic Therapy and Time-Gated Imaging. Angewandte Chemie (International ed. in English), 63(17), e202400712. doi: 10.1002/anie.202400712. PMID: 38439710.

2.) Asghari P, Scriven DR, Shahrasebi S, Valdivia HH, Alsina KM, Valdivia CR, Navarro-Garcia JA, Wehrens XH, Moore ED (2024 Feb 22). Phosphorylation of RyR2 simultaneously expands the dyad and rearranges the tetramers The Journal of General Physiology, 156(4), e202213108. . PMCID: 10883851.

3.) Fleetwood SK, Kleiman M, Foster EJ (2024). Preparation of isolated guard cells, containing cell walls, from Vicia faba. PloS one, 19(3), e0299810. . PMID: 38513160.

4.) Leung J, Strong C, Badior KE, Robertson M, Wu X, Meledeo MA, Kang E, Paul M, Sato Y, Harashima H, Cap AP, Devine DV, Jan E, Cullis PR, Kastrup CJ (2023 Dec). Genetically engineered transfusable platelets using mRNA lipid nanoparticles. Science advances, 9(48), eadi0508. . PMID: 38039367.

5.) Ackroyd AJ, De Paolis A, Xu YT, Momeni A, Hamad WY, MacLachlan MJ (2023 Sep 14). Self-assembly of cellulose nanocrystals confined to square capillaries. Nanoscale, 15(35), 14388-14398. doi: 10.1039/d3nr02650g. PMID: 37609826.

6.) Lu Y, Kamkar M, Guo S, Niu X, Wan Z, Xu J, Su X, Fan Y, Bai L, Rojas OJ (2023 Sep). Super-Macroporous Lightweight Materials Templated from Bicontinuous Intra-Phase Jammed Emulsion Gels Based on Nanochitin. Small (Weinheim an der Bergstrasse, Germany), 19(39), e2300686. doi: 10.1002/smll.202300686. PMID: 37147774.

7.) Andrew LJ, Gillman ER, Walters CM, Lizundia E, MacLachlan MJ (2023 Aug). Multi-Responsive Supercapacitors from Chiral Nematic Cellulose Nanocrystal-Based Activated Carbon Aerogels. Small (Weinheim an der Bergstrasse, Germany), 19(34), e2301947. doi: 10.1002/smll.202301947. PMID: 37093171.

8.) Livingston SJ, Chou EY, Quilichini TD, Page JE, Samuels AL (2023 Jul). Overcoming the challenges of preserving lipid-rich Cannabis sativa L. glandular trichomes for transmission electron microscopy. Journal of microscopy, 291(1), 119-127. doi: 10.1111/jmi.13165. PMID: 36542368.

9.) Cai G, Zong Z, Smith KJ, Upham DC (2023 Jun 27). New molten metal catalysts for CO(2) and CH(4) conversion to 2 : 1 H(2) : CO syngas. Chemical communications (Cambridge, England), 59(52), 8143-8146. doi: 10.1039/d3cc02088f. PMID: 37309840.

10.) Cho K, Andrew LJ, MacLachlan MJ (2023 Jun 12). Uniform Growth of Nanocrystalline ZIF-8 on Cellulose Nanocrystals: Useful Template for Microporous Organic Polymers. Angewandte Chemie (International ed. in English), 62(24), e202300960. doi: 10.1002/anie.202300960. PMID: 36869007.

11.) Golla K, Paul M, Lengyell TC, Simpson EM, Falet H, Kim H (2023 May). A novel association between platelet filamin A and soluble N-ethylmaleimide sensitive factor attachment proteins regulates granule secretion. Research and practice in thrombosis and haemostasis, 7(4), 100019. . PMID: 37538498.

12.) Blake AJ, Hung E, To S, Ng G, Qian J, Gries G (2023 Apr 21). Stable flies sense and behaviorally respond to the polarization of light. Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, (), . doi: 10.1007/s00359-023-01624-y. PMID: 37083716.

13.) Burckhardt JC, Chong DHY, Pett N, Tropini C (2023 Mar 30). Gut commensal Enterocloster species host inoviruses that are secreted in vitro and in vivo. Microbiome, 11(1), 65. . PMID: 36991500.

14.) Das M, Cheng D, Matzat T, Auld VJ (2023 Mar 29). Innexin-Mediated Adhesion between Glia Is Required for Axon Ensheathment in the Peripheral Nervous System. The Journal of neuroscience : the official journal of the Society for Neuroscience, 43(13), 2260-2276. . PMID: 36801823.

15.) Behar H, Mottiar Y, Chandrasekhar R, Grappadelli AC, Pauly M, Samuels AL, Mansfield SD, Brumer H (2023 Feb). Populus endo-glucanase 16 localizes to the cell walls of developing tissues. Plant direct, 7(2), e482. . PMID: 36733272.

16.) Guo S, Tao H, Gao G, Mhatre S, Lu Y, Takagi A, Li J, Mo L, Rojas OJ, Chu G (2023 Jan 9). All-Aqueous Bicontinuous Structured Liquid Crystal Emulsion through Intraphase Trapping of Cellulose Nanoparticles. Biomacromolecules, 24(1), 367-376. . PMID: 36479984.

17.) Livingston SJ, Rensing KH, Page JE, Samuels AL (2022 Sep 26). A polarized supercell produces specialized metabolites in cannabis trichomes. Current biology : CB, 32(18), 4040-4047.e4. doi: 10.1016/j.cub.2022.07.014. PMID: 35917819.

18.) Mayder DM, Christopherson CJ, Primrose WL, Lin AS, Hudson ZM (2022 Aug 31). Polymer dots and glassy organic dots using dibenzodipyridophenazine dyes as water-dispersible TADF probes for cellular imaging. Journal of materials chemistry. B, 10(34), 6496-6506. doi: 10.1039/d2tb01252a. PMID: 35979840.

19.) Duan T, Bian Q, Li H (2022 Jul 11). Protein Hydrogels with Reversibly Patterned Multidimensional Fluorescent Images for Information Storage. Biomacromolecules, 23(7), 3009-3016. doi: 10.1021/acs.biomac.2c00499. PMID: 35749455.

20.) Perkins ML, Schuetz M, Unda F, Chen KT, Bally MB, Kulkarni JA, Yan Y, Pico J, Castellarin SD, Mansfield SD, Samuels AL (2022 Apr 26). Monolignol export by diffusion down a polymerization-induced concentration gradient. The Plant cell, 34(5), 2080-2095. . PMID: 35167693.

21.) Livingston SJ, Bae EJ, Unda F, Hahn MG, Mansfield SD, Page JE, Samuels AL (2021 Dec 27). Cannabis Glandular Trichome Cell Walls Undergo Remodeling to Store Specialized Metabolites. Plant & cell physiology, 62(12), 1944-1962. . PMID: 34392368.

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Associations

BINA

Bioimaging North America

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Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=1190
https://coremarketplace.org/RRID:SCR_021304

Institution

Institution ROR ID: https://ror.org/03rmrcq20

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, ABRF, optical microscope, electron microscope, live cell imaging, multi photon imaging, sample preparation, cryo-preparation, SEM, TEM, Cryo-TEM, TEM tomography

Resource Type:

service resource, core facility, access service resource

Citation:

University of British Columbia Vancouver Bioimaging Core Facility (RRID:SCR_021304)

Bioimaging facility (Microscopy (Electron, Fluorescence, Optical))

Primary Contact:

Facility RRID

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

BINA

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Facility Profile QR Code (links to this profile)

(To see more facility information, click the in the upper right)

Contact Miki Fujita

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Bioimaging facility

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